The Toxic Effect of Water-Soluble Particulate Pollutants from Biomass Burning on Alveolar Lung Cells

Abstract

In 2018, 3.8 million premature deaths were attributed to exposure to biomass burning nanoparticles from wood combustion. The objective of this study was to investigate and compare the toxic effect of wood-combustion-related biomass burning nanoparticles from three different combustion stages (i.e., flaming, smoldering, and pyrolysis) on alveolar lung cells, by studying cell proliferation, and structural and behavioral parameters. A549 lung epithelial cells were treated with 31, 62, 125, 250, and 500 µg/mL of water-soluble particulate pollutants from wood burning, and measured by means of real-time cell analysis, cell imaging, and phase imaging microscopy. At low concentrations (31 and 62 µg/mL), all three types of wood burning samples exhibited no toxicity. At 125 µg/mL, they caused decreased cell proliferation compared to the control. Exposure to higher concentrations (250 and 500 µg/mL) killed the cells. Cell physical parameters (area, optical volume, eccentricity, perimeter, and optical thickness) and behavioral parameters (migration, motility, and motility speed) did not change in response to exposure to wood burning materials up to a concentration of 125 µg/mL. Exposure to higher concentrations (250 and 500 µg/mL) changed cell perimeter, optical thickness for smoldering and flaming particles, and led to decreased migration, motility, and motility speed of cells. In conclusion, all three of the combustion water-soluble organic pollutants were identified as equally toxic by real-time cell analysis (RTCA) results. The parameters describing cell structure suggest that pyrolysis particles were slightly less toxic than others.

Comparative Experimental Assessment of Pollutant Emission Behavior in Combustion of Untreated and Thermally Treated Solid Biofuels from Spruce Chips and Rapeseed Straw

“…However, for a fuel, being CO 2 neutral is not necessarily good enough. It also needs to be easily and efficiently utilized and it must not produce excessive emissions of pollutants, such as CO, NOx, and particulate matter, which can by themselves cause damage by contributing to increases in some illnesses [5,6].…”

Cytotoxicity and oxidative stress induced by atmospheric mono-nitrophenols in human lung cells

“…A549 and BEAS-2B lung cell lines have been used for in vitro assessment of oxidative stress and cytotoxicity following exposure to PM 2.5 and gas-phase pollutants ( Arashiro et al, 2016 ; Jathar et al, 2020 ; Khan et al, 2021 ; Lima de Albuquerque et al, 2021 ; Lin et al, 2017b ; McDonald et al, 2010 ; Zhang et al, 2017 ). Reactive oxygen species (ROS) buildup in exposed cells demonstrates pathophysiological lung conditions ( Qu et al, 2017 ; Zhang et al, 2017 ).…”