The transcription factor CsbHLH18 of sweet orange functions in modulation of cold tolerance and homeostasis of reactive oxygen species by regulating the antioxidant gene

Geng, Jingjing; Liu, Jihong

doi:10.1093/jxb/ery065

Cited by 85 publications

(64 citation statements)

References 64 publications

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“…It is well established that antioxidant enzymes, such as superoxide dismutase (SOD), POD and catalase (CAT), play a crucial and predominant role in eliminating the ROS produced under abiotic stresses (Gill and Tuteja, ). Increasing evidence demonstrated that a high antioxidant capacity to scavenge the ROS is linked to increased tolerance to environmental stresses (Geng and Liu, ; Huang et al ., ). Interestingly, we found that several POD‐encoding genes were considerably up‐regulated in the PtrERF109 ‐overexpressing line.…”

Section: Discussionmentioning

confidence: 99%

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Wang

Dai

et al. 2019

Plant Biotechnology Journal

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Summary Ethylene‐responsive factors ( ERF s) have been revealed to play essential roles in a variety of physiological and biological processes in higher plants. However, functions and regulatory pathways of most ERF s in cold stress remain largely unclear. Here, we identified Ptr ERF 109 of trifoliate orange ( Poncirus trifoliata (L.) Raf.) and deciphered its role in cold tolerance. Ptr ERF 109 was drastically up‐regulated by cold, ethylene and dehydration, but repressed by salt. Ptr ERF 109 was localized in the nucleus and displayed transcriptional activity, and the C terminus is required for the activation. Overexpression of Ptr ERF 109 conferred enhanced cold tolerance in transgenic tobacco and lemon plants, whereas VIGS (virus‐induced gene silencing)‐mediated suppression of Ptr ERF 109 in trifoliate orange led to increased cold susceptibility. Ptr ERF 109 overexpression caused extensive transcriptional reprogramming of several suites of stress‐responsive genes. Prx1 encoding class III peroxidase ( POD ) was one of the antioxidant genes exhibiting the greatest induction. Ptr ERF 109 was shown to directly bind to the promoter of PtrPrx1 (trifoliate orange Prx1 homologue) and positively activated its expression. In addition, the Ptr ERF 109 ‐overexpressing plants exhibited significantly higher POD activity and accumulated dramatically less H 2 O 2 and were more tolerant to oxidative stress, whereas the VIGS plants exhibited opposite trends, in comparison with wild type. Taken together, these results indicate that Ptr ERF 109 as a positive regulator contributes to imparting cold tolerance by, at least partly, directly regulating the POD ‐encoding gene to maintain a robust antioxidant capacity for effectively scavenging the reactive oxygen species. Our findings gain insight into better understanding of transcriptional regulation of antioxidant genes in response to cold stress.

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Section: Discussionmentioning

confidence: 99%

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Wang

Dai

et al. 2019

Plant Biotechnology Journal

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“…For analyses of MDA levels, peroxidase (POD) and superoxide dismutase (SOD) activity, H 2 O 2 , anti‐O 2 •− capacity, sucrose and glucose content, 0.2 g of leaf samples were homogenized in 2 mL cold extraction buffer (0.1 m phosphate buffer, pH 7.0). After centrifugation for 10 min at 8000 rpm, the supernatants were used for measurements of the parameters using corresponding assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions and as described previously (Geng and Liu, ; Gong et al ., ). MDA, POD, SOD, H 2 O 2 , anti‐O 2 •− and sucrose were calculated based on total protein content of the samples (nmol/mg protein for MDA, unit (U)/mg protein for POD and SOD, mmol/g protein for H 2 O 2 , U/g protein for anti‐O 2 •− , μmol/mg protein for sucrose), whereas glucose was calculated in mmol/L.…”

Section: Methodsmentioning

confidence: 99%

Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata

Wei

Wang

Xie

et al. 2019

Plant Biotechnology Journal

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105

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Summary Tetraploids have been reported to exhibit increased stress tolerance, but the underlying molecular and physiological mechanisms remain poorly understood. In this study, autotetraploid plants were identified by screening natural seedlings of trifoliate orange ( Poncirus trifoliata ). The tetraploids exhibited different morphology and displayed significantly enhanced drought and dehydration tolerance in comparison with the diploid progenitor. Transcriptome analysis indicated that a number of stress‐responsive genes and pathways were differentially influenced and enriched in the tetraploids, in particular those coding for enzymes related to antioxidant process and sugar metabolism. Transcript levels and activities of antioxidant enzymes (peroxidase and superoxide dismutase) and sucrose‐hydrolysing enzyme (vacuolar invertase) were increased in the tetraploids upon exposure to the drought, concomitant with greater levels of glucose but lower level of reactive oxygen species ( ROS ). These data indicate that the tetraploids might undergo extensive transcriptome reprogramming of genes involved in ROS scavenging and sugar metabolism, which contributes, synergistically or independently, to the enhanced stress tolerance of the tetraploid. Our results reveal that the tetraploids take priority over the diploid for stress tolerance by maintaining a more robust system of ROS detoxification and osmotic adjustment via elevating antioxidant capacity and sugar accumulation in comparison with the diploid counterpart.

