The Transcriptome of a Human Polar Body Accurately Reflects Its Sibling Oocyte

Reich, Adrian; Klatsky, Peter C.; Carson, Sandra Ann; Wessel, Gary M.

doi:10.1074/jbc.m111.289868

Cited by 51 publications

(52 citation statements)

References 23 publications

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“…They analyzed over 12,700 unique mRNAs and miRNAs from oocyte samples and compared them with 5,431 mRNAs recovered from the sibling PBs. Their results demonstrated that detection and quantification of mRNA in human PBs is possible and that the human PB mRNA transcriptome reflects that of its sibling MII oocyte [9]. This work and our data demonstrated that PB 1 mRNA is being considered as a proxy for the oocyte [29].…”

Section: Discussionsupporting

confidence: 65%

“…The polar body (PB) is a cell created by asymmetric division of the oocyte at the time of meiosis and produces a readily accessible test source of genomic material that can be sampled as a proxy for the "sibling" oocyte for diagnostic purposes. Reich et al demonstrated that the transcriptome of a human PB accurately reflects its sibling oocyte [8,9]. The ability to quantify mRNA in individual cells-as small as a single PBopens up the possibility that we can detect and compare individual differences in gene expression in the PB without harming the oocyte.…”

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confidence: 99%

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Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Jiao

Woodruff

2014

J Assist Reprod Genet

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Purpose To confirm that aneuploidy candidate genes are detectable in the first polar body (PB 1 ) of MII oocytes and to investigate the age-dependent molecular changes in PB 1 . Methods Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and the first PB was removed. mRNA from each PB and its sibling oocyte was reverse transcribed. Real-time PCR was performed to quantify the expression of six genes (BUB1, CDC20, Filia, MCAK, SGOL1, SMC1A) in single PB. Results We first demonstrated that detection and quantification of transcripts associated with aneuploidy in single mouse oocyte and sibling PB 1 is possible and the relative abundance of mRNA transcripts in a single PB faithfully reflects the relative abundance of that transcript in its sibling oocyte. We further found that transcript levels were significantly lower in aged PBs compared with young PBs (P<0.05). Conclusions Our results suggest that the detection and analysis of polar body mRNA may provide insight in age-related aneuploidy in oocyte. This analysis is a novel concept to investigate the genesis of chromosome abnormality and could potentially assist in the characterization of mechanisms underlying key molecular origin of female meiotic aneuploidy, which would be of great scientific and clinical value.

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Section: Discussionsupporting

confidence: 65%

mentioning

confidence: 99%

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Jiao

Woodruff

2014

J Assist Reprod Genet

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“…Unfortunately, this type of assessment cannot determine the expression levels of essential genes, nor can it consistently predict the developmental potential of the egg (Rienzi et al, 2011;Wang and Sun, 2007). Recent work from Reich et al (2011) suggests that this hurdle can be overcome by biopsy of the first polar body followed by single-cell transcriptomics to determine the expression of genes known to be crucial for oogenesis. As gene expression in the polar body closely reflects expression in the oocyte from which it derived, the polar body can act as a proxy when choosing oocytes for ART.…”

Section: Understanding Reproductive Senescence In Womenmentioning

confidence: 99%

The developmental origins of the mammalian ovarian reserve

2015

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The adult mammalian ovary is devoid of definitive germline stem cells. As such, female reproductive senescence largely results from the depletion of a finite ovarian follicle pool that is produced during embryonic development. Remarkably, the crucial nature and regulation of follicle assembly and survival during embryogenesis is just coming into focus. This developmental pathway involves the coordination of meiotic progression and the breakdown of germ cell cysts into individual oocytes housed within primordial follicles. Recent evidence also indicates that genetic and environmental factors can specifically perturb primordial follicle assembly. Here, we review the cellular and molecular mechanisms by which the mammalian ovarian reserve is established, highlighting the presence of a crucial checkpoint that allows survival of only the highest-quality oocytes.

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“…Although embryo morphology has been correlated with implantation potential [6], selecting embryos with the appropriate morphological appearance alone is not sufficient to guarantee embryo viability and improve the chances of pregnancy. Several methods, such as removing the polar body [34] and one blastomere from the embryo on the third day of culture [28], were proposed to evaluate oocyte and embryo development, although it is not known what impact these techniques have on embryonic development. Therefore, new non-invasive approaches in oocyte and embryo metabolomics appear to be an alternative for gamete selection and have been proposed for assessing the potential of oocytes and embryos using embryo culture media, follicular fluid and cells [6].…”

Section: Introductionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

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Purpose This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. Method Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. Results S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the nonpregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. Conclusion We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.

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The Transcriptome of a Human Polar Body Accurately Reflects Its Sibling Oocyte

Cited by 51 publications

References 23 publications

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

The developmental origins of the mammalian ovarian reserve

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

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