The Transcription Factor MafB Regulates the Susceptibility of Bactrocera dorsalis to Abamectin via GSTz2

Tang, Guanghui; Xiong, Ying; Liu, Yi; Song, Zhong-Hao; Yang, Yang; Shen, Guangmao; Wang, Jinjun; Jiang, Hong‐Bo

doi:10.3389/fphys.2019.01068

Cited by 13 publications

(10 citation statements)

References 40 publications

(42 reference statements)

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“…EST and GST are involved in resistance to avermectins. Increased GST and EST levels were observed in arthropod lines that demonstrated resistance to this insecticide [62][63][64] , and inhibition of the detoxification enzymes led to an increase in susceptibility to abamectin 51,[65][66][67] . In particular, direct silencing of GSTz2 expression increased the susceptibility of the fruit fly Bactrocera dorsalis to abamectin 67 .…”

Section: Discussionmentioning

confidence: 99%

“…Increased GST and EST levels were observed in arthropod lines that demonstrated resistance to this insecticide [62][63][64] , and inhibition of the detoxification enzymes led to an increase in susceptibility to abamectin 51,[65][66][67] . In particular, direct silencing of GSTz2 expression increased the susceptibility of the fruit fly Bactrocera dorsalis to abamectin 67 . In Colorado potato beetle larvae, inhibition of esterases by S,S,S-tributyl phosphorotrithioate led to a fivefold increase in susceptibility to abamectin, and inhibition of GST by diethyl maleate caused a twofold increase in susceptibility to this insecticide 68 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Kryukov

Rotskaya

Yaroslavtseva

et al. 2021

Sci Rep

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Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Kryukov

Rotskaya

Yaroslavtseva

et al. 2021

Sci Rep

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“…GST and EST are used by insects to inactivate toxic products formed by insecticide-induced toxicoses (DeSilva et al, 1997; Boyer et al, 2006; Aponte et al, 2013) as well as under mycosis (Dubovskiy et al, 2012). Especially Tang et al (2019) showed that up-regulation of GSTz2 decreased the susceptibility of tephritid fruit fly Bactrocera dorsalis (Hendel) to abamectin. Moreover, GST may participate in inactivation of fungal secondary metabolites (Loutelier, Cherton & Lange, 1994) and reactive oxygen species (Sherratt & Hayes, 2002).…”

Section: Discussionmentioning

confidence: 99%

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Noskov

Polenogova

Yaroslavtseva

et al. 2019

PeerJ

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Combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect of the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber and avermectins on the survival and physiological parameters of Aedes aegypti (Linnaeus, 1762) larvae (dopamine concentration, glutathione S-transferase (GST), nonspecific esterases (EST), acid proteases, lysozyme-like, phenoloxidase (PO) activities) was studied. It is shown that the combination of these agents leads to a synergistic effect on mosquito mortality. Colonization of Ae. aegypti larvae by hyphal bodies following water inoculation with conidia is shown for the first time. The larvae affected by fungi are characterized by a decrease in PO and dopamine levels. In the initial stages of toxicosis and/or fungal infection (12 h posttreatment), increases in the activity of insect detoxifying enzymes (GST and EST) and acid proteases are observed after monotreatments, and these increases are suppressed after combined treatment with the fungus and avermectins. Lysozyme-like activity is also most strongly suppressed under combined treatment with the fungus and avermectins in the early stages posttreatment (12 h). Forty-eight hours posttreatment, we observe increases in GST, EST, acid proteases, and lysozyme-like activities under the influence of the fungus and/or avermectins. The larvae affected by avermectins accumulate lower levels of conidia than avermectin-free larvae. On the other hand, a burst of bacterial CFUs is observed under treatment with both the fungus and avermectins. We suggest that disturbance of the responses of the immune and detoxifying systems under the combined treatment and the development of opportunistic bacteria may be among the causes of the synergistic effect.

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“…Gene activation in higher eukaryotes requires the concerted action of TFs, which have received extensive focus for biological control 38,39 . Also, the knowledge gained from understanding the functions of TFs can be applied to tackle the challenge of pesticide resistance 40,41 . As a result, by enhancing its expression, E74A can serve as an important potential target for improving the biological effect of NPV in pest control.…”

Section: Discussionmentioning

confidence: 99%

“…38,39 Also, the knowledge gained from understanding the functions of TFs can be applied to tackle the challenge of pesticide resistance. 40,41 As a result, by enhancing its expression, E74A can serve as an important potential target for improving the biological effect of NPV in pest control. This was demonstrated with the expression experiment in S. litura (Fig.…”

Section: Discussionmentioning

confidence: 99%

Bombyx mori transcription factor, E74A, beneficially affects BmNPV infection through direct interaction

Wang

Cheng

et al. 2022

Pest Management Science

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BACKGROUND: Nucleopolyhedrovirus (NPV), one of the baculoviruses, is a promising biopesticide for pest control. Lepidopteran account for 70% of pests, therefore investigation on highly conserved genes associated with viral infections in the lepidopteran model, the silkworm, will serve as a valuable reference for improving the effectiveness of pest management. BmE74A is a member of the erythroblast transformation-specific (ETS) family of transcription factors in Bombyx mori, which we previously found to be highly conserved and closely associated with BmNPV. This study aimed to elucidate the role of BmE74A in viral infection.RESULTS: A significantly high expression of BmE74A in eggs indicated its important role in embryonic development, as did relatively high expressions in the hemolymph and midgut. Significant differences in BmE74A expression in different resistant strains after BmNPV infection suggested its involvement as a response to viral infection. Moreover, RNA interference (RNAi) and overexpression experiments confirmed the important role of BmE74A in promoting viral infection. BmNPV infection was significantly suppressed and enhanced by BmE74A knockdown and overexpression, respectively. Besides, BmE74A was found to regulate the expression of BmMdm2 and Bmp53. Furthermore, the binding of ETS, the functional domain of BmE74A, to occlusion-derived virus proteins was confirmed by far-western blotting, and four viral proteins that may interact with ETS proteins were identified by mass spectrometry. Similarly, a homolog of BmE74A in Spodoptera litura was also found to be involved in larval susceptibility to BmNPV. CONCLUSION: BmE74A promotes BmNPV proliferation by directly interacting with the virus, which may be related to the suppression of the p53 pathway.

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The Transcription Factor MafB Regulates the Susceptibility of Bactrocera dorsalis to Abamectin via GSTz2

Cited by 13 publications

References 40 publications

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Bombyx mori transcription factor, E74A, beneficially affects BmNPV infection through direct interaction

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