The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production

Ju, Huiming; Zhang, Jiaqing; Bai, Lei; Mu, Yulian; Du, Yutao; Yang, Wenxian; Li, Yong; Sheng, Anzhi; Li, Kui

doi:10.1038/srep10152

Cited by 14 publications

(10 citation statements)

References 58 publications

(68 reference statements)

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“…Pig models are often favored over rodent models due to their clinical relevance and high similarity to human physiology and anatomy 1 2 3 . Traditionally, transgenic pigs have been produced by integrating a gene sequence or a transgene into the genome in a random manner, in which the transgene can be inserted anywhere in the genome 4 . Random integration of a transgene often leads to unstable phenotypes, gene silencing and unpredictable gene expression, and in some cases, this process is mutagenic.…”

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confidence: 99%

Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs

Ruan

et al. 2015

Sci Rep

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118

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Transgenic pigs play an important role in producing higher quality food in agriculture and improving human health when used as animal models for various human diseases in biomedicine. Production of transgenic pigs, however, is a lengthy and inefficient process that hinders research using pig models. Recent applications of the CRISPR/Cas9 system for generating site-specific gene knockout/knockin models, including a knockout pig model, have significantly accelerated the animal model field. However, a knockin pig model containing a site-specific transgene insertion that can be passed on to its offspring remains lacking. Here, we describe for the first time the generation of a site-specific knockin pig model using a combination of CRISPR/Cas9 and somatic cell nuclear transfer. We also report a new genomic “safe harbor” locus, named pH11, which enables stable and robust transgene expression. Our results indicate that our CRISPR/Cas9 knockin system allows highly efficient gene insertion at the pH11 locus of up to 54% using drug selection and 6% without drug selection. We successfully inserted a gene fragment larger than 9 kb at the pH11 locus using the CRISPR/Cas9 system. Our data also confirm that the gene inserted into the pH11 locus is highly expressed in cells, embryos and animals.

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confidence: 99%

Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs

Ruan

et al. 2015

Sci Rep

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118

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“…The special interest assigned to GH is probably due to husbandry in which several land-living and aquatic animals have been genetically modified to excrete more GH and thus could grow faster to slaughtering size, e.g. coho salmon (Oncorhynchus kisutch) (Raven et al, 2008) and pig (Ju et al, 2015). Several studies have been conducted with both transgenic and hormone-implanted trout and coho salmon.…”

Section: Discussionmentioning

confidence: 99%

Hormones as adaptive control systems in juvenile fish

et al. 2020

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Growth is an important theme in biology. Physiologists often relate growth rates to hormonal control of essential processes. Ecologists often study growth as a function of gradients or combinations of environmental factors. Fewer studies have investigated the combined effects of environmental and hormonal control on growth. Here, we present an evolutionary optimization model of fish growth that combines internal regulation of growth by hormone levels with the external influence of food availability and predation risk. The model finds a dynamic hormone profile that optimizes fish growth and survival up to 30 cm, and we use the probability of reaching this milestone as a proxy for fitness. The complex web of interrelated hormones and other signalling molecules is simplified to three functions represented by growth hormone, thyroid hormone and orexin. By studying a range from poor to rich environments, we find that the level of food availability in the environment results in different evolutionarily optimal strategies of hormone levels. With more food available, higher levels of hormones are optimal, resulting in higher food intake, standard metabolism and growth. By using this fitnessbased approach we also find a consequence of evolutionary optimization of survival on optimal hormone use. Where foraging is risky, the thyroid hormone can be used strategically to increase metabolic potential and the chance of escaping from predators. By comparing model results to empirical observations, many mechanisms can be recognized, for instance a change in pace-oflife due to resource availability, and reduced emphasis on reserves in more stable environments. This article has an associated First Person interview with the first author of the paper.

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“…Although the amount of food intake and the ratio of food intake to metabolic body mass as indicated by RFBW were reduced in both FSC-S/normal and FSC-S/HCL mice, their FEI values markedly increased as compared with the corresponding un-supplemented animals. This indicated that some ingested food/energy is not converted into body mass in both normal and HCL mice supplemented with FSC-S. FEI (weight gain per unit of food ingested) is an index reflecting the amount of feed to produce one kilogram of meat in animal husbandry industry [ 32 – 36 ]. In this study, FEI reflects the FSC-S-induced body weight loss in normal and HCL mice.…”

Section: Discussionmentioning

confidence: 99%

A comparative study between Wuweizi seed and its post-ethanol extraction residue in normal and hypercholesterolemic mice

Chu

Pan

et al. 2015

Lipids Health Dis

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BackgroundAt the present, a shift from drug therapy, especially herbal therapy, to dietary supplementation is a trend in the management of dyslipidemia and related diseases. Therefore, the optimal utilization of herbal resource is important for a sustainable development of herbal medicine. Here, we compared the effects of dietary supplementation with Chinese medicine Schisandrae Chinensis Fructus seed (FSC-S) and the post-ethanol extraction residue of FSC-S (FSC-SpEt) on normal diet-fed (normal) and experimental hypercholesterolemic (HCL) mice.MethodsMale ICR mice (n = 10 in each group), weighing 17–21 g, were fed with normal diet (ND) or high cholesterol/bile salt (1/0.3 %, w/w) diet (HCBD) with or without supplemented with FSC-S, FSC-SpEt), or lipid-lowering agent fenofibrate (FF). Ten days later, serum/hepatic lipid and glucose (GLU) levels, body weight, organ/epididymal fat masses, and food/water intake were measured. Lipid level measurements included those of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), HDL/LDL ratio, LDL/HDL ratio, and non-HDL (N-HDL).ResultsSupplementation with FSC-S and FSC-SpEt increased serum TC (by 64 and 25 %, respectively) and LDL (by 60 and 27 %, respectively) in normal mice. FSC-S supplementation elevated serum TC, TG, HDL, LDL, and LDL/HDL ratio (up to 64, 118, 77, 197, and 51 %, respectively) in HCL mice. FSC-SpEt supplementation reduced serum TG (by 15 %) and LDL/HDL ratio (by 18 %), as well as increased serum HDL (by 22 %) and HDL/LDL ratio (by 21 %) in HCBD-fed mice. FSC-S decreased hepatic TC (by 19 %) contents and increased hepatic TG contents by 14 % in normal mice. FSC-S reduced hepatic GLU level in both normal and HCL mice by 24 and 22 %, respectively. Hepatic TC and TG contents were lowered in FSC-SpEt-supplemented normal mice by 16 and 20 %, respectively. The body/fatty masse and food intake were lowered, but the feed efficiency index (FEI), weight gain per unit of food ingested, was increased in FSC-S-supplemented normal and HCL mice. FF supplements reduced serum/hepatic lipids, hepatic GLU contents, and epididymal fat mass, but it induced hepatomegaly and high serum alanine aminotransferase (ALT) activity in normal and/or HCL mice.ConclusionThe ensemble of results indicated that while FSC-SpEt supplementation is beneficial for the treatment of hyperlipidemia/fatty liver, FSC-S is potentially useful for the management of overweight/obesity.

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The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production

Cited by 14 publications

References 58 publications

Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs

Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs

Hormones as adaptive control systems in juvenile fish

A comparative study between Wuweizi seed and its post-ethanol extraction residue in normal and hypercholesterolemic mice

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