The toxic effect of thioacetamide on rat liver in vitro

Staňková, Pavla; Kučera, Otto; Lotková, Halka; Roušar, Tomáš; Endlicher, René; Červinková, Z

doi:10.1016/j.tiv.2010.06.011

Cited by 75 publications

(80 citation statements)

References 53 publications

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“…Thioacetamide is a strong nephrotoxic substrate [2]. By oxidase system function, thioacetamide is metabolized to its toxic metabolites sulfine (sulfoxide) and sulfene (sulfone) which are then spread in several organs, including plasma, liver, kidney, bone marrow, adrenal and other tissues [9,10].…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Omega-3 Against Thioacetamide Induced Lipid and Renal Dysfunction in Male Rats

Moghadamnia

Mokhtari

Khatamsaz

2016

Zahedan J Res Med Sci

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Background: Thioacetamide causes lipid and kidney dysfunction.Omega-3 unsaturated fatty acids prevent the progression of renal diseases. Objectives: This study aimed to assess the protective effects of omega-3 fish oil supplement on thioacetamide induced lipid and kidney dysfunction in male rats. Methods: In this experimental study, 42 male rats were divided into 6 groups of 7: control group sham group which received 0.4 mL olive oil as a solvent, Thioacetamide group receiving thioacetamide at a dose of 150 mg/kg once as intraperitoneal injection, Experimental groups of 1, 2 and 3 which received omega-3 fish oil supplement at the doses of 100, 200, 300 mg/kg orally for 3 months respectively and then they received thioacetamide at the dose of 150 mg/kg intraperitoneally for once. The levels of serum creatinine, BUN, total cholesterol, LDL, HDL, FBS, triglyceride, sodium and potassium were measured. The pathological changes of tissue samples of the kidneys were studied after hematoxylin-eosin staining. The data were analyzed by SPSS-18 software and using one way ANOVA and Tukey as post hoc test. Significant level was considered to be P < 0.05.

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Section: Introductionmentioning

confidence: 99%

The Protective Effect of Omega-3 Against Thioacetamide Induced Lipid and Renal Dysfunction in Male Rats

Moghadamnia

Mokhtari

Khatamsaz

2016

Zahedan J Res Med Sci

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“…One of these industrial toxins is thioacetamide. This toxin induces hepatic centrilobular necrosis, liver cirrhosis and hepatocellular carcinoma (HCC) 18 . According to the results of this study, the values of albumin, bilirubin,LDH, GGT in groups treated with thioacetamide increased significantly compared to the control and sham1 groups.…”

Section: Resultsmentioning

confidence: 99%

Protective Effects of Fish Oil Omega-3 Supplement on Liver-related Biochemical Factors Changes Induced by Thioacetamide in Male Rats

Moghadamnia¹,

Mokhtari²,

Khatamsaz³

2016

Biosci., Biotech. Res. Asia

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Exposure to thioacetamide causes hepatotoxicity and hepatocellular carcinoma in human, while fish oil Omega-3 supplement has anti-inflammatory effects. In this study, the protective effect of Fish oil Omega-3 supplement against liver-related biochemical factors changes induced by thioacetamide in rat is investigated.42 male rats were divided into 6 groups of seven. The control group, The sham 1 group receiving 0.4ml/kg olive oil as the solvent of Fish oil Omega-3 supplement, the sham2 group inter peritonealy receiving a dose of 150 mg/kg thioacetamide at the end of the experiment, the experimental groups 1, 2 and 3 orally receiving a daily dose of 100, 200 and 300 mg/kg Fish oil Omega-3 supplements respectively for 3 month followed by an inter peritoneal dose of 150 mg/kg thioacetamide at the end of the experiment. The serum levels of GGT, LDH, albumin and total protein were measured. Following hematoxylin-eosin staining, liver tissue samples were pathologically studied.The mean level of Albumin showed a significant reduction in experimental groups 1, 2 and 3 receiving thioacetamide. Also, the mean concentration of GGT In the experimental groups 1 and 2 compared to the group receiving thioacetamide decreased significantly; whereas, the mean levels of LDH and total protein showed no significant changes in the experimental groups 1, 2, and 3 (pd"0.05). The results of this study indicate that Fish oil Omega-3 supplement has a protective effect on liver-related biochemical factors changes induced by thioacetamide in rat.

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“…We also demonstrated that hepatic total glutathione (the sum of glutathione (GSH) and oxidized form of glutathione, glutathione disulfide (GSSG)) in HFD mice was significantly lower than that in ND mice after TA dosing (Shirai et al, 2013). GSH is a major biological antioxidant (Lu, 1999;Kretzschmar, 1996;Yuan and Kaplowitz, 2009), and it was reported that TA treatment depleted GSH and increased oxida-tive stress in hepatocytes (Staňková et al, 2010). These results and reports suggested that the amount of hepatic GSH and oxidative stress are likely factors for the mechanism of the attenuated TA-induced hepatic necrosis in HFD mice.…”

Section: Introductionmentioning

confidence: 90%

Hepatic glutathione contributes to attenuation of thioacetamide-induced hepatic necrosis due to suppression of oxidative stress in diet-induced obese mice

et al. 2015

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-We previously reported that hepatic necrosis induced by thioacetamide (TA), a hepatotoxicant, was attenuated in mice fed a high-fat diet (HFD mice) in comparison with mice fed a normal rodent diet (ND mice). In this study, we focused on investigation of the mechanism of the attenuation. Hepatic content of thiobarbituric acid reactive substances (TBARS), an oxidative stress marker, significantly increased in ND mice at 24 and 48 hr after TA administration in comparison to that in vehicle-treated ND mice. At these time points, severe hepatic necrosis was observed in ND mice. Treatment with an established antioxidant, butylated hydroxyanisole, attenuated the TA-induced hepatic necrosis in ND mice. In contrast, in HFD mice, hepatic TBARS content did not increase, and hepatic necrosis was attenuated in comparison with ND mice at 24 and 48 hr after TA dosing. Metabolomics analysis regarding hepatic glutathione, a biological antioxidant, revealed decreased glutathione and changes in the amount of glutathione metabolism-related metabolites, such as increased ophtalmate and decreased cysteine, and this indicated activation of glutathione synthesis and usage in HFD mice. Finally, after treatment with L-buthionine-S,R-sulfoxinine, an inhibitor of glutathione synthesis, TA-induced hepatic necrosis was enhanced and hepatic TBARS contents increased after TA dosing in HFD mice. These results suggested that activated synthesis and usage of hepatic GSH, which suppresses hepatic oxidative stress, is one of the factors that attenuate TA-induced hepatic necrosis in HFD mice.

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The toxic effect of thioacetamide on rat liver in vitro

Cited by 75 publications

References 53 publications

The Protective Effect of Omega-3 Against Thioacetamide Induced Lipid and Renal Dysfunction in Male Rats

The Protective Effect of Omega-3 Against Thioacetamide Induced Lipid and Renal Dysfunction in Male Rats

Protective Effects of Fish Oil Omega-3 Supplement on Liver-related Biochemical Factors Changes Induced by Thioacetamide in Male Rats

Hepatic glutathione contributes to attenuation of thioacetamide-induced hepatic necrosis due to suppression of oxidative stress in diet-induced obese mice

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