The tight junction scaffolding protein cingulin regulates cellular delamination from the neuroepithelium

Abstract

Neural crest cells are a transient, multi-potent cell population requisite for vertebrate development. Premigratory neural crest cells exist as adherent epithelial cells and undergo an epithelial-tomesenchymal transition (EMT) to become motile. After cessation of migration, these cells differentiate to form parts of the peripheral nervous system, melanocytes, and the craniofacial skeleton. At the onset of EMT, premigratory neural crest cells lose intercellular contacts mediated by both adherens junctions and tight junctions in order to facilitate emigration of cells out of the dorsal neural tube. We now show that cingulin, a tight junction scaffolding protein, plays an important role in controlling chick midbrain neural crest cell delamination and migration. Overexpression of cingulin enhances emigration of neural crest cells and leads to ectopic delamination of more ventrolateral neuroepithelial cells. Cingulin depletion also augments neural crest cell emigration through the premature and persistent loss of laminin and Cad6B. Our data indicate that cingulin may carry out this function through effects on Rho proteins. Taken together, our results show that cingulin plays a crucial function in the development of the vertebrate embryo through the modulation of neural crest and neuroepithelial cell delamination.The neural crest is a population of migratory cells that follows specific pathways during development, eventually differentiating to form parts of the face, heart, and peripheral nervous system. Stationary, premigratory neural crest cells (NCCs) in the dorsal neural tube transition to migratory NCCs through the loss of cellcell junctions, including tight junctions, during the NCC epithelialto-mesenchymal transiton (EMT). Tight junctions (TJs) are located on the apical side of epithelial cells and serve to maintain cell polarity and to form a barrier that prevents the flow of molecules between the apical and basolateral surfaces of cells. Our data suggest that the TJ transmembrane molecule claudin-1 is a candidate protein that may play a role in NCC development. We have characterized the RNA and protein distribution of claudin-1 at various stages of chick embryonic development, focusing on the midbrain region prior to and after NCC migration. Our results indicate that claudin-1 mRNA is localized to premigratory NCCs in the dorsal neural folds before NCC EMT, and claudin-1 protein is distributed along the apical region of the neural tube. Importantly, claudin-1 transcripts and protein are not observed in migratory NCCs. We will next investigate the effects of claudin-1 depletion and overexpression on NCC EMT and migration. Our experiments will reveal the functional role of claudin-1, and the importance of dismantling TJs in the proper migration of NCCs to build the vertebrate embryo.The development of the lower jaw is regulated spatiotemporally by signaling cascades, and is refined through both permissive and inhibitory signals. We have shown that endothelin-A receptor (Ednra) signaling is a central regulator of...