The TOL (pWW0) catabolic plasmid

Burlage, Robert S.; Hooper, S W; Sayler, Gary S.

doi:10.1128/aem.55.6.1323-1328.1989

Cited by 116 publications

(33 citation statements)

References 53 publications

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“…The potential of this approach was first demonstrated by Reineke and Knackmuss in 1980 [121]. They noted that as a consequence of obtaining the TOL plasmid (reviewed in [122]), Pseudornonas sp. B13 obtained the ability to grow on 4-chloro-and 3,5-dichlorobenzoate.…”

Section: Prospectsmentioning

confidence: 99%

Ecological fate, effects and prospects for the elimination of environmental polychlorinated biphenyls (PCBs)

Hooper¹,

Pettigrew

Sayler

1990

Enviro Toxic and Chemistry

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Polychloiinated biphenyls (PCBs) present an environmental health hazard of global scale and man-made origin. Their impact on nearly every member of the biota results in a wide range of interacting negative effects. Due to their chemical nature, these recalcitrant toxicants are poised to enter and bioaccumulate in the food web. Recent advances in biodegradation research and molecular biology have shown that natural microorganisms and genetically modified bacterial strains could be used to decontaminate PCB-containing sites. The aim of this review is to summarize the chemistry, emironmental fate and toxicological effects of PCBs and to evaluate the currently available technologies for nondisruptive elimination of PCBs from the environment.

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Section: Prospectsmentioning

confidence: 99%

Ecological fate, effects and prospects for the elimination of environmental polychlorinated biphenyls (PCBs)

Hooper¹,

Pettigrew

Sayler

1990

Enviro Toxic and Chemistry

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“…The genes encoding PCMH and p ‐hydroxybenzaldehyde dehydrogenase are organized as an operon ( pchACXF in P. putida NCIMB 9866 and pcuCAXB in P. mendocina KR1), with pchA / pcuC encoding the second and pchCF / pcuAB encoding the first enzyme of the p ‐cresol metabolic pathway (Burlage et al , 1989; Wright & Olsen, 1994). The gene designated as pchX / pcuX encodes a protein of unknown function (Wright & Olsen, 1994; Cronin et al , 1999).…”

Section: Introductionmentioning

confidence: 99%

Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-cresol-degradative strains of Pseudomonas fluorescens

Jõesaar

Heinaru

Viggor

et al. 2010

FEMS Microbiology Ecology

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p-Cresol methylhydroxylase (PCMH), a key enzyme responsible for the catabolism of p-cresol via the protocatechuate ortho pathway, was used as a tool to characterize catabolic differences between phenol- and p-cresol-degrading Pseudomonas fluore-scens strains PC18 and PC24. Although both strains catabolize p-cresol using PCMH, different whole-cell kinetic parameters for this compound were revealed. Affinity for the substrate and the specific growth rate were higher in PC18, whereas maximum p-cresol tolerance was higher in PC24. In addition, PCMH of strain PC18 was induced during growth on phenol. In both strains, the pchACXF operon, which encodes p-hydroxybenzaldehyde dehydrogenase and PCMH, was sequenced. Transcriptional regulation of these operons by PchR, a putative sigma(54)-dependent regulator, was shown. Although the promoters of these operons resembled sigma(54)-controlled promoters, they differed from the consensus sequence by having T instead of C at position -12. Complementation assays confirmed that the amino acid sequence differences of the PchR regulators between the two strains studied led to different effector-binding capabilities of these proteins: (1) phenol was a more efficient effector for PchR of PC18 than p-cresol, (2) phenol did not activate the regulator of PC24, and (3) both regulators responded similarly to p-cresol.

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“…The upper pathway (xylCAB) encodes the modification of toluene and xylenes to benzoate and toluates. The lower pathway (xylDLEGFJKIH) encodes the degradation of benzoate and toluates to acetaldehyde and pyruvate (14–17). In the TOL operon the binding of toluene and related compounds to the transcriptional activator (xylR) results in an activating interaction between xylR and the promoter sequence (termed Pu).…”

Section: Introductionmentioning

confidence: 99%

“…So, this operon can be used to design biosensor cells by fusing the reporter gene to the upstream sequence of the Pu promoter (18,19). In the TOL operon, a second transcriptional activator, xylS, binds benzoate and activates transcription of enzymes responsible for breakdown of benzoate to Krebs cycle intermediates (16,20,21). So, the presence of xylS as well as xylR in this bacterial whole‐cell biosensor enables us to detect metabolites such as benzoate.…”

Section: Introductionmentioning

confidence: 99%

Design and Characterization of an Aequorin‐based Bacterial Biosensor for Detection of Toluene and Related Compounds

Zeinoddini

Khajeh²,

Behzadian

et al. 2010

Photochem & Photobiology

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An aequorin-based Escherichia coli strain JM109 biosensor was constructed and characterized for its potential to detect toluene and related compounds in aqueous solutions. The biosensor was constructed based on a PGL2 plasmid carrying the lower pathway promoter (Pu) of the xyl operon of Pseudomonas putida mt-2, which was incorporated with transcriptional activator xylR and fused to aequorin cDNA named pGL2-aequorin. Binding of xylR protein to a subset of toluene-like compounds activates transcription at the Pu promoter, thus expression of aequorin is controlled by xylR and Pu. In this work we have compared the effect of Shine-Dalgarno (SD) and T2 rrnβ terminator sequence in the expression of aequorin. According to the sensitivity of aequorin and increase in the signal-to-noise ratio, this reporter enzyme has reasonable sensitivity compared with other reporter systems. The results indicate higher expression of aequorin in the presence of SD and T2 rrnβ. The activity of aequorin in recombinant whole-cell biosensor was linear from 1 to 500 μm of toluene. The bioluminescence response was specific for toluene-like molecules, so this biosensor cells would be able to detect toluene derivative contamination in environmental samples, accurately.

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The TOL (pWW0) catabolic plasmid

Cited by 116 publications

References 53 publications

Ecological fate, effects and prospects for the elimination of environmental polychlorinated biphenyls (PCBs)

Ecological fate, effects and prospects for the elimination of environmental polychlorinated biphenyls (PCBs)

Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-cresol-degradative strains of Pseudomonas fluorescens

Design and Characterization of an Aequorin‐based Bacterial Biosensor for Detection of Toluene and Related Compounds

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