The tolerance of theArabidopsisdefense hormone receptor mutantcoi1against the vascular pathogenVerticillium longisporumis not due to increased levels of the active hormone jasmonoyl-isoleucine

Ralhan, Anjali; Thurow, Corinna; Gatz, Christiane

doi:10.4161/psb.27008

Cited by 3 publications

(4 citation statements)

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“…We have shown previously that – similar to coi1‐1 sid2‐2 – the JA‐Ile‐deficient coi1‐t aos double mutant is as tolerant as coi1‐t (Ralhan et al ., 2013). Likewise, PGM and PRLIP2 expression was as high in coi1‐t aos as in coi1‐t , demonstrating that JA‐Ile does not induce the expression of these genes in the absence of its receptor (Figure S6).…”

Section: Resultsmentioning

confidence: 99%

“…Since growth of V. longisporum or F. oxysporum is not inhibited in the root (Ralhan et al, 2013;Thatcher et al, 2009) and since the pathway is not constitutively activated in coi1 shoots (Kloek et al, 2001), a contribution to tolerance can only be assumed under the premises that extracellular defense compounds travel from the root to the shoot, where they might accumulate to interfere with fungal growth. It remains to be elucidated whether the tolerance phenotype can be reverted to susceptibility by suitable mutations of the above-mentioned regulators in the coi1 background.…”

Section: Discussionmentioning

confidence: 99%

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The jasmonoyl‐isoleucine receptor CORONATINE INSENSITIVE1 suppresses defense gene expression in Arabidopsis roots independently of its ligand

et al. 2021

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Summary The F‐box protein CORONANTINE INSENSITIVE1 (COI1) serves as the receptor for the plant hormone jasmonoyl‐isoleucine (JA‐Ile). COI1, its co‐receptors of the JASMONATE ZIM‐domain (JAZ) protein family, and JA‐Ile form a functional unit that regulates growth or defense mechanisms in response to various stress cues. Strikingly, COI1, but not JA‐Ile, is required for susceptibility of Arabidopsis thaliana towards the soil‐borne vascular pathogen Verticillium longisporum. In order to obtain marker genes for further analysis of this JA‐Ile‐independent COI1 function, transcriptome analysis of roots of coi1 and allene oxide synthase (aos) plants (impaired in JA biosynthesis) was performed. Intriguingly, nearly all of the genes that are differentially expressed in coi1 versus aos and wild type are constitutively more highly expressed in coi1. To support our notion that COI1 acts independently of its known downstream signaling components, coi1 plants were complemented with a COI1 variant (COI1AA) that is compromised in its interaction with JAZs. As expected, these plants showed only weak induction of the expression of the JA‐Ile marker gene VEGETATIVE STORAGE PROTEIN2 after wounding and remained sterile. On the other hand, genes affected by COI1 but not by JA‐Ile were still strongly repressed by COI1AA. We suggest that COI1 has a potential moonlighting function that serves to repress gene expression in a JA‐Ile‐ and JAZ‐independent manner.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The jasmonoyl‐isoleucine receptor CORONATINE INSENSITIVE1 suppresses defense gene expression in Arabidopsis roots independently of its ligand

et al. 2021

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“…We have shown previously thatsimilar to coi1-1 sid2-2 -the JA-Ile-deficient coi1-t aos double mutant is as tolerant as coi1-t (Ralhan et al, 2013). Likewise, PGM and PRLIP2 expression was as high in coi1-t aos as in coi1-t, demonstrating that JA-Ile does not induce the expression of these genes in the absence of its receptor (Figure S6).…”

Section: Coi1 Suppresses Gene Expression In the Absence Of Jasmonoyl-...mentioning

confidence: 73%

“…Since growth of V. longisporum or F. oxysporum is not inhibited in the root (Ralhan et al, 2013;Thatcher et al, 2009) and since the pathway is not constitutively activated in coi1 shoots , a contribution to tolerance can only be assumed under the premises that extracellular defense compounds travel from the root to the shoot, where they might accumulate to interfere with fungal growth. It remains to be elucidated whether the tolerance phenotype can be reverted to susceptibility by suitable mutations of the above-mentioned regulators in the coi1 background.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the JA-Ile-independent function of COI1 in Arabidopsis thaliana upon infection with Verticillium longisporum

