The transcription regulator AllR senses both allantoin and glyoxylate and controls a set of genes for degradation and reutilization of purines

Hasegawa, Akiko; Ogasawara, Hiroshi; Kori, Ayako; Teramoto, Jun; Ishihama, Akira

doi:10.1099/mic.0.2008/020016-0

Cited by 32 publications

(31 citation statements)

References 24 publications

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“…Similarly, glyoxylate also seems to decrease the AllR binding to its cognate sequence; however, this effect was only observed for the allR-allB promoter region. Taken together, these results differ from studies performed in E. coli, where allantoin and glyoxylate have been proposed as effector molecules of AllR protein but act as a corepressor and a coactivator of the allantoin pathway, respectively (32).…”

Section: Discussioncontrasting

confidence: 54%

AllR Controls the Expression of Streptomyces coelicolor Allantoin Pathway Genes

Navone

Macagno

Licona-Cassani

et al. 2015

Appl Environ Microbiol

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cStreptomyces species are native inhabitants of soil, a natural environment where nutrients can be scarce and competition fierce. They have evolved ways to metabolize unusual nutrients, such as purines and its derivatives, which are highly abundant in soil. Catabolism of these uncommon carbon and nitrogen sources needs to be tightly regulated in response to nutrient availability and environmental stimulus. Recently, the allantoin degradation pathway was characterized in Streptomyces coelicolor. However, there are questions that remained unanswered, particularly regarding pathway regulation. Here, using a combination of proteomics and genetic approaches, we identified the negative regulator of the allantoin pathway, AllR. In vitro studies confirmed that AllR binds to the promoter regions of allantoin catabolic genes and determined the AllR DNA binding motif. In addition, effector studies showed that allantoic acid, and glyoxylate, to a lesser extent, inhibit the binding of AllR to the DNA. Inactivation of AllR repressor leads to the constitutive expression of the AllR regulated genes and intriguingly impairs actinorhodin and undecylprodigiosin production. Genetics and proteomics analysis revealed that among all genes from the allantoin pathway that are upregulated in the allR mutant, the hyi gene encoding a hydroxypyruvate isomerase (Hyi) is responsible of the impairment of antibiotic production.

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Section: Discussioncontrasting

confidence: 54%

AllR Controls the Expression of Streptomyces coelicolor Allantoin Pathway Genes

Navone

Macagno

Licona-Cassani

et al. 2015

Appl Environ Microbiol

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“…The TF PurR plays a key role in the regulation of the upstream pathways, whilst AllR plays a major role in the regulation of the downstream pathway (Rintoul et al, 2002;Walker et al, 2006;Hasegawa et al, 2008). The non-enzymic pathway is known as the intermediate pathway for the conversion of uric acid to allantoin, but the enzymes involved in these reaction steps remain unidentified (Xi et al, 2000).…”

Section: Discussion Regulation Targets Of Yedvwmentioning

confidence: 99%

Cooperative regulation of the common target genes between H2O2-sensing YedVW and Cu2+-sensing CusSR in Escherichia coli

et al. 2015

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YedVW is one of the uncharacterized two-component systems (TCSs) of Escherichia coli. In order to identify the regulation targets of YedVW, we performed genomic SELEX (systematic evolution of ligands by exponential enrichment) screening using phosphorylated YedW and an E. coli DNA library, and identified YedW-binding sites within three intergenic spacers, yedW-hiuH, cyoA-ampG and cusR-cusC, along the E. coli genome. Using a reporter assay system, we found that transcription of hiuH, encoding 5-hydroxyisourate hydrolase, was induced at high concentrations of either Cu 2+ or H 2 O 2 . Cu 2+ -dependent expression of hiuH was observed in the yedWV knockout mutant, but was reduced markedly in the cusRS-null mutant. However, H 2 O 2 -induced hiuH expression was observed in the cusRS-null mutant, but not in the yedWV-null mutant. Gel mobility shift and DNase I footprinting analyses showed binding of both YedW and CusR to essentially the same sequence within the hiuH promoter region. Taken together, we concluded that YedVW and CusSR formed a unique cooperative TCS pair by recognizing and regulating the same targets, but under different environmental conditions -YedVW played a role in H 2 O 2 response regulation, whilst CusSR played a role in Cu 2+ response regulation.

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“…Interaction with specific molecules can positively or negatively modify the affinity of the protein for its target DNA (11,14,17,41,42,44,56). We screened for small molecules that affect transcription from a BaaR-regulated reporter plasmid.…”

Section: ε-Aminocaproic Acid Derepresses Transcription From a Baar-rementioning

confidence: 99%

“…Gene bab2_0215, which encodes a predicted IclR-family transcriptional regulator, was identified as having significantly reduced expression in a σ E1 deletion strain (∆rpoE1) (4). IclR proteins are conserved across bacteria and archaea and have been reported to control the transcription of genes involved in a range of processes (11)(12)(13), including carbon catabolism (14)(15)(16)(17)(18)(19)(20), biofilm formation (21), quorum sensing (22,23), virulence (24)(25)(26), stress response (27), antibiotic resistance (28,29), amino acid and secondary metabolite biosynthesis (30)(31)(32), motility (33), and sporulation (34,35). However, the functional roles of IclR-family regulators in Brucella spp.…”

Section: Introductionmentioning

confidence: 99%