The time-course analysis of gene expression during wound healing in mouse skin

Kagawa, Shinichiro; Matsuo, Aya; Yagi, Yoichi; Ikematsu, Kazuya; Tsuda, Ryouichi; Nakasono, Ichiro

doi:10.1016/j.legalmed.2008.09.004

Cited by 34 publications

(26 citation statements)

References 34 publications

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“…Nowadays, attempt has been made for timing of wound by detection of mRNA [28]. Recently, analysis of mRNA expression in ten genes using real-time PCR implies that genes have differential expressional patterns with time course in mouse skin wound [29]. The time-dependent expression of troponin I mRNA in the contused skeletal muscle of rats was detected by real-time PCR for wound age estimation [30].…”

Section: Discussionmentioning

confidence: 99%

Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice

Fan

et al. 2011

Int J Legal Med

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The study investigated the expression of monoacylglycerol lipase (MGL) during the skin-incised wound healing in mice and applicability of the time-dependent expression of MGL to wound age determination by immunofluorescent staining, Western blotting, and real-time PCR. Furthermore, cell types were identified by double immunofluorescence. A total of 45 BALB/c male mice were used in this study. After a 1.5-cm-long incision in the central dorsum skin, mice were killed at intervals ranging from 6 h to 14 days, followed by the sampling of wound margin. In the control, there was a low-level expression of MGL in the epidermis, hair follicles, and glandulae sebaceae. In the injured skin, MGL immunoreactivity was mainly detected in the neutrophils, macrophages, and myofibroblasts. Morphometrically, the average ratios of MGL-positive cells were more than 50% at 5 and 7 days post-wounding, whereas it was <50% at the other posttraumatic intervals. By Western blotting analysis, the average ratio of MGL protein expression was highest at 5 days after injury, which had a ratio of >2.30. Similarly, the relative quantity of MGL mRNA expression maximized at posttraumatic 5 days in comparison with control as detected by real-time PCR, with an average ratio of >2.54. In conclusion, MGL expression is detected in neutrophils, macrophages, and myofibroblasts and significantly up-regulated, suggesting that it may play roles in response to inflammation during skin-incised wound healing. From the viewpoint of forensic pathology, MGL detection is applicable to skin wound age determination.

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Section: Discussionmentioning

confidence: 99%

Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice

Fan

et al. 2011

Int J Legal Med

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“…Most researches on forensic wound age determination have been focused on human and murine skin wound healing process in which a variety of biological substances are involved [21][22][23][24][25][26]. However, forensic wound age estimation for mammalian skeletal muscle has not been given enough attention yet.…”

Section: Discussionmentioning

confidence: 99%

The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats

Cheng

et al. 2010

Int J Legal Med

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The expression of the cannabinoid receptor type 2 (CB2R) was investigated by immunohistochemistry, Western blotting, and RT-PCR during wound healing of contused skeletal muscle in rats with attempt of its applicability to skeletal muscle wound age estimation. Furthermore, Macrophage Marker (MAC387) was utilized to identify macrophages recruited into injured skeletal muscle tissue. Co-localization of CB2R with Macrophage Marker was detected by confocal laser scanning microscopy. A total of 50 Sprague-Dawley male rats were divided into control and contusion groups (3 h, 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 10 days, and 14 days post-injury). In the uninjured controls, immunoreactivity of CB2R was detected in the sarcolemma and sarcoplasm of normal myofibers. In the contusion groups, a few polymorphonulcear cells, a large number of macrophages, and spindle-shaped fibroblastic cells showed a positive staining for CB2R in wounded zones. By Western blotting analysis, the average of CB2R to GAPDH ratios in 5-7 days post-injury groups was highest, and all the samples had ratios of >2.60. In the other groups, no samples showed ratios of >2.60 and the CB2R to GAPDH ratios ranged from 1.19 to 2.59. The expression tendency was also confirmed by RT-PCR. From the viewpoint of forensic pathology, these observations suggested that the ratio markedly exceeding 2.60 strongly indicated a wound age of 5-7 days. In conclusion, dynamic distribution and expression of CB2R suggest that CB2R be involved in modulating macrophages in response to inflammatory event in rat skeletal muscle wound healing and CB2R be available as a marker for wound age determination.

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“…In the present study, we also performed a comparative analysis of diabetic and healthy individuals using db/db, a well-known type 2 DM model mouse with a defect in leptin receptor (29)(30)(31)that is commonly used in histopathological studies of wound healing (7,17) . On comparative analysis between m+ /m+ and db/db, the results corresponded to previous studies (7,17) , as the process of immune response and tissue repair in db/db was delayed when compared to m+/m+ in both the silver and resin groups.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study on Effects of Silver and Resin Fragments Injected into Periodontal Tissues in Healthy (m + /m +) and Diabetic Model (db/db) Mice with Special Reference to Histopathological and Immunohistochemical Observation

et al. 2013

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High molecular weight compounds have been widely used in the field of dentistry.Although preparation of the tooth abutment and contamination by metal fragments are known to cause discoloration and injury in the periodontal tissues in the field of crown and bridge prosthodontics, the changes due to metal and composite resin fragments have not been fully comparatively studied and clarified to date.In addition, as patients with diabetes are more frequently encountered in dental clinics, the present study conducted histopathological, histochemical and immunohistochemical analyses in order to comparatively elucidate the changes in periodontal tissues after injection of metal(silver group)or composite resin fragments(resin group) . The histopathological reactions of the periodontal tissues in both healthy(m+/m+)and diabetic(db/db)mice were also compared at 28 days after injection.Histopathologically and immunohistochemically, inflammatory cell infiltration and CD68-and MMP-2-positive macrophages were observed around the silver and resin fragments, and the fragments were decreased during fibrosis up to 28 days; these findings were more marked in the resin group than in the silver group. In addition, accumulation of the macrophages and subsequent fibrosis showed a delay in db/db. Furthermore, the number of mast cells was higher degree in the resin group than in the silver group, and was higher in db/db than in m+/m+, indicating that resin induces an allergic reaction in db/db. These results suggest that resin fragments are more readily excised than silver fragments due to the higher level of phagocytosis and antigen-presenting activity of macrophages.Furthermore, critical changes against precipitation were delayed in db/db as compared to m+m+.

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The time-course analysis of gene expression during wound healing in mouse skin

Cited by 34 publications

References 34 publications

Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice

Time-dependent expression and distribution of monoacylglycerol lipase during the skin-incised wound healing in mice

The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats

Comparative Study on Effects of Silver and Resin Fragments Injected into Periodontal Tissues in Healthy (m + /m +) and Diabetic Model (db/db) Mice with Special Reference to Histopathological and Immunohistochemical Observation

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