The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase

Gonorazky, Gabriela; Ramírez, Leonor; Abd-El-Haliem, Ahmed; Vossen, Jack H.; Lamattina, Lorenzo; Have, Arjen ten; Joosten, M.H.A.J.; Laxalt, Ana M.

doi:10.1016/j.jplph.2014.02.008

Cited by 25 publications

(24 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned previously, it was demonstrated that SlPLC2 is involved in xylanase-induced defense gene expression (Gonorazky et al, 2014). To investigate whether SlPLC2 plays a role in tomato-B.…”

Section: Resultsmentioning

confidence: 88%

“…As mentioned previously, it was demonstrated that SlPLC2 is involved in xylanase‐induced defense gene expression (Gonorazky et al ., ). To investigate whether SlPLC2 plays a role in tomato‐ B. cinerea interaction, SlPLC2 expression was knocked down by VIGS employing a tobacco‐rattle virus (TRV) construct (Gonorazky et al ., ). As a negative control we used a TRV with the β‐glucuronidase ( GUS ) gene (TRV: GUS ), which has no homologues in plants.…”

Section: Resultsmentioning

confidence: 97%

“…As a negative control we used a TRV with the β‐glucuronidase ( GUS ) gene (TRV: GUS ), which has no homologues in plants. Under normal growth conditions, the TRV: SlPLC2 plants displayed no apparent morphological alterations, as reported earlier (Gonorazky et al ., ). Silencing specificity of SlPLC2 was studied by measuring transcript levels of the six SlPLCs in TRV: GUS and TRV: SlPLC2 leaflets inoculated with B. cinerea .…”

Section: Resultsmentioning

confidence: 97%

“…Particularly in tomato, xylanase treatment increases SlPLC2 and SlPLC5 transcript levels (Gonorazky et al ., ). From these two SlPLC genes, SlPLC2 showed the highest induction (Gonorazky et al ., ). Here we demonstrate that SlPLC2 expression was induced in tomato plants inoculated with B. cinerea , together with SlPLC3, SlPLC4 and SlPLC5 .…”

Section: Discussionmentioning

confidence: 97%

“…We have recently demonstrated that xylanase induces an increase of SlPLC2 and SlPLC5 transcript levels both in tomato cell suspensions and tomato plants (Gonorazky et al, 2014). We found by VIGS assays that SlPLC2, but not SlPLC5, is required for xylanase-induced expression of the SA-defense gene marker Pathogenesis Related1 (SlPR1) and the HR tomato gene marker Hypersensitive Response 203J (SlHSR203J) (Gonorazky et al, 2014). Since xylanase is a PAMP, the aim of this work was to investigate whether SlPLC2 plays a role in plant-pathogen interactions.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

Gonorazky

Guzzo²,

Abd-El-Haliem

et al. 2016

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase-induced expression of defense-related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked-down the expression of SlPLC2 by virus-induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)-defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non-silenced infected plants, while transcripts of the jasmonic acid (JA)-defense gene markers Proteinase Inhibitor I and II (SlPI-I and SlPI-II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.

show abstract

Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

Gonorazky

Guzzo²,

Abd-El-Haliem

et al. 2016

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

Nicotiana benthamiana, A Popular Model for Genome Evolution and Plant–Pathogen Interactions

Pombo

Rosli

Fernandez-Pozo

et al. 2020

Compendium of Plant Genomes

View full text Add to dashboard Cite

Long-Distance Lipid Signaling and its Role in Plant Development and Stress Response

Barbaglia¹,

Hoffmann-Benning²

2016

Subcellular Biochemistry

View full text Add to dashboard Cite

Lipids are important signaling compounds in plants. They can range from small lipophilic molecules like the dicarboxylic acid Azelaic acid to complex phosphoglycerolipids and regulate plant development as well as the response to biotic and abiotic stress. While their intracellular function is well described, several lipophilic signals are known to be found in the plant phloem and can, thus, also play a role in long-distance signaling. Mostly, they play a role in the pathogen response and systemic acquired resistance. This is particularly true for oxylipins, dehydroabietinal, and azelaic acid. However, several phospholipids have now been described in phloem exudates. Their intracellular function as well as implications and a model for long-distance signaling are discussed in this chapter.

show abstract

The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase

Cited by 25 publications

References 25 publications

Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

Nicotiana benthamiana, A Popular Model for Genome Evolution and Plant–Pathogen Interactions

Long-Distance Lipid Signaling and its Role in Plant Development and Stress Response

Contact Info

Product

Resources

About