The Toxic Effect Of Sildenafil Citrate On Adult Albino Rat Testis And The Possible Protective Role Of Royal Jelly (Histological And Immunohistochemical Study)

Abstract

Background: Phosphodiesterase inhibitor was appearing as a magic tool in treatment of erectile dysfunction, however, it might lead to harmful effects in high dose and long term administration. The aim of the work: This study aimed to evaluate the toxic effect of sildenafil citrate on the histological structure of the testis of adult albino ratsand the possible protective role of royal jelly. Materials and methods: Thirty five adult male albino rats were divided into 3 groups; the control group divided into (subgroup Ca, Cb and Cc), experimental group divided into (subgroup Ea and Eb) and treated group divided into (subgroup Ta and Tb). In the experimental group sildenafil citrate was given at a dose of 9 mg/kg in subgroup Ea and 13.5mg/kg in subgroup Eb orally daily for 30 days. In the treated group rats were treated by royal jelly at a dose of 100 mg/kg for 30 days orally after stoppage of sildenafil citrate. In the present study, the sperm count, histological and immunohistochemical studies were evaluated. Result : Daily oral administration of sildenafil citrate (9mg/kg and13.5 mg/kg) for 30 day had reduced the sperm count and induced apparent degeneration with detached, distorted germ cell and congested blood vessels with significant decrease in Proliferating cell nuclear antigen Labeling Index (PCNA-LI). After royal jelly administration, there was an improvement in sperm count and histopathological changes induced by sildenafil citrate with a significant increase in PCNA-LI. Conclusion: Long-term daily use of Sildenafil citrate produced histological changes in the testis and reduced the spermatogenesis with a significant decrease in sperm count and these side effects can be prevented by using royal jelly.

Effects of Cultured Cordycep militaris on Sexual Performance and Erectile Function in Streptozotocin-Induced Diabetic Male Rats

“…In contrast, the number of sperm and its motility were relatively unchanged in diabetic rats treated with sildenafil citrate. This drug seemed to consistently demonstrate an unusual side effect on the histological structure of the testis of adult albino rats when given for a long time [ 29 ].…”

EFFECTS OF SILDENAFIL ON THE TESTES OF THE NONDIABETIC AND DIABETIC ADULT ALBINO RATS (Light and Electron Microscopic Study)

“…Seventy-two hours later, blood glucose level was determined to confirm induction of diabetes (normal blood glucose level of the rats is 50-95 mg /dl fasting and 70-130 post prandial). Rats with blood glucose level >250 mg/dl were considered diabetic and included in the experiment Then, Group III-A: received IPI of SC 9 mg/kg/d for 2 month 7 .…”

Effect of Nicotine on the Rat’s Tongue and the Possible Protective Role of Royal Jelly Light and Scanning Electron Microscopic Study

“…Group III (Nicotine+Royaljellygroup): included 10 adult male rats subjected to the same dose of nicotine +Royal jelly(The capsules were dissolved in corn oil for oral administration). Rats were administered 100 mg/kg royal jelly (11) for 28 days.…”