The transcription factor TaLAX1 interacts with Q to antagonistically regulate grain threshability and spike morphogenesis in bread wheat

He, Guanhua; Zhang, Yunwei; Liu, Pan; Jing, Yexing; Zhang, Lichao; Zhu, Yingfang; Kong, Xiuying; Zhao, Huixian; Zhou, Yun; Sun, Jiaqiang

doi:10.1111/nph.17235

Cited by 19 publications

(9 citation statements)

References 57 publications

(87 reference statements)

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“…The recruitment of TaHDA1/TaLHP1 by WFZP may potentially consolidate the chromatin compactness in TaBA1 gene locus, leading to its transcriptional repression. Notably, our current evidence could not rule out the possibility that, in addition to TaBA1 , some other spikelet formation genes might be simultaneously repressed by WFZP and functionally cooperate with TaBA1 to facilitate wheat spike branching, as a recently published work revealed that the in vivo manipulation of TaBA1 (also named TaLAX1 ) seems to be insufficient to simulate the SS spike morphology (He et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat

Zhang

Yuan

et al. 2021

New Phytologist

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Grain yield in bread wheat (Triticum aestivum L.) is largely determined by inflorescence architecture. Zang734 is an endemic Tibetan wheat variety that exhibits a rare triple spikelet (TRS) phenotype with significantly increased spikelet/floret number per spike. However, the molecular basis underlying this specific spike morphology is completely unknown.Through map-based cloning, the causal genes for TRS trait in Zang734 were isolated. Furthermore, using CRISPR/Cas9-based gene mutation, transcriptome sequencing and proteinprotein interaction, the downstream signalling networks related to spikelet formation and awn elongation were defined.Results showed that the null mutation in WFZP-A together with deletion of WFZP-D led to the TRS trait in Zang734. More interestingly, WFZP plays a dual role in simultaneously repressing spikelet formation gene TaBA1 and activating awn development genes, basically through the recruitments of chromatin remodelling elements and the Mediator complex.Our findings provide insights into the molecular bases by which WFZP suppresses spikelet formation but promotes awn elongation and, more importantly, define WFZP-D as a favourable gene for high-yield crop breeding.

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Section: Discussionmentioning

confidence: 99%

FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat

Zhang

Yuan

et al. 2021

New Phytologist

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“…Recently performed yeast two-hybrid assays identified a bHLH family TF, TaLAX1, that can interact with the Q protein to oppositely regulate wheat domestication traits. Moreover, TaLAX1 and Q can antagonistically regulate the transcription of the lignin biosynthesis-related genes TaKNAT7 and TaPAL1 , which were shown to be differentially expressed in previous transcriptome data ( He et al, 2021 ).…”

Section: Introductionmentioning

confidence: 85%

Identification of the Q Gene Playing a Role in Spike Morphology Variation in Wheat Mutants and Its Regulatory Network

et al. 2022

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The wheat AP2 family gene Q controls domestication traits, including spike morphology and threshability, which are critical for the widespread cultivation and yield improvement of wheat. Although many studies have investigated the molecular mechanisms of the Q gene, its direct target genes, especially those controlling spike morphology, are not clear, and its regulatory pathways are not well established. In this study, we conducted gene mapping of a wheat speltoid spike mutant and found that a new allele of the Q gene with protein truncation played a role in spike morphology variation in the mutant. Dynamic expression levels of the Q gene throughout the spike development process suggested that the transcript abundances of the mutant were decreased at the W6 and W7 scales compared to those of the WT. We identified several mutation sites on the Q gene and showed that mutations in different domains resulted in distinct phenotypes. In addition, we found that the Q gene produced three transcripts via alternative splicing and that they exhibited differential expression patterns in nodes, internodes, flag leaves, and spikes. Finally, we identified several target genes directly downstream of Q, including TaGRF1-2D and TaMGD-6B, and proposed a possible regulatory network. This study uncovered the target genes of Q, and the results can help to clarify the mechanism of wheat spike morphology and thereby improve wheat grain yield.

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“…Then, 3 g stem base from each sample was harvested at the four-leaf stage and crosslinked in 1% formaldehyde at room temperature for 10 min, followed by neutralization with 0.25 M glycine. Chromatin immunoprecipitation (ChIP) assays were performed as previously described with modifications (Gendrel et al, 2005;He et al, 2021). ChIP assays were separately performed with or without the anti-Flag antibody.…”

Section: Chromatin Immunoprecipitation Assaysmentioning

confidence: 99%

“…2a). Given that bHLH transcription factors usually perform their functions by forming homodimers or heterodimers (Bu et al, 2011;Seo et al, 2011;Lyu et al, 2019;He et al, 2021), we wondered whether the transcription factor TaPIL1 can form homodimers. LCI assays in N. benthamiana leaves showed that TaPIL1 could indeed form a homodimer in plant cells (Fig.…”

Section: Tapil1 Activates Tatb1 Transcriptionmentioning

confidence: 99%

PIL transcription factors directly interact with SPLs and repress tillering/branching in plants

Zhang

et al. 2021

New Phytologist

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Tillering is an important parameter of plant architecture in cereal crops. In this study, we identified the PHYTOCHROME-INTERACTING FACTOR-LIKE (PIL) family transcription factors as new repressors of tillering in cereal crops.Using biochemical and genetic approaches, we explore the roles of TaPIL1 in regulating wheat plant architecture. We found that the PIL protein TaPIL1 controls tiller number in wheat.Overexpression of TaPIL1 reduces wheat tiller number; additionally, overexpression of TaPIL1-SUPERMAN repression domain increases wheat tiller number. Furthermore, we show that TaPIL1 activates the transcriptional expression of wheat TEOSINTE BRANCHED1 (TaTB1); moreover, TaPIL1 physically interacts with wheat SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (TaSPL)3/17, which are activators of TaTB1 transcription. In rice, overexpression and loss-of-function mutations of OsPIL11 reduce or increase tiller number by regulating the expression of OsTB1. In Arabidopsis, we demonstrate that PHYTOCHROME-INTERACTING FACTOR 4 interacts with SPL9 to inhibit shoot branching.This study reveals that PIL family transcription factors directly interact with SPLs and play an important role in repressing tillering/branching in plants.

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The transcription factor TaLAX1 interacts with Q to antagonistically regulate grain threshability and spike morphogenesis in bread wheat

Cited by 19 publications

References 57 publications

FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat

FRIZZY PANICLE defines a regulatory hub for simultaneously controlling spikelet formation and awn elongation in bread wheat

Identification of the Q Gene Playing a Role in Spike Morphology Variation in Wheat Mutants and Its Regulatory Network

PIL transcription factors directly interact with SPLs and repress tillering/branching in plants

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