The Transcriptional Regulator MucR, but Not Its Controlled Acid-Activated Chaperone HdeA, Is Essential for Virulence and Modulates Surface Architecture and Properties in Brucella ovis PA

Tartilán-Choya, Beatriz; Sidhu-Muñoz, Rebeca S; Vizcaı́no, Nieves

doi:10.3389/fvets.2021.814752

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…All these data confirm that mucR is crucial for both functioning of free-living B. canis cells and their virulence to hosts [ 41 ]. Similar findings were reported in 2022 for MucR of another representative of Brucella species, B. ovis [ 42 ], which is a non-zoonotic bacterium causing contagious epididymitis and other lesions in rams, which leads to significant economic losses in sheep-breeding areas. It is naturally rough due to the lack of O chains in LPS.…”

Section: Pathogenic Bacteriasupporting

confidence: 86%

The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions

Janczarek

2022

IJMS

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Ros/MucR is a widespread family of bacterial zinc-finger-containing proteins that integrate multiple functions, such as symbiosis, virulence, transcription regulation, motility, production of surface components, and various other physiological processes in cells. This regulatory protein family is conserved in bacteria and is characterized by its zinc-finger motif, which has been proposed as the ancestral domain from which the eukaryotic C2H2 zinc-finger structure has evolved. The first prokaryotic zinc-finger domain found in the transcription regulator Ros was identified in Agrobacterium tumefaciens. In the past decades, a large body of evidence revealed Ros/MucR as pleiotropic transcriptional regulators that mainly act as repressors through oligomerization and binding to AT-rich target promoters. The N-terminal domain and the zinc-finger-bearing C-terminal region of these regulatory proteins are engaged in oligomerization and DNA binding, respectively. These properties of the Ros/MucR proteins are similar to those of xenogeneic silencers, such as H-NS, MvaT, and Lsr2, which are mainly found in other lineages. In fact, a novel functional model recently proposed for this protein family suggests that they act as H-NS-‘like’ gene silencers. The prokaryotic zinc-finger domain exhibits interesting structural and functional features that are different from that of its eukaryotic counterpart (a βββα topology), as it folds in a significantly larger zinc-binding globular domain (a βββαα topology). Phylogenetic analysis of Ros/MucR homologs suggests an ancestral origin of this type of protein in α-Proteobacteria. Furthermore, multiple duplications and lateral gene transfer events contributing to the diversity and phyletic distribution of these regulatory proteins were found in bacterial genomes.

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Section: Pathogenic Bacteriasupporting

confidence: 86%

The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions

Janczarek

2022

IJMS

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“…Another important factor that substantially influences antigenicity of a protein synthesized as a result of vaccination is the stability of this protein after synthesis, including conformational or fold stability in terms of maintaining its three dimensional and biologically active form [ 126 ]. Normally, such the stability and ability to initiate immune response of viral antigen is achieved by the assistance of the molecular chaperones [ 127 , 128 ]. Consequently, adding to a mRNA vaccine sequence(s) of other proteins that stabilize the main antigen and prevent its folding, can enhance the efficacy of the vaccine.…”

Section: Covid-19 Nucleic Acid-based Vaccinesmentioning

confidence: 99%

Nanotechnology-Based RNA Vaccines: Fundamentals, Advantages and Challenges

Pozharov

Minko

2023

Pharmaceutics

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Over the past decades, many drugs based on the use of nanotechnology and nucleic acids have been developed. However, until recently, most of them remained at the stage of pre-clinical development and testing and did not find their way to the clinic. In our opinion, the main reason for this situation lies in the enormous complexity of the development and industrial production of such formulations leading to their high cost. The development of nanotechnology-based drugs requires the participation of scientists from many and completely different specialties including Pharmaceutical Sciences, Medicine, Engineering, Drug Delivery, Chemistry, Molecular Biology, Physiology and so on. Nevertheless, emergence of coronavirus and new vaccines based on nanotechnology has shown the high efficiency of this approach. Effective development of vaccines based on the use of nucleic acids and nanomedicine requires an understanding of a wide range of principles including mechanisms of immune responses, nucleic acid functions, nanotechnology and vaccinations. In this regard, the purpose of the current review is to recall the basic principles of the work of the immune system, vaccination, nanotechnology and drug delivery in terms of the development and production of vaccines based on both nanotechnology and the use of nucleic acids.

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MucR protein: Three decades of studies have led to the identification of a new H‐NS‐like protein

Baglivo,

Malgieri,

Roop

et al. 2024

Molecular Microbiology

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MucR belongs to a large protein family whose members regulate the expression of virulence and symbiosis genes in α‐proteobacteria species. This protein and its homologs were initially studied as classical transcriptional regulators mostly involved in repression of target genes by binding their promoters. Very recent studies have led to the classification of MucR as a new type of Histone‐like Nucleoid Structuring (H‐NS) protein. Thus this review is an effort to put together a complete and unifying story demonstrating how genetic and biochemical findings on MucR suggested that this protein is not a classical transcriptional regulator, but functions as a novel type of H‐NS‐like protein, which binds AT‐rich regions of genomic DNA and regulates gene expression.

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The Transcriptional Regulator MucR, but Not Its Controlled Acid-Activated Chaperone HdeA, Is Essential for Virulence and Modulates Surface Architecture and Properties in Brucella ovis PA

Cited by 7 publications

References 41 publications

The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions

The Ros/MucR Zinc-Finger Protein Family in Bacteria: Structure and Functions

Nanotechnology-Based RNA Vaccines: Fundamentals, Advantages and Challenges

MucR protein: Three decades of studies have led to the identification of a new H‐NS‐like protein

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