The Trace and Subgrouping of Lymphocyte Activation by Dynamic Fluorescence Intensity and Polarization Measurements

Sunray, Merav; Kaufman, Menachem; Zurgil, Naomi; Deutsch, Mordechai

doi:10.1006/bbrc.1999.0304

Cited by 15 publications

(9 citation statements)

References 34 publications

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“…The basic features of the methodology behind its design were published previously (22,23). One of the main ICS features is a cell tray that enables the localization of cells.…”

Section: The Ics Apparatusmentioning

confidence: 99%

Determination of individual cell Michaelis‐Menten constants

et al. 2001

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Background: A novel methodology for the measurement and analysis of apparent K M (Michaelis-Menten constant) and V MAX values of individual cells is suggested. It is based on a mathematical model that considers substrate influx into the cell, its intracellular enzymatic hydrolysis, and the product efflux. The mathematical formulation was approximated linearly in order to analyze intracellular substrate conversion characteristics via Michaelis-Menten theory.

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“…The basic features of the methodology behind its design were published previously (22,23). One of the main ICS features is a cell tray that enables the localization of cells.…”

Section: The Ics Apparatusmentioning

confidence: 99%

Determination of individual cell Michaelis‐Menten constants

et al. 2001

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“…Kinetic and cluster analysis were performed as described previously (18). The source used for calibration of FI was a tritium filled fluorescent tube (␤-light).…”

Section: Calibration Of Cs-s and Kinetic Analysis Of Resultsmentioning

confidence: 99%

Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements

Deutsch

Kaufman

Shapiro³

et al. 2000

Cytometry

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“…All groups showed a decrease in FP values over time, but while the L subgroup quantitatively conserved fluorescence depolarization (FDP), in the other subgroups (H and M) the FDP decreased or even changed sign. The distinction between these three groups is therefore important in the prediction of FP changes following stimuli (Sunray et al 1999).…”

Section: Monitoring Lymphocyte Activationmentioning

confidence: 99%

Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

Harel

Gilburd

Schiffenbauer

et al. 2005

Journal of Immunology Research

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The CellScan apparatus is a laser scanning cytometer enabling repetitive fluorescence intensity (FI) and polarization (FP) measurements in living cells, as a means of monitoring lymphocyte activation. The CellScan may serve as a tool for diagnosis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) as well as other autoimmune diseases by monitoring FP changes in peripheral blood lymphocytes (PBLs) following exposure to autoantigenic stimuli. Changes in FI and FP in atherosclerotic patients' PBLs following exposure to various stimuli have established the role of the immune system in atherosclerotic disease. The CellScan has been evaluated as a diagnostic tool for drug-allergy, based on FP reduction in PBLs following incubation with allergenic drugs. FI and FP changes in cancer cells have been found to be well correlated with the cytotoxic effect of anti-neoplastic drugs. In conclusion, the CellScan has a variety of applications in cell biology, immunology, cancer research and clinical pharmacology.

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The Trace and Subgrouping of Lymphocyte Activation by Dynamic Fluorescence Intensity and Polarization Measurements

Cited by 15 publications

References 34 publications

Determination of individual cell Michaelis‐Menten constants

Determination of individual cell Michaelis‐Menten constants

Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements

Application of a Static Fluorescence‐based Cytometer (the CellScan) in Basic Cytometric Studies, Clinical Pharmacology, Oncology and Clinical Immunology

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