The transcriptome enables the identification of candidate genes behind medicinal value of Drumstick tree (Moringa oleifera)

Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

“…As per the study conducted by Al‐Juhaimi, Ghafoor, Mohamed Ahmed, et al (), amino acids profile in leaves and seeds (g/100 g, respectively) of MO is glumatic acid (2.660 ± 0.13; 3.724 ± 0.18), aspartic acid (2.185 ± 0.06; 3.059 ± 0.02), leucine (2.070 ± 0.15; 2.898 ± 0.22), arginine (1.820 ± 0.06; 2.548 ± 0.08), alanine (1.605 ± 0.33; 2.247 ± 0.46), phenylalanine (1.595 ± 0.06; 2.233 ± 0.09), lysine (1.540 ± 0.14; 2.156 ± 0.20), glycine (1.450 ± 0.11; 2.030 ± 0.16), valine (1.345 ± 0.12; 1.883 ± 0.17), proline (1.280 ± 0.11; 1.792 ± 0.16), threonine (1.265 ± 0.13; 1.771 ± 0.19), isoleucine (1.155 ± 0.03; 1.617 ± 0.05), serine (1.060 ± 0.04; 1.484 ± 0.06), tyrosine (0.915 ± 0.08; 1.281 ± 0.11), histidine (0.730 ± 0.03; 1.022 ± 0.04), hydroxylysine (0.690 ± 0.04; 0.966 ± 0.06), methionine (0.560 ± 0.08; 0.784 ± 0.12), tryptophan (0.510 ± 0.03; 0.714 ± 0.04), cysteine (0.280 ± 0.04; 0.392 ± 0.06), taurine (0.105 ± 0.02; 0.147 ± 0.03), hydroxyproline (0.100 ± 0.01; 0.140 ± 0.02), and ornithine (0.060 ± 0.01; 0.084 ± 0.02), whereas glutamic acid, aspartic acid, argentine, proline, and glycien are major amino acids in seed kernel (Stadtlander & Becker, ). A very recent study (Pasha et al, ) carried out for quantification of the important molecules in different parts of the tree and an understanding of the protein chain reaction that leads to creation of such molecules. Enzymes in the biosynthesis of vitamins and metabolites like quercetin and kaempferol are highly expressed in leaves, flowers, and seeds.…”

“…The expression of Fe, Zn, Mg transporters, and Ca storage proteins were observed in root and leaves, respectively. In general, leaves retain the highest amount of small molecules of human interest to combat the malnutrition (Pasha et al, ).…”

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

“…Furthermore, the existence of Se in the MOSs seeds plays a vital role by protecting from cancer, cardiovascular disease, cognitive decline and thyroid disease. Moreover, these seeds exhibit a powerful antibacterial activity due to the availability of the detected elements including Ca, K, Mg, P, S, Fe, Mn, Zn, Na and Se [42].…”

“…Conversely, the inhibition zones of the E. coli were ranged from 6 to 20 mm wherein the maximum and minimum inhibition zone diameter was obtained with the extracts concentration of 250 and 50 mg/ml, respectively (Fig 9). The observed discrepancy in the bactericidal action of the MOSs extracts for two different types of test bacteria may be due to the occurrence of their varying cell components [42].…”

A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways

“…In this paper, we describe an integrative approach to identify enzymes involved in the biosynthesis of secondary metabolites, using closely related homologues for sequence searches, integrated with evolutionary analysis and validation. The method was employed for the identification of candidate proteins involved in the biosynthesis of several secondary metabolites, vitamins and mineral transporters in the transcriptome of M. oleifera [1] . The steps involved in this unique pipeline have been described below (Please see Graphical abstract).…”