The translational roadmap of the gut models, focusing on gut-on-chip

Abstract

It is difficult to model in vitro the intestine when seeking to include crosstalk with the gut microbiota, immune and neuroendocrine systems. Here we present a roadmap of the current models to facilitate the choice in preclinical and translational research with a focus on gut-on-chip. These micro physiological systems (MPS) are microfluidic devices that recapitulate in vitro the physiology of the intestine. We reviewed the gut-on-chips that had been developed in academia and industries as single chip and that have three main purpose: replicate the intestinal physiology, the intestinal pathological features, and for pharmacological tests.

Gut Dysbiosis in Irritable Bowel Syndrome: A Narrative Review on Correlation with Disease Subtypes and Novel Therapeutic Implications

“…Several emerging microbiota-directed therapeutic approaches warrant further exploration for IBS. These include engineered probiotic bacterial strains, bacteriophage therapy, stem cell microfluidic intestine-on-a-chip models, and CRISPR-Cas9 gene editing of the gut microbiota [196,197]. By targeting specific dysbiotic microbes or their effects, such innovative strategies may translate advances in IBS pathophysiology into microbiota-mediated clinical management.…”

Intestinal Membrane Function in Inflammatory Bowel Disease

“…Against this background, it is essential to develop an in vitro IBD model that can recapitulate the contributing factors to the maximum extent possible and reconstruct the structure and microenvironment of the intestine [ 124 , 125 ]. However, it has been challenging to develop a novel in vitro gut model to mimic interactions with the microbiota [ 126 ]. One example is a co-culture model of colorectal-cancer-derived epithelial cells with a model probiotic/synbiotic regimen against colorectal cancer [ 127 ].…”