The Transport of Carbon Dioxide by Erythrocytes and Plasma in Dogfish Blood

Ferguson, John; Horváth, Szatmár; Pappenheimer, J. R.

doi:10.2307/1537568

Cited by 24 publications

(7 citation statements)

References 6 publications

(6 reference statements)

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Section: Discussionmentioning

confidence: 75%

“…Calcium and sodium transfer in tortoise erythrocytes Black & Irving (1938) found that if carp's blood were oxalated haemolysis occurred. Similarly, Ferguson, Horvath & Pappenheimer (1938) found that the addition of fluoride caused dog-fish cells to haemolyse, and later this phenomenon was observed by lamdi & Ferguson (1940) with the blood of the tautog, sea-robin and squeteague. Ferguson et at.…”

Section: Effects Of Cell Sodium Concentration ([Nat]) On the Permeabimentioning

confidence: 75%

“…Similarly, Ferguson, Horvath & Pappenheimer (1938) found that the addition of fluoride caused dog-fish cells to haemolyse, and later this phenomenon was observed by lamdi & Ferguson (1940) with the blood of the tautog, sea-robin and squeteague. Ferguson et at. (1938) suggested that the lysis was due to the removal of ionized magnesium, but fluoride will also remove ionized calcium, and in view of Lyman's (1945) observations, it is probable that the removal of Ca2+ is the effective factor in promoting lysis of the erythrocytes examined by Ferguson et al Lyman found that the erythrocytes of the snapping turtle were haemolysed by Ca-free media; the erythrocytes of the golden-striped, box and marine turtles were unaffected by Ca-lack.…”

Section: Effects Of Cell Sodium Concentration ([Nat]) On the Permeabimentioning

confidence: 75%

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Sodium transfer in tortoise erythrocytes

Maizels¹

1956

The Journal of Physiology

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Section: Discussionmentioning

confidence: 75%

Section: Effects Of Cell Sodium Concentration ([Nat]) On the Permeabimentioning

confidence: 75%

Section: Effects Of Cell Sodium Concentration ([Nat]) On the Permeabimentioning

confidence: 75%

See 1 more Smart Citation

Sodium transfer in tortoise erythrocytes

Maizels¹

1956

The Journal of Physiology

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“…It is evident that the best fit of E m calc to E m meas values as seen in Fig. 4 is where the percent volume of the cells occupied by the nucleus is 28.7% (open circles). The quantitative values at pH 7.2 as seen in Fig.…”

Section: Resultsmentioning

confidence: 90%

Fluorescent imaging of Cl ^- in Amphiuma red blood cells: How the nuclear exclusion of Cl ^- affects the plasma membrane potential

Hoffman

Geibel²

2005

Proc. Natl. Acad. Sci. U.S.A.

