The tomato polygalacturonase gene and ripening-specific expression in transgenic plants

Bird, Colin R.; Smith, Christopher J.; Ray, John A.; Moureau, P.; Bevan, Michael W.; Bird, Alison; Hughes, Stephen; Morris, P. I.; Grierson, Don; Schuch, Wolfgang

doi:10.1007/bf00017465

Cited by 227 publications

(146 citation statements)

References 41 publications

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“…The results are similar to the findings of scientists who worked on the role of PG during fruit development in various kinds of fruits and found a correlation, as PG activity and softening occurs simultaneously in cherry (Barrett and Gonzalez, 1994), date (Hasegawa et al, 1969), durian (Ketsa and Daengkanit, 1999), kiwifruit (Wang et al, 2000), mango (Ketsa et al, 1998), papaya (Paull and Chen, 1983), pear (Ahmed and Labavitch, 1980), pepper (Rao and Paran, 2003;Arancibia and Motsenbocker, 2006), tobacco (Osteryoung et al, 1990), and tomato (Hobson, 1965;Crookes and Grierson, 1983;Huber, 1983;DellaPenna et al, 1987;Bird et al, 1988;Biggs and Handa, 1989;Smith et al, 1990;Fenwick et al, 1996).…”

Section: Discussionsupporting

confidence: 88%

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Ahmed

Gong²,

Khan³

et al. 2011

Genet. Mol. Res.

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Section: Discussionsupporting

confidence: 88%

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Ahmed

Gong²,

Khan³

et al. 2011

Genet. Mol. Res.

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“…tumefaciensmediated transformation of tomato stem explants was carried out as described (15), using kanamycin resistance as a selection marker.…”

Section: Transformation Of Tomato Plants and A Tumefaciensmentioning

confidence: 99%

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

Fraser

Römer

Shipton

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

437

278

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Phytoene synthase from the bacterium Erwinia uredovora (crtB) has been overexpressed in tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T 0) were 2-4-fold higher than the controls, whereas phytoene, lycopene, ␤-carotene, and lutein levels were increased 2.4-, 1.8-, and 2.2-fold, respectively. The biosynthetically related isoprenoids, tocopherols plastoquinone and ubiquinone, were unaffected by changes in carotenoid levels. The progeny (T 1 and T2 generations) inherited both the transgene and phenotype. Determination of enzyme activity and Western blot analysis revealed that the CRTB protein was plastid-located and catalytically active, with 5-10-fold elevations in total phytoene synthase activity. Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway. The activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diphosphate synthase, and incorporation of isopentenyl diphosphate into phytoene) were not significantly altered by the presence of the bacterial phytoene synthase.carotenoids ͉ metabolic engineering

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“…Polygalacturonases (PGs) are produced by various organisms, such as plants (Bird et al 1988;Hadfield and Benett 1998), bacteria (Jayani et al 2010;Tariq and Latif 2012;Chen et al 2014) and fungi (Martins et al 2013;Ortega et al 2014;Cheng et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice

Anand

Yadav

2017

3 Biotech

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Polygalacturonases (PG) represent an important member of pectinases group of enzymes with immense industrial applications. A fungal strain Aspergillus niger MTCC478 was used for the production of polygalacturonase both under submerged and solid-state fermentation condition. Further its production was optimized under solid-state fermentation condition with media comprising of wheat bran and tea extract. Purification of an exo-PG was achieved by acetone precipitation (60-90%) and CMcellulose column chromatography revealing 15.28-fold purification with a specific activity of 33.47 U/mg protein and 1.2% yield. A relative molecular mass of purified PG was approximately 124.0 kDa. The pH and temperature optimum was found to be 4 and 50°C, respectively. The k cat and K m value for degradation of PGA by the purified enzyme was found to be 194 s -1 and 2.3 mg/mL, respectively. Cu 2? was found to enhance the PG activity while Ag ? completely inhibited the enzyme activity. The application of the purified PG in orange juice clarification was elucidated.

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The tomato polygalacturonase gene and ripening-specific expression in transgenic plants

Cited by 227 publications

References 41 publications

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice

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