2014
DOI: 10.7554/elife.02224
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The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways

Abstract: The neural fate commitment of pluripotent stem cells requires the repression of extrinsic inhibitory signals and the activation of intrinsic positive transcription factors. However, how these two events are integrated to ensure appropriate neural conversion remains unclear. In this study, we showed that Pou3f1 is essential for the neural differentiation of mouse embryonic stem cells (ESCs), specifically during the transition from epiblast stem cells (EpiSCs) to neural progenitor cells (NPCs). Chimeric analysis… Show more

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Cited by 228 publications
(91 citation statements)
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“…Whole-mount in Situ Hybridization-Whole-mount in situ hybridizations were performed as described previously (69). The probes for Oct4, Nanog, and Brachyury were PCR-amplified from mouse cDNA.…”
Section: Crispr/cas Mediates Loss Of Function Of Genes In Episcs-mentioning
confidence: 99%
“…Whole-mount in Situ Hybridization-Whole-mount in situ hybridizations were performed as described previously (69). The probes for Oct4, Nanog, and Brachyury were PCR-amplified from mouse cDNA.…”
Section: Crispr/cas Mediates Loss Of Function Of Genes In Episcs-mentioning
confidence: 99%
“…Similarly, Smad-interacting protein 1 (Sip1; also known as Zeb2) (Chng et al, 2010) and Smad7 (Ozair et al, 2013) were found to act as key intrinsic neural inducers from PSCs, by directly inhibiting the downstream effectors of BMP and Activin signaling. Oct6 (also known as Pou3f1) is another positive inducer of neural fate identified in PSC models, which acts through repression of downstream targets of BMP and Wnt signaling (Zhu et al, 2014).…”
Section: Neural Induction From Pluripotent Stem Cellsmentioning
confidence: 99%
“…Chimeric embryos analysis was performed as described previously 45 . Briefly, GFP-labelled WT mESCs (HDAC1 lox/lox ) and HDAC1 KO mESCs (HDAC1 D/D ) were injected into E2.5 mouse blastocysts, respectively, and then transferred into the uteri of day 2.5 pseudopregnant female mice.…”
Section: Methodsmentioning
confidence: 99%
“…For gene knockdown in mESCs, the corresponding short hairpin RNAs (shRNAs) and control shRNA against Luciferase gene were cloned into the pLKO.1 constructs (Addgene). For the overexpression of HDAC1, the complementary DNA was inserted into pCDH-EF1-Puro vector (Systems Biosciences), the production and infection of lentivirus supernatant was performed as described previously 45 . Briefly, after infection of lentivirus for 24 h, stable mESCs were selected by puromycin (2 mg ml À 1 , Sigma).…”
Section: Methodsmentioning
confidence: 99%
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