The Transillumination Possibility of Imidazole–Osmium 
Postfixed Tissue and Its Consequences for the Handling of Tissue 
Samples

Voigt, Tilman; Dauber, Wolfgang

doi:10.1017/s1431927605050117

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…It has been demonstrated that the fixation procedure has a significant influence on the morphology of fish maculae, and that vesicular bodies in fish maculae represent artefacts of the fixation procedure . Osmium post‐fixation also results in the blackening of samples, which renders them useless for optical microscopy examination . The use of imidazole‐osmium post fixation was proposed in order to avoid this problem .…”

Section: Nanomaterials and Nanomaterial–organism Interaction Charactermentioning

confidence: 99%

“…Osmium post‐fixation also results in the blackening of samples, which renders them useless for optical microscopy examination . The use of imidazole‐osmium post fixation was proposed in order to avoid this problem . Other fixation agents, such as ruthenium red, could be used instead of osmium tetroxide.…”

Section: Nanomaterials and Nanomaterial–organism Interaction Charactermentioning

confidence: 99%

See 1 more Smart Citation

Toxicity of Metal Oxide Nanoparticles: Mechanisms, Characterization, and Avoiding Experimental Artefacts

Djurišić

Leung

et al. 2014

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341

176

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Metal oxide nanomaterials are widely used in practical applications and represent a class of nanomaterials with the highest global annual production. Many of those, such as TiO2 and ZnO, are generally considered non-toxic due to the lack of toxicity of the bulk material. However, these materials typically exhibit toxicity to bacteria and fungi, and there have been emerging concerns about their ecotoxicity effects. The understanding of the toxicity mechanisms is incomplete, with different studies often reporting contradictory results. The relationship between the material properties and toxicity appears to be complex and diifficult to understand, which is partly due to incomplete characterization of the nanomaterial, and possibly due to experimental artefacts in the characterization of the nanomaterial and/or its interactions with living organisms. This review discusses the comprehensive characterization of metal oxide nanomaterials and the mechanisms of their toxicity.

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Section: Nanomaterials and Nanomaterial–organism Interaction Charactermentioning

confidence: 99%

Section: Nanomaterials and Nanomaterial–organism Interaction Charactermentioning

confidence: 99%

Toxicity of Metal Oxide Nanoparticles: Mechanisms, Characterization, and Avoiding Experimental Artefacts

Djurišić

Leung

et al. 2014

Small

341

176

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“…After rinsing off the fixation solution with 0.1 M cacodylate-buffer the samples were postfixed in 1% osmium-tetroxide in 0.1 M cacodylate-buffer for 1 h. Following dehydration with ethanol, the tissue was embedded in EPON (for technical details see Voigt and Dauber, 2005). After perfusion fixation and preparation of the intestine, the jejunum was minced to small block of 1 mm size.…”

Section: Methodsmentioning

confidence: 99%

“…Thereafter the specimens were fixed for another 2 h in the same fixation solution. After rinsing off the fixation solution with 0.1 M cacodylate-buffer the samples were postfixed in 1% osmium-tetroxide in 0.1 M cacodylate-buffer for 1 h. Following dehydration with ethanol, the tissue was embedded in EPON (for technical details see Voigt and Dauber, 2005). During the embedding the jejunum was oriented to get the cross-sectional aspect.…”

Section: Methodsmentioning

confidence: 99%

Implementation of a virtual correlative light and transmission electron microscope

Voigt

Zuber

Gawatz³

et al. 2013

Microsc. Res. Tech.

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In the long run, the widespread use of slide scanners by pathologists requires an adaptation of teaching methods in histology and cytology in order to target these new possibilities of image processing and presentation via the internet. Accordingly, we were looking for a tool with the possibility to teach microscopic anatomy, histology, and cytology of tissue samples which would be able to combine image data from light and electron microscopes independently of microscope suppliers. With the example of a section through the villus of jejunum, we describe here how to process image data from light and electron microscopes in order to get one image-stack which allows a correlation of structures from the microscopic anatomic to the cytological level. With commercially available image-presentation software that we adapted to our needs, we present here a platform which allows for the presentation of this new but also of older material independently of microscope suppliers.

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“…Osmium postfixation, dehydration using ethanol, and drying using the HMDS techniques have been chosen in our previous study to obtain a better quality of cervical cells in terms of qualitative and quantitative than the osmium postfixation, long-chain ethanol series, and CPD techniques [9]. However, osmium postfixation is known to produce blackening of the specimens [13], especially lipid contents in the cell [14], resulting in reduced differences in contrast and 3-D character of the differentiated cells [8]. Therefore, by avoiding the use of toxic osmium tetroxide for postfixation, we have developed a protocol for the preparation of cervical cells for FE-SEM/EDX analysis, which is safer and cost-effective in order to be used as the first step in our study.…”

mentioning

confidence: 99%

A protocol for Enhanced imaging and Quantification of Cervical Cell Under Scanning electron Microscope

Jusman

Jamal

Valzon

et al. 2019

Int. J. Art. Intell. Research

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The application of Field Emission Scanning Electron Microscopy and Energy Dispersive X-Ray (FE-SEM/EDX) for the characterization of biological samples can produce promising results for classification purpose. The limitations of the established sample preparation technique of cervical cells for FE-SEM/EDX study that differentiate between normal and abnormal cells prompted the development of a proposed protocol for the preparation of cervical cells. The proposed protocol was conducted by a McDowell-Trump fixative prepared in 0.1M phosphate buffer without osmium tetroxide at 4°C for 2 h in the fixation process. Morphologically, the cervical cells scanned under the FE-SEM/EDX did not present blackening effects, and the structure of the cells was not broken based on the FE-SEM images. Quantitatively, the possible elemental distributions in the cells, such as carbon, nitrogen, oxygen, and sodium, are detected in samples prepared by the proposed protocol. The analysed elements were validated using the Attenuated Total Reflection and Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, by avoiding osmium tetroxide fixation, the time required for sample preparation decreased significantly. This sample preparation protocol can be used for normal and abnormal cervical cells in achieving better results in terms of morphological, detected elemental distribution, and rapid in time.

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The Transillumination Possibility of Imidazole–Osmium Postfixed Tissue and Its Consequences for the Handling of Tissue Samples

Cited by 5 publications

References 6 publications

Toxicity of Metal Oxide Nanoparticles: Mechanisms, Characterization, and Avoiding Experimental Artefacts

Toxicity of Metal Oxide Nanoparticles: Mechanisms, Characterization, and Avoiding Experimental Artefacts

Implementation of a virtual correlative light and transmission electron microscope

A protocol for Enhanced imaging and Quantification of Cervical Cell Under Scanning electron Microscope

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