The toxicity study on marine low-temperature lysozyme

Yan, Cui‐Wei; Ding, Boxiao; Xiao-ming, Lan; Guo, Shen-Bo; Xie, Ying; Wang, Chunbo

doi:10.1016/j.fct.2007.09.001

Cited by 9 publications

(7 citation statements)

References 4 publications

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“…The ability of lysozyme‐only to permeabilize S. aureus is likely owing to how the isolates were prepared after culturing and how they were fixed and permeabilized. Isolates were diluted in agarose to enhance signal intensity 16, 27; fixed in mid‐log phase when rRNA numbers were high 1, 13; permeabilized by heat, methanol 6 and lysozyme 12; treated with a relatively high concentration of unbuffered lysozyme 12, 18–20; and finally incubated for an extended period of time 12 at an optimal temperature for lytic activity 21.…”

Section: Discussionmentioning

confidence: 99%

“…The slides were cooled and 10 μl of freshly prepared 15 mg/ml lysozyme 18 in unbuffered MQ water 12, 19, 20 was pipetted to each well. Typically, lysozyme is buffered with Tris–HCl at pH 8.0 2, 3, 5, 6, 13, but for simplicity and to attain a more intense signal 21, we followed reports where buffering was omitted 12, 19, 20. Slides were fitted in 50 ml tubes to prevent evaporation and then placed in a 37°C 21 incubator for 30 min 12.…”

Section: Methodsmentioning

confidence: 99%

“…Typically, lysozyme is buffered with Tris–HCl at pH 8.0 2, 3, 5, 6, 13, but for simplicity and to attain a more intense signal 21, we followed reports where buffering was omitted 12, 19, 20. Slides were fitted in 50 ml tubes to prevent evaporation and then placed in a 37°C 21 incubator for 30 min 12. The lysozyme action was stopped by immersion in absolute methanol for 1 min and then dried on a hotplate 6.…”

Section: Methodsmentioning

confidence: 99%

“…The protocol was identical to the lysozyme‐only treatment, but with the following modifications. Lysostaphin (Sigma, L4402) at 100 μg/ml 3 was added to the lysozyme in MQ water 19 and incubated at 40°C 21, 22 for 3 6 instead of 30 min. As a control, lysozyme–lysostaphin was kept unbuffered, but we observed cell morphology was better preserved if it was buffered at pH 8.0 2, 3, 5, 6, 13.…”

Section: Methodsmentioning

confidence: 99%

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Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

Lawson¹,

Connally²,

Iredell³

et al. 2011

Clinical Laboratory Analysis

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To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lysozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilization to S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1 hr protocol is a useful alternative to conventional FISH.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

Lawson¹,

Connally²,

Iredell³

et al. 2011

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…29 The optimum pH for lysozyme in PBS is around 6.2-7.3 30 with a 70% stability. 31 After 14 days, filtered aliquots (1 mL) withdrawn were cooled in an ice bath, mixed with alkaline potassium ferricyanide solution (4 mL); prepared from 0.25 g potassium ferricyanide complex salt dissolved in sodium carbonate: 0.5M, 500 mL) and quenched in boiling water, for 15 min; following by a rapid cooling at room temperature within 5 min in a ice bath. Optical absorbance of the solution was measured at 420 nm in a UV-VIS spectrophotometer, (Shimadzu 1700, Japan).…”

Section: Sem Xrd and Edx Analysismentioning

confidence: 99%

Biomimetic chitosan–calcium phosphate composites with potential applications as bone substitutes: Preparation and characterization

2011

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A novel biomimetic technique for obtaining chitosan-calcium phosphates (Cs-CP) scaffolds are presented: calcium phosphates are precipitated from its precursors, CaCl(2) and NaH(2) PO(4) on the Cs matrix, under physiological conditions (human body temperature and body fluid pH; 37°C and pH = 7.2, respectively). Materials composition and structure have been confirmed by various techniques: elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM). FTIR and SEM data have shown the arrangement of the calcium phosphates-hydroxyapatite (CP-Hap) onto Cs matrix. In this case the polymer is acting as glue, bonding the calcium phosphates crystals. Behavior in biological simulated fluids (phosphate buffer solution-PBS and PBS-albumin) revealed an important contribution of the chelation between -NH3(+) and Ca(2+) on the scaffold interaction with aqueous mediums; increased quantities of chitosan in composites permit the interaction with human albumin and improve the retention of fluid. The composites are slightly degraded by the lysozyme which facilitates an in vivo degradation control of bone substitutes. Modulus of elasticity is strongly dependent of the ratio chitosan/calcium phosphates and recommends the obtained biomimetic composites as promising materials for a prospective bone application.

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Solar‐Driven Drum‐Type Atmospheric Water Harvester Based on Bio‐Based Gels with Fast Adsorption/Desorption Kinetics

Zhou,

Yan,

Tang

et al. 2024

Advanced Materials

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Sorption‐based atmospheric water harvesting is an attractive technology for exploiting unconventional water sources. A critical challenge is how to facilitate fast and continuous collection of potable water from air. Here, a bio‐based gel (CAL gel), resulting from the integration of a whole biomass‐derived polymer network with lithium chloride is reported. A fast adsorption/desorption kinetics, with a water capture rate of 1.74 kg kg−1 h−1 at 30% relative humidity and a desorption rate of 1.98 kg kg−1 h−1, was simultaneously realized in one piece of CAL gel, because of its strong hygroscopicity, hydrophilic network, abundant water transport channels, photothermal conversion ability, and ∼200‐μm‐thick self‐supporting bulky structure caused by multicomponent synergy. A solar‐driven, drum‐type, tunable, and portable harvester is designed that can harvest atmospheric water within a brief time. Under outdoor conditions, the harvester with CAL gels operates 36 switches (180°) per day realizes a water yield of 8.96 kg kggel−1 (18.87 g kgdevice−1). This portable harvester highlights the potential for fast and scalable atmospheric water harvesting in extreme environments.This article is protected by copyright. All rights reserved

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The toxicity study on marine low-temperature lysozyme

Cited by 9 publications

References 4 publications

Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

Biomimetic chitosan–calcium phosphate composites with potential applications as bone substitutes: Preparation and characterization

Solar‐Driven Drum‐Type Atmospheric Water Harvester Based on Bio‐Based Gels with Fast Adsorption/Desorption Kinetics

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