The Tomato MIXTA-like Transcription Factor Coordinates Fruit Epidermis Conical Cell Development and Cuticular Lipid Biosynthesis and Assembly

Lashbrooke, Justin Graham; Adato, Avital; Lotan, O.; Alkan, Noam; Tsimbalist, Tatiana; Rechav, Katya; Forment, Javier; Widemann, Émilie; Grausem, Bernard; Pinot, Franck; Granell, Antonio; Costa, Fabrizio; Aharoni, Asaph

doi:10.1104/pp.15.01145

Cited by 63 publications

(90 citation statements)

References 99 publications

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“…The differentially expressed TFs included those with a possible link to the circadian clock, with two genes (Solyc01g095030 and Solyc10g084370) encoding MYB-related TFs of the REVEILLE family (Farinas and Mas, 2011), one of which was expressed at lower levels in gpat6-a, and six genes encoding CONSTANS-like TFs (Valverde, 2011), one of which (Solyc12g005660) was expressed at much lower levels. Despite the large number of differentially expressed TFs identified, the group did not include TFs that have been reported to regulate cuticle formation in tomato or other species (Shi et al, 2013;Borisjuk et al, 2014;Hen-Avivi et al, 2014), other than a MIXTA-like MYB TF (Oshima and Mitsuda, 2013), whose role in tomato was described recently (Lashbrooke et al, 2015). We observed that the expression of SlMIXTA-like (Solyc02g088190) was lower in the gpat6-a mutant than in the wild type (Supplemental Table S5).…”

Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness and mentioning

confidence: 60%

“…We observed that the expression of SlMIXTA-like (Solyc02g088190) was lower in the gpat6-a mutant than in the wild type (Supplemental Table S5). Moreover, 10 cutin-and wax-related genes that were differentially expressed between the wild type and the gpat6-a mutant (Table IV) were identified previously as SlMIXTA-like targets (Lashbrooke et al, 2015).…”

Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness and mentioning

confidence: 99%

“…Since then, many TFs belonging to several families (AP2/ ERF, DREB/CBF, MYB, HD-Zip IV, and WW domain proteins) have been identified in various plant species, including those that regulate cuticle formation in fleshy fruits (Borisjuk et al, 2014;Hen-Avivi et al, 2014). Further studies have revealed the identity of target genes of these TFs, most of which are genes involved in fatty acid, phenylpropanoid, and flavonoid metabolism (Adato et al, 2009;Lashbrooke et al, 2015).…”

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confidence: 99%

“…Collectively, the wax fraction is typically a complex mixture composed of derivatives of very-long-chain fatty acids, mainly alkanes and alcohols, but it may also include various secondary metabolites, such as the triterpenol compounds amyrins, flavonoids, and sterols (Bernard and Joubès, 2013). The synthesis of cuticle is tightly coordinated with developmental processes, such as the formation of epidermal cells during plant and fruit growth (Javelle et al, 2011;Lashbrooke et al, 2015). Environmental cues also may affect cuticle formation, as is the case with drought stress triggering changes in the cuticle via abscisic acid signaling .…”

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The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

et al. 2016

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The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6). To this end, a backcross population (BC 1 F 2 ) segregating for the glossy trait was phenotyped. Individuals displaying either a wild-type or a glossy fruit trait were then pooled into bulked populations and submitted to whole-genome sequencing prior to mutation frequency analysis. This revealed that the causal point mutation in the gpat6-a mutant introduces a charged amino acid adjacent to the active site of a GPAT6 enzyme. We further showed that this mutation completely abolished the GPAT activity of the recombinant protein. The gpat6-a mutant showed perturbed pollen formation but, unlike a gpat6 mutant of Arabidopsis (Arabidopsis thaliana), was not male sterile. The most striking phenotype was observed in the mutant fruit, where cuticle thickness, composition, and properties were altered. RNA sequencing analysis highlighted the main processes and pathways that were affected by the mutation at the transcriptional level, which included those associated with lipid, secondary metabolite, and cell wall biosynthesis.

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Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness and mentioning

confidence: 60%

Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness and mentioning

confidence: 99%

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confidence: 99%

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The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

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“…To date, no genes responsible for the extracellular polymerization of the suberin polymer or for the synthesis of the aromatic monomers have been described. While several transcription factors influencing plant cutin and wax formation have been identified Oshima et al, 2013;Shi et al, 2011Shi et al, , 2013Lashbrooke et al, 2015aLashbrooke et al, , 2015b, only a single regulatory gene of suberin synthesis has been characterized. The recently described MYB factor AtMYB41 was demonstrated to be a positive regulator of suberin biosynthesis in Arabidopsis thaliana under stress conditions.…”

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confidence: 99%

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

et al. 2016

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Suberin, a polymer composed of both aliphatic and aromatic domains, is deposited as a rough matrix upon plant surface damage and during normal growth in the root endodermis, bark, specialized organs (e.g., potato [Solanum tuberosum] tubers), and seed coats. To identify genes associated with the developmental control of suberin deposition, we investigated the chemical composition and transcriptomes of suberized tomato (Solanum lycopersicum) and russet apple (Malus x domestica) fruit surfaces. Consequently, a gene expression signature for suberin polymer assembly was revealed that is highly conserved in angiosperms. Seed permeability assays of knockout mutants corresponding to signature genes revealed regulatory proteins (i.e., AtMYB9 and AtMYB107) required for suberin assembly in the Arabidopsis thaliana seed coat. Seeds of myb107 and myb9 Arabidopsis mutants displayed a significant reduction in suberin monomers and altered levels of other seed coat-associated metabolites. They also exhibited increased permeability, and lower germination capacities under osmotic and salt stress. AtMYB9 and AtMYB107 appear to synchronize the transcriptional induction of aliphatic and aromatic monomer biosynthesis and transport and suberin polymerization in the seed outer integument layer. Collectively, our findings establish a regulatory system controlling developmentally deposited suberin, which likely differs from the one of stressinduced polymer assembly recognized to date.

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MYB Genes Involved in Domestication and Crop Improvement

Albert

Allan

2021

Annual Plant Reviews Online

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Domestication of the world's crop species has been achieved through improvements in yield, and product qualities, with or without the guidance of scientific knowledge. These selection pressures are applied by breeders (and before them, farmers) both intentionally and unintentionally to improve crop features, which have often been grouped into ‘production traits’ and ‘quality traits’. New approaches to breeding that incorporate genome sequencing, mapping, and identification of genes underlying traits have revealed new variants in the family of transcription factors (TFs) called MYBs as driving important features in both these categories of domestication traits. In this article, we review the progress in understanding the role of MYB TFs in crop performance, as well as reasons to predict further gains in yield and quality centred on the MYB TF family.

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The Tomato MIXTA-like Transcription Factor Coordinates Fruit Epidermis Conical Cell Development and Cuticular Lipid Biosynthesis and Assembly

Cited by 63 publications

References 99 publications

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

MYB Genes Involved in Domestication and Crop Improvement

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