The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to juvenile carp Cyprinus carpio (L.)

McLean, E.; Ash, R. W.

doi:10.1016/0300-9629(86)90388-9

Cited by 43 publications

(17 citation statements)

References 15 publications

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“…Thus the BSA was apparently present only in the contents of the stomach and not absorbed. Regarding the intestine, there are many studies on the absorption of intact protein (Rombout et al, 1985;Georgopoulou et al, 1986;McLean & Ash, 1986;1987;Suzuki et al, 1988a;1988b;Fujino & Nagai, 1988;Jenkins et al, 1992). Accordingly, intact BSA might be absorbed into the blood through the mucosal epithelium of the posterior intestine after treatment.…”

Section: Discussionmentioning

confidence: 95%

The uptake of bovine serum albumin by the skin of bath-immunised rainbow troutOncorhynchus mykiss

Ototake

Iwama

Nakanishi

1996

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 95%

The uptake of bovine serum albumin by the skin of bath-immunised rainbow troutOncorhynchus mykiss

Ototake

Iwama

Nakanishi

1996

Fish & Shellfish Immunology

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“…Although the mechanism of this transfer is never clarified well, the most logical way is the transfer of the SNV from epithelial cells to phagocytes, like described for transfer of melanin to human skin epithelial cells and apical parts of rodlets to pagocytosing chromatophores in the mammalian retina. The receptor mediated uptake of HRP results in a very fast peak of HRP in the blood of carp [29] and trout [30] 30 min after oral administration, while the appearance of ferritin or LPS inside macrophages is more a process of 4e8 h [7,21]. The HRP receptor is presently characterized, but the biological reason for this receptor mediated uptake of enzymes still not [31].…”

Section: Food and Antigen Uptake In The Gut Of Fishmentioning

confidence: 96%

Teleost intestinal immunology

Rombout¹,

Abelli²,

Picchietti³

et al. 2011

Fish & Shellfish Immunology

500

284

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Teleosts clearly have a more diffuse gut associated lymphoid system, which is morphological and functional clearly different from the mammalian GALT. All immune cells necessary for a local immune response are abundantly present in the gut mucosa of the species studied and local immune responses can be monitored after intestinal immunization. Fish do not produce IgA, but a special mucosal IgM isotype seems to be secreted and may (partly) be the recently described IgZ/IgT. Fish produce a pIgR in their mucosal tissues but it is smaller (2 ILD) than the 4-5 ILD pIgR of higher vertebrates. Whether teleost pIgR is transcytosed and cleaved off in the same way needs further investigation, especially because a secretory component (SC) is only reported in one species. Teleosts also have high numbers of IEL, most of them are CD3-ɛ+/CD8-α+ and have cytotoxic and/or regulatory function. Possibly many of these cells are TCRγδ cells and they may be involved in the oral tolerance induction observed in fish. Innate immune cells can be observed in the teleost gut from first feeding onwards, but B cells appear much later in mucosal compartments compared to systemic sites. Conspicuous is the very early presence of putative T cells or their precursors in the fish gut, which together with the rag-1 expression of intestinal lymphoid cells may be an indication for an extra-thymic development of certain T cells. Teleosts can develop enteritis in their antigen transporting second gut segment and epithelial cells, IEL and eosinophils/basophils seem to play a crucial role in this intestinal inflammation model. Teleost intestine can be exploited for oral vaccination strategies and probiotic immune stimulation. A variety of encapsulation methods, to protect vaccines against degradation in the foregut, are reported with promising results but in most cases they appear not to be cost effective yet. Microbiota in fish are clearly different from terrestrial animals. In the past decade a fast increasing number of papers is dedicated to the oral administration of a variety of probiotics that can have a strong health beneficial effect, but much more attention has to be paid to the immune mechanisms behind these effects. The recent development of gnotobiotic fish models may be very helpful to study the immune effects of microbiota and probiotics in teleosts.

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“…In carp, the rapid appearance of this enzyme in the circulation after oral administration suggests an efficient absorption mechanism [Hertz et al, 1991;McLean and Ash, 1986]. In fact, after 5 min of oral intubation, HRP was readily detected bound to the apical membranes and in apical vesicles of the enterocytes [Rombout et al, 1985].…”

mentioning

confidence: 93%

“…The internalization across the intestinal epithelium of a wide variety of intact proteins (e.g., peroxidase [Rombout et al, 1985;McLean and Ash, 1986;McLean and Ash, 1987]; human, bovine, and salmon growth hormone [Le Bail et al, 1989;Moriyama et al, 1990;Hertz et al, 1991]; insulin [Hertz et al, 1992;Vera et al, 1993]; IgG [Nakamura et al, 1990;Nakamura et al, 1998]; apolipoprotein A-I [Vera et al, 1992]; and lactoferrin [Sakai et al, 1995]) has been demonstrated in several teleost fish species. Most of these studies were designed to demonstrate that after oral or anal administration of several proteins of biologic interest, they could reach the systemic circulation retaining their biologic activities.…”

mentioning

confidence: 99%

Horseradish peroxidase binding to intestinal brush-border membranes ofCyprinus carpio. Identification of a putative receptor

et al. 2000

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Morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (HRP) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. The aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (BBM) to demonstrate and characterize these specific HRP binding sites. The results obtained show that HRP binding to BBM exhibits a saturable mode and high affinity (K(d) = 22 nM). In addition, HRP binding sites are highly enriched in BBM compared to basolateral membranes. On the other hand, HRP interaction with these sites is apparently of an ionic character because binding increased concomitantly with decreasing NaCl concentrations in the assay, reaching a maximum in the absence of NaCl. Other proteins that are also internalized in carp intestine did not significantly inhibit HRP binding to BBM. A lectin-type of interaction was discarded because neither manan nor ovoalbumin inhibited HRP binding. Proteinase K treatment of BBM reduced HRP binding by 70%, suggesting a proteic nature for this binding site. Finally, ligand blotting assays showed that HRP binds specifically to a 15.3-kDa protein. Taken together, these results are consistent with the existence of a functional receptor for HRP in carp intestinal mucosa that could mediate its internalization.

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The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to juvenile carp Cyprinus carpio (L.)

Cited by 43 publications

References 15 publications

The uptake of bovine serum albumin by the skin of bath-immunised rainbow troutOncorhynchus mykiss

The uptake of bovine serum albumin by the skin of bath-immunised rainbow troutOncorhynchus mykiss

Teleost intestinal immunology

Horseradish peroxidase binding to intestinal brush-border membranes ofCyprinus carpio. Identification of a putative receptor

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