The toxicity mechanism of different sized iron nanoparticles on human breast cancer (MCF7) cells

Zhang, Yuanxiao; Yu, Hai; Miao, Yuqing; Xiao, Qi; Wang, Xue; Luo, Yane; Fan, Haiming; Yue, Tianli

doi:10.1016/j.foodchem.2020.128263

Cited by 27 publications

(22 citation statements)

References 39 publications

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“…The 9 nm Fe 3 O 4 nanoparticles mediated mitochondria-dependent intracellular ROS generation to induce cellular mitochondrial dysfunction and necrosis, while the 14 nm Fe 3 O 4 nanoparticles led to plasma membrane damage. Luo et al obtained similar results that a suitable size (15.1 nm) of superparamagnetic iron oxide nanoparticles (SPIONs) enhanced the uptake amount into MCF7 cells, leading to the formation of more ROS (Zhang et al, 2020c). Promoted ROS was produced in mitochondria to destroy mitochondria by small size (7.3 nm) SPIONs, while more ROS was yield in plasma to destroy cytomembrane by larger size (15.1, 30.0 nm) SPIONs.…”

Section: Intrinsic Fenton Reaction Of Ferritementioning

confidence: 83%

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

Zhang

et al. 2021

Front. Chem.

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Ferrite nanoparticles have been widely used in the biomedical field (such as magnetic targeting, magnetic resonance imaging, magnetic hyperthermia, etc.) due to their appealing magnetic properties. In tumor acidic microenvironment, ferrite nanoparticles show intrinsic peroxidase-like activities, which can catalyze the Fenton reaction of hydrogen peroxide (H2O2) to produce highly toxic hydroxyl free radicals (•OH), causing the death of tumor cell. Recent progresses in this field have shown that the enzymatic activity of ferrite can be improved via converting external field energy such as alternating magnetic field and near-infrared laser into nanoscale heat to produce more •OH, enhancing the killing effect on tumor cells. On the other hand, combined with other nanomaterials or drugs for cascade reactions, the production of reactive oxygen species (ROS) can also be increased to obtain more efficient cancer therapy. In this review, we will discuss the current status and progress of the application of ferrite nanoparticles in ROS-mediated cancer therapy and try to provide new ideas for this area.

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Section: Intrinsic Fenton Reaction Of Ferritementioning

confidence: 83%

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

Zhang

et al. 2021

Front. Chem.

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“…Excessive iron ions in cells can cause abnormal iron metabolism and oxidative stress, accompanying ROS production [32]. We found that the production of intracellular ROS increased proportionally to the exposure time of NPs, suggesting that the presence of NPs correlates with ROS production in the cells.…”

Section: Discussionmentioning

confidence: 65%

p53 Promotes Ferroptosis in Macrophages Treated with Fe3O4 Nanoparticles

Zhang

Shen

et al. 2021

Preprint

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Background: Fe3O4 nanoparticles (NPs, also known as iron oxide NPs; IONPs) have high biocompatibility and low biotoxicity. They are widely used in the field of biotechnology for targeted delivery, image formation, and photothermal therapy. NP biodistribution is determined by macrophage capture in vivo, and recently, the induction of macrophage polarization into the M1 phenotype by IONPs has become a hot topic in research. Previous research has shown that IONPs can induce ferroptosis of ovarian cancer cells and ischemic cardiomyocytes. In this study, we exposed macrophages to synthesized Fe3O4 NPs (100 nm in diameter) and determined the effects of NPs in inducing cell death by RNA sequencing.Results: We observed that after 48 h exposure to NPs, there was a change in the macrophage phenotype and a reduction in cell viability. Then, we demonstrated that NPs could induce macrophage cell damage by increasing intracellular reactive oxygen species and by repressing the mitochondrial membrane potential. Furthermore, we investigated the underlying mechanisms of ferroptosis of macrophages using RNA sequencing and change in ultrastructural morphology, and found that ferroptosis was caused by the upregulation of p53 expression and inhibition of SLC7A11 expression, as their protein levels after 48 h exposure to Fe3O4 NPs were consistent with erastin-induced ferroptosis. Conclusions: These results provide an insight into the molecular mechanisms underlying ferroptosis induced by Fe3O4 NPs in macrophages and provide a basis for the biotoxicity study of Fe3O4 NPs in vivo.

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“…Apoptosis referred to the orderly and autonomous death of cells controlled by genes in order to maintain a stable internal environment [ 38 ]. Mitochondria were the control centers for cell life activities, including apoptosis regulation along with cell respiratory chain and oxidative phosphorylation.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical drug release designed Au @PDA-PEG-MTX NPs for targeted delivery to breast cancer with combined photothermal-chemotherapy

Cao

et al. 2021

J Nanobiotechnol

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Breast cancer (BC) is the most frequently diagnosed cancer with a low survival rate and one of the major causes of cancer-related death. Methotrexate (MTX) is an anti-tumor drug used in the treatment of BC. Poor dispersion in water and toxic side effects limit its clinical application. Gold nanoparticles (AuNPs), owing to their specific structures and unique biological and physiochemical properties, have emerged as potential vehicles for tumor targeting, bioimaging and cancer therapy. An innovative nano drug-loading system (Au @PDA-PEG-MTX NPs) was prepared for targeted treatment of BC. Au @PDA-PEG-MTX NPs under near infra-red region (NIR) irradiation showed effective photothermal therapy against MDA-MB-231 human BC cells growth in vitro by inducing apoptosis through triggering reactive oxygen species (ROS) overproduction and generating excessive heat. In vivo studies revealed deep penetration ability of Au @PDA-PEG-MTX NPs under NIR irradiation to find application in cancer-targeted fluorescence imaging, and exhibited effective photothermal therapy against BC xenograft growth by inducing apoptosis. Histopathological analysis, cellular uptake, cytotoxicity assay, and apoptosis experiments indicated that Au @PDA-PEG-MTX NPs possessed a good therapeutic effect with high biocompatibility and fewer side effects. This Au NPs drug-loading system achieved specific targeting of MTX to BC cells by surface functionalisation, fluorescence imaging under laser irradiation, combined photothermal-chemotherapy, and pH- and NIR- triggered hierarchical drug release.

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The toxicity mechanism of different sized iron nanoparticles on human breast cancer (MCF7) cells

Cited by 27 publications

References 39 publications

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

p53 Promotes Ferroptosis in Macrophages Treated with Fe3O4 Nanoparticles

Hierarchical drug release designed Au @PDA-PEG-MTX NPs for targeted delivery to breast cancer with combined photothermal-chemotherapy

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