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“…Transcription factors (TFs) play key roles in the stress regulation network and signal pathways in plants. Basic/helix-loop-helix (bHLH) TFs are the second largest TFs family in plants and have been identified in many species [6,[18][19][20][21][22][23][24][25]. However, the bHLH TF family has not previously been reported in papaya (Carica papaya L.).…”

Section: Discussionmentioning

confidence: 99%

“…The detailed information on these CpbHLHs, including protein ID, locus ID, opening reading frame (ORF) lengths, amino acid sequences/lengths, molecular weight, isoelectric point and exon/intron numbers, are listed in Additional file 1. In previous studies, 129/132, 188, 159, 147, 124, 95, 94 and 56 bHLH genes were identified in peanut [18], apple [19], tomato [20], Arabidopsis [6], potato [21], peach [22], grapes [23] and sweet orange [24], respectively. Compared with the above dicotyledonous plants, the density of bHLHs superfamily genes in papaya genome was approximately 0.26%, which is lower than the density of peanut [18], apple [19], tomato [20], arabidopsis [6], potato [21] and sweet orange [24], and similar to peach [22] and wine grapes [23] (Table 1).…”

Section: Identification and Characterization Of Cpbhlhsmentioning

confidence: 93%

“…Phylogenetic analyses of some atypical bHLH proteins have even extended that number to 32 [5]. With the availability of genome sequence data and the rapid development of molecular biology, increasing numbers of bHLH subfamily genes have been identified and characterized in a wide range of plant species, including Arabidopsis [6], peanut [18], apple [19], tomato [20], potato [21], peach [22], grapes [23], sweet orange [24], and bamboo [25]. The results from these studies have shown that bHLH TFs have versatile biological functions, such as regulating light morphogenesis [26,27], hormone signals [28,29], the developmental of root [30] and anther [31], regulating epidermal cell fate determination [32], participating in various biotic and abiotic stress responses [21,33,34], etc.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of basic helix-loop-helix (bHLH) transcription factors in papaya (Carica papaya L.)

Yang¹,

Kuang²,

Zhou³

et al. 2019

Preprint

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Background : As a superfamily of transcription factors (TFs), the basic helix-loop-helix (bHLH) proteins have been identified and functionally characterized in many plants. However, no comprehensive analysis of the bHLH family in papaya ( Carica papaya L. ) has been reported previously. Results: In this study, a total of 73 CpbHLH genes were found in papaya, and these genes were classified into 18 subfamilies based on phylogenetic analysis, with one orphans. Almost all of the CpbHLH in the same subfamily shared similar gene structures and protein motifs according to an analysis of exon/intron organizations and motif compositions. The number of exons in CpbHLH genes varied from 1 to 11 with an average of 5. The amino acid sequences of the bHLH domains were quite conservative, especially Leu-27 and Leu-63. Promoter cis -element analysis revealed that most of the CpbHLH genes contained cis -elements that can respond to various biotic/abiotic stress-related events. Gene ontology (GO) analysis revealed that Cp bHLH mainly functions in protein dimerization activity and DNA-binding, and most Cp bHLH proteins were predicted to localize in the nucleus. Abiotic stress treatment and quantitative real-time PCR (qRT-PCR) revealed some predicted CpbHLH genes that might be responsible for abiotic stress responses in papaya. Conclusions : A total of 73 bHLH transcription factors were identified from papaya, and their gene structures, conserved domains, sequence features, phylogenetic relationship, promoter cis -element, GO annotation and gene expression profiles responsible for abiotic stress were investigated. Our findings lay a foundation for further evolutionary and functional elucidation of Cp bHLHs. Keywords : papaya, genome-wide analysis, bHLH transcription factors, abiotic stress

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The transcription factor CsbHLH18 of sweet orange functions in modulation of cold tolerance and homeostasis of reactive oxygen species by regulating the antioxidant gene

Abstract: CsbHLH18, a bHLH transcription factor of Citrus sinensis, functions as a positive regulator of cold tolerance due to promoted ROS scavenging, and directly targets a POD gene.

Cited by 85 publications

References 64 publications

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata

Genome-wide analysis of basic helix-loop-helix (bHLH) transcription factors in papaya (Carica papaya L.)

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