The transcription factor FgMed1 is involved in early conidiogenesis and DON biosynthesis in the plant pathogenic fungus Fusarium graminearum

Fan, Guang; Zhang, Kai; Zhang, Jing; Yang, Jie; Yang, Xiaoshuang; Hu, Yanpei; Huang, Jiawei; Zhu, Yangyan; Hu, Hong; Wang, Baohua; Shim, Won‐Bo; Lu, Guodong

doi:10.1007/s00253-019-09872-2

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…The homologs of FocmedA(a) have been identified in some other filamentous fungi. In F. graminearum, loss of the medA homolog gene FgMed1 affects the formation of phialide, but the FgMed1 deletion mutant can still produce conidia directly from abnormal conidiophores [66]. In A. nidulans, loss of medA causes proliferation of branching chains of metulae and delays phialide formation and conidia brlA is the initial CRP gene in A. nidulans, but its homolog gene was not identified in Foc; we wondered which gene in Foc plays the role of brlA in A. nidulans (especially in the activation of abaA).…”

Section: Discussionmentioning

confidence: 99%

A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense

Lu,

Deng,

Lin

et al. 2023

JoF

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The conidia produced by Fusarium oxysporum f. sp. cubense (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of asexual sporogenesis has not been clearly understood in Foc. Herein, we identified and functionally characterized thirteen (13) putative sporulation-responsive genes in Foc, namely FocmedA(a), FocmedA(b), abaA-L, FocflbA, FocflbB, FocflbC, FocflbD, FocstuA, FocveA, FocvelB, wetA-L, FocfluG and Foclae1. We demonstrated that FocmedA(a), abaA-L, wetA-L, FocflbA, FocflbD, FocstuA, FocveA and Foclae1 mediate conidiophore formation, whereas FocmedA(a) and abaA-L are important for phialide formation and conidiophore formation. The expression level of abaA-L was significantly decreased in the ΔFocmedA(a) mutant, and yeast one-hybrid and ChIP-qPCR analyses further confirmed that FocMedA(a) could bind to the promoter of abaA-L during micro- and macroconidiation. Moreover, the transcript abundance of the wetA-L gene was significantly reduced in the ΔabaA-L mutant, and it not only was found to function as an activator of micro- and macroconidium formation but also served as a repressor of chlamydospore production. In addition, the deletions of FocflbB, FocflbC, FocstuA and Foclae1 resulted in increased chlamydosporulation, whereas FocflbD and FocvelB gene deletions reduced chlamydosporulation. Furthermore, FocflbC, FocflbD, Foclae1 and FocmedA(a) were found to be important regulators for pathogenicity and fusaric acid synthesis in Foc. The present study therefore advances our understanding of the regulation pathways of the asexual development and functional interdependence of sporulation-responsive genes in Foc.

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Section: Discussionmentioning

confidence: 99%

A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense

Lu,

Deng,

Lin

et al. 2023

JoF

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“…The RNA sequencing of deletion mutants of mycotoxigenic fungi enabled the elucidation of some regulatory mechanisms underlying mycotoxin biosynthesis. Recent works have shown that transcription factors involved in fungal conidial development and sclerotial production have a role in the pathogenesis/virulence and mycotoxin biosynthesis [40,41]. In addition, studies on deletion mutants may support the role of global transcription factors involved in signal transduction, leading to the production of mycotoxins as well as the possible activity of cluster-specific transcription factors in influencing the expression of genes in a different secondary metabolic pathway [42,43].…”

Section: Transcriptomicsmentioning

confidence: 99%

Current Approaches for Advancement in Understanding the Molecular Mechanisms of Mycotoxin Biosynthesis

Gallo

Perrone

2021

IJMS

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Filamentous fungi are able to synthesise a remarkable range of secondary metabolites, which play various key roles in the interaction between fungi and the rest of the biosphere, determining their ecological fitness. Many of them can have a beneficial activity to be exploited, as well as negative impact on human and animal health, as in the case of mycotoxins contaminating large quantities of food, feed, and agricultural products worldwide and posing serious health and economic risks. The elucidation of the molecular aspects of mycotoxin biosynthesis has been greatly sped up over the past decade due to the advent of next-generation sequencing technologies, which greatly reduced the cost of genome sequencing and related omic analyses. Here, we briefly highlight the recent progress in the use and integration of omic approaches for the study of mycotoxins biosynthesis. Particular attention has been paid to genomics and transcriptomic approaches for the identification and characterisation of biosynthetic gene clusters of mycotoxins and the understanding of the regulatory pathways activated in response to physiological and environmental factors leading to their production. The latest innovations in genome-editing technology have also provided a more powerful tool for the complete explanation of regulatory and biosynthesis pathways. Finally, we address the crucial issue of the interpretation of the combined omics data on the biology of the mycotoxigenic fungi. They are rapidly expanding and require the development of resources for more efficient integration, as well as the completeness and the availability of intertwined data for the research community.

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A feedback regulation of FgHtf1-FgCon7 loop in conidiogenesis and development of Fusarium graminearum

Chen,

Li,

Abubakar

et al. 2024

International Journal of Biological Macromolecules

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The transcription factor FgMed1 is involved in early conidiogenesis and DON biosynthesis in the plant pathogenic fungus Fusarium graminearum

Cited by 5 publications

References 54 publications

A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense

A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense

Current Approaches for Advancement in Understanding the Molecular Mechanisms of Mycotoxin Biosynthesis

A feedback regulation of FgHtf1-FgCon7 loop in conidiogenesis and development of Fusarium graminearum

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