Ulrich¹

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CORONATINE INSENSITIVE 1 (COI1) perceives the plant hormone jasmonoyl-isoleucine (JA-Ile) together with proteins of the JASMONATE ZIM-domain (JAZ) family. JA-Ile induces signalling cascades in defence and developmental processes. It has been shown that in Arabidopsis thaliana, COI1 without its ligand conveys susceptibility to the soil-born vascular pathogen Verticillium longisporum. Grafting experiments have shown that presence of COI1 in roots mediates susceptibility to the pathogen. Root transcriptome analysis has revealed that a number of salicylic acid defence-associated genes are constitutively expressed in coi1. The observation that COI1 acts as a JA-Ile-independent repressor of root gene expression led us to postulate that this novel COI1 function operates independently of the canonical JA signalling machinery.In this thesis, we show that coi1 plants complemented with a COI1 protein, that was severely impaired in its interaction with JAZ proteins (COI1 AA ), were compromised in wound-induced induction of the JA-signalling marker gene VEGETATIVE STORAGE PROTEIN 2 (VSP2).Moreover, COI1 AA could not restore fertility in sterile coi1 plants. In contrast, COI1 AA was able to repress gene expression in roots. Hence, in roots, COI1 has a second function other than its role in JA-Ile perception, in which it acts as a suppressor of defence gene expression independently of JA-Ile and most likely independently of JAZ proteins. We furthermore show that after infection with V. longisporum, approximately half of the COI1-repressed genes in roots are induced to similar levels as in coi1. We hence postulate that COI1-mediated repression is inactivated upon infection with V. longisporum leading to induction of these genes. Gene induction requires the transcription factor SYSTEMIC ACQUIRED RESISTANCE 1 (SARD1) which is itself repressed by COI1. Equally, constitutive expression of genes in coi1 was abolished by mutations in SARD1 and its close homologue CALMODULIN BINDING PROTEIN 60-LIKE G. In contrast, overexpression of SARD1 in wild-type roots did not lead to activation of gene expression, likely because the repressive effect of COI1 on gene expression could not be overcome. The repressor function of COI1 was only observed in roots and not in shoots. As roots need to balance perception of microbe-associated molecular patterns with maintaining an intact rhizosphere, we speculate that COI1 acts as a regulator of the onset of defence responses in roots.

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An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis

et al. 2016

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An ethylene response-related factor, GbERF1-like, from Gossypium barbadense cv. '7124' involved in the defence response to Verticillium dahliae was characterized. GbERF1-like transcripts present ubiquitously in various tissues, with higher accumulation in flower organs. GbERF1-like was also responsive to defence-related phytohormones and V. dahliae infection. The downregulation of GbERF1-like increased the susceptibility of cotton plants to V. dahliae infection, while overexpression of this gene improved disease resistance in both cotton and Arabidopsis, coupled with activation of the pathogenesis-related proteins. Further analysis revealed that genes involved in lignin synthesis, such as PAL, C4H, C3H, HCT, CCoAOMT, CCR and F5H, showed higher expression levels in the overexpressing cotton and Arabidopsis lines and lower expression levels in the RNAi cotton lines. The expression levels of these genes increased obviously when the GbERF1-like-overexpressing plants were inoculated with V. dahliae. Meanwhile, significant differences in the content of whole lignin could be found in the stems of transgenic and wild-type plants after inoculation with V. dahliae, as revealed by metabolic and histochemical analysis. More lignin could be detected in GbERF1-like-overexpressing cotton and Arabidopsis but less in GbERF1-like-silencing cotton compared with wild-type plants. The ratio of S and G monomers in GbERF1-like-overexpressing cotton and Arabidopsis increased significantly after infection by V. dahliae. Moreover, our results showed that the promoters of GhHCT1 and AtPAL3 could be transactivated by GbERF1-like in vivo based on yeast one-hybrid assays and dual-luciferase reporter assays. Knockdown of GhHCT1 in GbERF1-like over-expressing cotton decreases resistance to V. dahliae. Collectively, our results suggest that GbERF1-like acts as a positive regulator in lignin synthesis and contributes substantially to resistance to V. dahliae in plants.

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The tolerance of theArabidopsisdefense hormone receptor mutantcoi1against the vascular pathogenVerticillium longisporumis not due to increased levels of the active hormone jasmonoyl-isoleucine

Cited by 3 publications

References 5 publications

The jasmonoyl‐isoleucine receptor CORONATINE INSENSITIVE1 suppresses defense gene expression in Arabidopsis roots independently of its ligand

The jasmonoyl‐isoleucine receptor CORONATINE INSENSITIVE1 suppresses defense gene expression in Arabidopsis roots independently of its ligand

Analysis of the JA-Ile-independent function of COI1 in Arabidopsis thaliana upon infection with Verticillium longisporum

An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis

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