The topography of cholinergic transmission in the mechanism of drug action at muscarinic synapses of the guinea-pig ileum

Kadlec, O.; Mašek, K.; Šeferna, I.

doi:10.1016/0014-2999(87)90709-6

Cited by 5 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The postsynaptic site of action and the difference in antagonistic potencies of pirenzepine and atropine corresponded well to their action at M2-muscarinic receptors of the smooth muscle (North et al 1985;Freedman et al 1988;Jeck et al 1988;Kilbinger & Stein 1988). The inhibitory effects of both drugs did not differ appreciably when applied either to the oral or abordl segments suggesting that their main effect was postsynaptic as it did not indicate a different presynaptic arrangement in the respective segments (Kadlec et al 1987(Kadlec et al , 1990b.…”

Section: Synaptic Effectsmentioning

confidence: 56%

“…The effects of cholinomimetic drugs on postsynaptic Mzreceptors causing contractions or on presynaptic M2-receptors causing autoinhibition of transmitter release have been extensively studied (Kilbinger 1984). The main aim of our study, however, was to distinguish the non-synaptic effects of cholinomimetic drugs which might be explained by their interference with propagation of action potentials in cholinergic nerve terminals; such study was enabled by using the triple bath arrangement (Kadlec et al 1985(Kadlec et al , 1987.…”

Section: Discussionmentioning

confidence: 99%

“…All the responses were neurogenic as the addition of tetrodotoxin ( I jcmol L I) to the oral, middle or aboral compartments separately always abolished twitches of the aboral segment; furthermore, these responses were not affected by the addition of hexamethonium, ( + )-tubocurarine or papaverine to the middle segment (Kadlec et al 1985(Kadlec et al , 1987.…”

Section: Methodsmentioning

confidence: 99%

“…Contractions were also evoked by bolus additions of acetylcholine (1.1 pmol in 10 p L ) added to the solution superfusing the aboral segment immediately before reaching it. Contractions of one peripheral segment caused no mechanical artifacts in the contralateral segment (Kadlec et al 1985(Kadlec et al . 1987).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work a method of neurogenic stimulation of myenteric plexus-longitudinal muscle strip from the guinea-pig ileum in a triple bath was described (Kadlec et al 1985(Kadlec et al , 1987. The strip was pulled through two rubber membranes dividing it into three segments supplied separately by perfusing solutions.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Non-synaptic Cholinergic Modulation of Neurogenic Twitches of the Guinea-pig Ileum

Kadlec

Hynie

Mašek

et al. 1991

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

The effect of cholinergic and anticholinergic compounds on conduction of neuronal excitation has been studied in myenteric plexus-longitudinal muscle strips from the guinea-pig ileum. A preparation in a special triple bath was drawn through two rubber membranes dividing the strip into three segments. Neurogenic stimulation of the oral segment set up nerve action potentials propagating aborally across the middle segment (10 mm) so that the aboral segment might be also invaded, eventually. Drugs were added to the middle segment to affect neuronal propagation (non-synaptic effects) which was monitored by twitch height of the aboral segment. The application of acetylcholine to the middle segment augmented aboral twitches. The effects of nicotine, pilocarpine and oxotremorine were selectively blocked by (+)-tubocurarine, pirenzepine and atropine, respectively. The effect of acetylcholine was suppressed by pirenzepine and atropine and mimicked by doubling of KCl concentration. The effect of acetylcholine may be thus explained by the facilitated propagation of nerve action potentials in partially depolarized cholinergic terminals via stimulation of muscarinic receptors. The adenylate cyclase system is not directly involved in the mechanism of muscarinic facilitation of neuronal propagation in the terminals; however, it may participate in the modulation of a final common effector mechanism.

show abstract

Section: Synaptic Effectsmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Non-synaptic Cholinergic Modulation of Neurogenic Twitches of the Guinea-pig Ileum

Kadlec

Hynie

Mašek

et al. 1991

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

The effect of substance P on nerve action potential propagation and cholinergic transmission in the myenteric plexus of the guinea-pig ileum

Ševčı́k¹,

Kadlec²,

Šeferna

1990

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

The effect of substance P on nerve terminals in myenteric plexus of the guinea-pig ileum was investigated. Neurogenic twitches of the myenteric plexus longitudinal muscle strip were recorded. Twitches of the strip portion where excitation involved the most distal parts of cholinergic nerve terminals were more increased by local application of substance P (0.1 and 0.4 nmol/l) than twitches of the portion where excitation involved both distal and proximal parts of nerve terminals. Substance P addition to a portion of the strip conducting nerve action potentials to invade the neighbouring strip portion also augmented twitches of the latter portion so that the interference with the propagation process was considered. The effect of substance P was poorly antagonized by the addition of a substance P antagonist, (D-Arg1, D-Pro2, D-Trp7,9, Leu11)-substance P. Compound nerve action potentials were evoked in strands of fibres of the myenteric plexus by low-frequency train stimulation (1 Hz). The addition of substance P prevented a decrease of the amplitude of responses observed under control conditions. Using high-frequency train stimulation (30 Hz) the amplitude of responses to impulses 2-7 was augmented over that to the first impulse; substance P further increased such facilitation regularly. It seems that substance P might promote nerve action potential invasion of the distal parts of nerve terminals.

show abstract

An attempt to localize the site of action of different agents within cholinergic motor neurones of the myenteric plexus-longitudinal muscle preparation of the guinea pig ileum by the triple bath method

Kadlec¹,

Šeferna

Ševčı́k³

1991

Neuropharmacology

View full text Add to dashboard Cite

The topography of cholinergic transmission in the mechanism of drug action at muscarinic synapses of the guinea-pig ileum

Cited by 5 publications

References 24 publications

Non-synaptic Cholinergic Modulation of Neurogenic Twitches of the Guinea-pig Ileum

Non-synaptic Cholinergic Modulation of Neurogenic Twitches of the Guinea-pig Ileum

The effect of substance P on nerve action potential propagation and cholinergic transmission in the myenteric plexus of the guinea-pig ileum

An attempt to localize the site of action of different agents within cholinergic motor neurones of the myenteric plexus-longitudinal muscle preparation of the guinea pig ileum by the triple bath method

Contact Info

Product

Resources

About