The time‐course of acid excretion, levels of fluorescence dependent on cellular nicotinamide adenine nucleotide and glycolytic intermediates of <i>Streptococcus mutans</i> cells exposed and not exposed to air in the presence of glucose and sorbitol

Iwami, Y.; Takahashi-Abbe, S.; Takahashi, Nobutaka; Yamada, Tadashi; Kano, Noriko; Mayanagi, Hideaki

doi:10.1034/j.1399-302x.2001.160106.x

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…In old cultures, cell viability and autofluorescence were lower compared to young cultures (26). A recent a study showed that glucose and sorbitol metabolism could also be measured by changes in NADH fluorescence in Streptococcus mutans (27). These studies might help to understand the relationship between NADH, viability and autofluorescence but data were obtained using vegetative forms of bacteria or eukaryotic cells and spore autofluorescence was only studied in previous Defence Research and Development Canada (DRDC) projects.…”

Section: Discussionmentioning

confidence: 99%

Autofluorescence as a viability marker for detection of bacterial spores

Laflamme

Verreault²,

Lavigne³

et al. 2005

Front Biosci

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Recent biological terrorism events have indicated that bacterial spores such as Bacillus anthracis are real threat agents. Real time detection of biological agents is possible with the use of an ultraviolet Fluorescent Aerodynamic Particle Sizer (FLAPS) that measures particles' intrinsic fluorescence. It is important to know whether intrinsic fluorescence could be used to estimate agents' viability. Two categories of Bacillus spore populations can be differentiated by the intensity of intrinsic fluorescence emitted by ultraviolet (UV) stimulation : autofluorescent and non-autofluorescent. This study was performed to determine whether intensity of autofluorescence correlates with spore viability. Spores were analyzed using flow cytometer (equipped with a cell sorter) to mimic optical properties of FLAPS. Autofluorescent and non-autofluorescent spores were sorted according to the intensity of autofluorescence emitted following UV stimulation. Culturability, membrane integrity, membrane potential and dipicolinic acid (DPA) content were assessed. Autofluorescent spores were 1.7 times more culturable than the corresponding non-autofluorescent population. Moreover, a small proportion of autofluorescent spores exhibited extracellular membrane damages. Autofluorescent spores also showed higher membrane potential activity and contained higher levels of DPA. In conclusion, this study documents that the overall viability potential of bacterial spores can be assessed by UV flow cytometry used in the FLAPS technology.

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Section: Discussionmentioning

confidence: 99%

Autofluorescence as a viability marker for detection of bacterial spores

Laflamme

Verreault²,

Lavigne³

et al. 2005

Front Biosci

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“…Sources that provide light in the region of approximately 350Á370 nm enable detection of reduced pyridine nucleotides (e.g. NAD(P)H) and riboflavin that are biological molecules linked to cellular metabolism (Harrison and Chance, 1970;Eng et al, 1989;Kell et al, 1991;Li et al, 1991;Iwami et al, 2001). Detection of auto-fluorescence under these conditions may indicate the presence of viable biological material in the aerosol particles, although other biological molecules (e.g.…”

Section: Modern Analysis Methodsmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,173

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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“…Glycolytic intermediates were extracted from cells following the method of Iwami et al [2001]. The cells of S. mutans NCTC 10449 were collected by fi ltering 3.0 ml of reaction mixture through a membrane fi lter (pore size 0.45 m; Acrodisc, polyethersulfone, Pall Gelman Laboratory, Ann Arbor, Mich., USA) at 0, 2, 4 and 6 min after the addition of glucose.…”

Section: Determination Of Glycolytic Intermediatesmentioning

confidence: 99%

Synergistic Inhibition by Combination of Fluoride and Xylitol on Glycolysis by Mutans Streptococciand Its Biochemical Mechanism

Maehara¹,

Iwami

Mayanagi³

et al. 2005

Caries Res

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The purpose of this study was to evaluate the combined inhibitory effect of fluoride and xylitol on acid production by mutans streptococci, Streptococcus mutans NCTC10449 and Streptococcus sobrinus 6715, from glucose under strictly anaerobic conditions at fixed pH 5.5 and 7.0. The bacteria were grown in a tryptone-yeast extract broth under strictly anaerobic conditions (N₂: 80%; H₂: 10%; CO₂: 10%). Reaction mixtures for acid production from glucose contained bacterial cells with fluoride (0–6.4 mM) and/or xylitol (60 mM). Acidic end products of glucose fermentation and intracellular glycolytic intermediates were assayed. The combination of fluoride and xylitol inhibited acid production more effectively than fluoride or xylitol alone. In the presence of fluoride and xylitol, the proportion of lactic acid in the total amount of acidic end products decreased, while the proportion of formic and acetic acids increased. Analyses of intracellular glycolytic intermediates revealed that xylitol inhibited the upper part of the glycolytic pathway, while fluoride inhibited the lower part. This study indicates that fluoride and xylitol together have synergistic inhibitory effects on the acid production of mutans streptococci and suggests that xylitol has the potential to enhance inhibitory effects of low concentrations of fluoride.

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The time‐course of acid excretion, levels of fluorescence dependent on cellular nicotinamide adenine nucleotide and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air in the presence of glucose and sorbitol

Cited by 8 publications

References 15 publications

Autofluorescence as a viability marker for detection of bacterial spores

Autofluorescence as a viability marker for detection of bacterial spores

Primary biological aerosol particles in the atmosphere: a review

Synergistic Inhibition by Combination of Fluoride and Xylitol on Glycolysis by Mutans Streptococciand Its Biochemical Mechanism

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