The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

Pelkmans, Jordi F.; Vos, Aurin M.; Scholtmeijer, Karin; Hendrix, Ed; Baars, J.J.P.; Gehrmann, Thies; Reinders, Marcel J. T.; Lugones, Luis G.; Wösten, Han A. B.

doi:10.1007/s00253-016-7574-9

Cited by 43 publications

(35 citation statements)

References 23 publications

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“…S5). This is consistent with previous RNA-Seq based reports (Pelkmans et al, 2017) in Schizophyllum and other species (Morin et al, 2012;Plaza et al, 2014;Zhou et al, 2014;Pelkmans et al, 2016), except for hom1 and gat1, which, in the present data, behaved differently, probably due to the different resolution of developmental stage data. The expression profiles of all eight genes were similar between A. ampla and S. commune.…”

Section: Conserved Patterns Of Transcription Factor Expressionsupporting

confidence: 93%

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

et al. 2019

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Summary Agaricomycetes are fruiting body‐forming fungi that produce some of the most efficient enzyme systems to degrade wood. Despite decades‐long interest in their biology, the evolution and functional diversity of both wood‐decay and fruiting body formation are incompletely known. We performed comparative genomic and transcriptomic analyses of wood‐decay and fruiting body development in Auriculariopsis ampla and Schizophyllum commune (Schizophyllaceae), species with secondarily simplified morphologies, an enigmatic wood‐decay strategy and weak pathogenicity to woody plants. The plant cell wall‐degrading enzyme repertoires of Schizophyllaceae are transitional between those of white rot species and less efficient wood‐degraders such as brown rot or mycorrhizal fungi. Rich repertoires of suberinase and tannase genes were found in both species, with tannases restricted to Agaricomycetes that preferentially colonize bark‐covered wood, suggesting potential complementation of their weaker wood‐decaying abilities and adaptations to wood colonization through the bark. Fruiting body transcriptomes revealed a high rate of divergence in developmental gene expression, but also several genes with conserved expression patterns, including novel transcription factors and small‐secreted proteins, some of the latter which might represent fruiting body effectors. Taken together, our analyses highlighted novel aspects of wood‐decay and fruiting body development in an important family of mushroom‐forming fungi.

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Section: Conserved Patterns Of Transcription Factor Expressionsupporting

confidence: 93%

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

et al. 2019

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“…Although TF families were usually not conserved, we found five TF families that contained developmentally regulated genes from five or six species (Dataset S10). These included C2H2-type zinc fingers [including c2h2 of Schizophyllum (18, 57)], and Zn(2)-C6 fungal-type and homeobox TFs [containing hom1 of Schizophyllum (18,57)]. Two clusters of C2H2 and homeobox TFs showed expression peaks in stipes of Coprinopsis, Lentinus, Armillaria, and Rickenella, confirming previous reports of Hom1 expression in Coprinopsis (58) and Schizophyllum (57,59).…”

Section: Targeted Protein Degradation Shows Striking Expansion In Mush-supporting

confidence: 82%

“…These included C2H2-type zinc fingers [including c2h2 of Schizophyllum (18, 57)], and Zn(2)-C6 fungal-type and homeobox TFs [containing hom1 of Schizophyllum (18,57)]. Two clusters of C2H2 and homeobox TFs showed expression peaks in stipes of Coprinopsis, Lentinus, Armillaria, and Rickenella, confirming previous reports of Hom1 expression in Coprinopsis (58) and Schizophyllum (57,59). Members of the light receptor white collar complex were developmentally regulated in all species except Phanerochaete, mostly showing a significant increase in expression at initiation.…”

Section: Targeted Protein Degradation Shows Striking Expansion In Mush-mentioning

confidence: 99%

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi

Krizsán

Almási

Merényi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

130

364

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The evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple multicellular aggregates of cells, it has evolved only in a handful of lineages, including animals, embryophytes, red and brown algae, and fungi. Despite being a key step toward the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. The development of fungal fruiting bodies from a hyphal thallus represents a transition from simple to complex multicellularity that is inducible under laboratory conditions. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall remodeling, targeted protein degradation, signal transduction, adhesion, and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, many of which convergently expanded in multicellular plants and/or animals too, reflecting convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. This study provides an entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms. complex multicellularity | evolution | fungi | comparative genomics | fruiting body development F ungi represent a diverse lineage of complex multicellular organisms with a unique evolutionary history compared with complex multicellular animals, embryophytes, florideophytes, and laminarean brown algae (1-4). Within the fungal kingdom, complex multicellularity is discussed mostly in the context of fruiting bodies, which are found in at least eight independent lineages (2), of which the Pezizomycotina (Ascomycota) and the Agaricomycetes (Basidiomycota) contain the vast majority of species. The mushroom-forming fungi (Agaricomycetes) comprise >21,000 species and originated 350 million years ago (5), approximately coinciding with the origin of tetrapods. Fruiting bodies of mushroom-forming fungi have immense importance in agriculture, ecology, and medicine; they represent an important and sustainable food source, with favorable medicinal properties (e.g., antitumor, immunomodulatory) (6). Mushroom-forming fungi share a single origin of fruiting body formation that probably dates to the most recent common ancestor of the Agaricomycetes, Dacrymycetes, and Tremellomycetes (2).Fruiting body development in mushroom-forming fungi has been subject to surprisingly few studies (see, e.g., refs. 7-10), result...

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“…Although transcription factor families were usually not conserved, we found 5 TF families that contained developmentally regulated genes from 5 or 6 species (Supplementary Table 10). These included C2H2-type zinc fingers (including c2h2 of Schizophyllum 14,51 ), Zn(2)-C6 fungal-type and homeobox TFs (containing hom1 of Schizophyllum 14,51 ). Two clusters of C2H2 and homeobox TFs showed expression peaks in stipes of Coprinopsis, Lentinus, Armillaria and Rickenella , confirming previous reports of Hom1 expression in Coprinopsis 52 and Schizophyllum 51,53 .…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Krizsán

Almási

Merényi

et al. 2018

Preprint

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We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall (FCW) remodeling, targeted protein degradation, signal transduction, adhesion and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, protein kinases and cadherin-like proteins, showed massive expansions in Agaricomycetes, with many convergently expanded in multicellular plants and/or animals too, reflecting broad genetic convergence among independently evolved complex multicellular lineages. This study provides a novel entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms.

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The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

Cited by 43 publications

References 23 publications

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

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