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In this work, we test the idea that most, if not all, cellular Cl ؊ of Amphiuma red blood cells is contained in the cytoplasm. If true, this could resolve the difference between the measured plasma membrane potential (E m) and that expected from the Donnan equilibrium distribution of Cl ؊ . We studied the changes in the fluorescence intensity of the Cl ؊ -sensitive dye, MQAE, entrapped in red cells that occurred when intracellular Cl ؊ was exchanged with NO 3 ؊ . We could thus monitor the distribution of Cl ؊ between the nuclear and cytoplasmic compartments. We found that essentially all of the cell's Cl ؊ resides in the cytoplasm. Knowing the volume of the cell occupied by the nucleus, we could accordingly correct the measured values of cell Cl ؊ . This resulted in establishing a concordance between the measured values of E m and those calculated from the corrected values of the Cl ؊ ratio, thus explaining the discrepancy. The exclusion of Cl ؊ from the nucleus may result from its unusually high content of ''excess'' DNA that imposes an imbalance of net negative charge.MQAE ͉ nuclear͞cytoplasmic ratios T he equilibrium distribution of permeable ions such as Cl Ϫ across the plasma membrane of red blood cells should be determined by the membrane potential (E m ) that is set by the relative permeability to Na ϩ and K ϩ and their concentration gradients. This paper addresses the E m of Amphiuma red blood cells and the reason why the measured E m appears to differ significantly from that expected from the equilibrium distribution of Cl Ϫ . The idea that the E m of red blood cells in general and of human red cells in particular could be described by a Donnan potential is based on analyses (1-4) of the passive equilibrium distribution of permeable ions between the cytoplasm (Cl i Ϫ ) and the external medium (Cl o ). The E m of human red cells, measured indirectly with the use of fluorescent dyes (5), was found to vary quantitatively with the Donnan ratio of Cl i ͞Cl o (r Cl ). This was so when r Cl was varied either by changes in external pH (pH o ) (5-7) or by altering the concentration of Cl o by substitution with an impermeable anion (5). Validation that the fluorescence technique provided an accurate assessment of E m in human red cells stemmed from the concordance of E m determined directly in Amphiuma red blood cells by impalement with microelectrodes (8, 9, †) with those determined by the use of fluorescent dyes (5). These results established the basis for expecting measurements of E m in Amphiuma red blood cells to conform to a Donnan potential. However, analyses showed that, although the E m of Amphiuma responded to changes in Cl i produced by changes in pH o , the calculated values of E m based on measured values of Cl i and Cl o were significantly higher than the observed E m values. E m was calculated by use of the Nernst equation, E m ϭ 58 log Cl i ͞Cl o , where 58 is the value of RT͞F at 20°C., with R being the gas constant, T the temperature, and F, the Faraday constant. It turns out that the problem li...

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“…Carbamino C 0 2 may or may not constitute a significant component, depending on the species (Ferguson et al 1938;Ferguson and Black 194 1). Heming (1984) has calculated that carbamino C02 accounts for approximately 5% of Cco2 in rainbow trout blood.…”

Section: Sco2mentioning

confidence: 99%

Carbon dioxide excretion in fishes

Perry¹

1986

Can. J. Zool.

126

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The pattern and control of carbon dioxide excretion in fish is reviewed with particular emphasis on the site(s) of bicarbonate dehydration, the involvement of diffusive and convective processes, and the relationship with ionic and acid–base regulation. The principal route for carbon dioxide excretion in fish involves the catalysed dehydration of plasma bicarbonate within erythrocytes to form physically dissolved CO2 and the subsequent diffusion of physically dissolved CO2 across the gill epithelium. It is likely that bicarbonate entry into the erythrocyte in exchange for intracellular chloride, rather than branchial CO2 diffusion or blood/water convection, is the rate-limiting process in carbon dioxide excretion, although a change in any one of these factors will affect overall CO2 elimination. Additionally, a relatively minor amount of CO2 is hydrated within gill epithelial cells to form H+ and HCO3− ions that are exchanged for Cl− ions and Na+ ions, respectively. Evidence is presented indicating that branchial and erythrocytic HCO3−/Cl− exchanges are under adrenergic control and that modulations of these processes by elevated levels of circulating catecholamines may be important in regulating acid–base disturbances.

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The Transport of Carbon Dioxide by Erythrocytes and Plasma in Dogfish Blood

Cited by 24 publications

References 6 publications

Sodium transfer in tortoise erythrocytes

Sodium transfer in tortoise erythrocytes

Fluorescent imaging of Cl ^- in Amphiuma red blood cells: How the nuclear exclusion of Cl ^- affects the plasma membrane potential

Carbon dioxide excretion in fishes

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The Transport of Carbon Dioxide by Erythrocytes and Plasma in Dogfish Blood

Cited by 24 publications

References 6 publications

Sodium transfer in tortoise erythrocytes

Sodium transfer in tortoise erythrocytes

Fluorescent imaging of Cl - in Amphiuma red blood cells: How the nuclear exclusion of Cl - affects the plasma membrane potential

Carbon dioxide excretion in fishes

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Fluorescent imaging of Cl ^- in Amphiuma red blood cells: How the nuclear exclusion of Cl ^- affects the plasma membrane potential