The tight junction protein JAM-A functions as coreceptor for rotavirus entry into MA104 cells

Torres-Flores, Jesús; Silva‐Ayala, Daniela; Espinoza, Marco A.; López, Susana

doi:10.1016/j.virol.2014.11.016

Cited by 47 publications

(46 citation statements)

References 38 publications

(45 reference statements)

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“…Thus, it is suggested that there is a correlation between the injury of epithelial barrier and damage to tight junction complexes. The tight junction of epithelial cells is a multi-functional complex consisting of many proteins (29,30), including occludin, claudins and actin. As a large protein family, there are a number of claudin subtypes with diverse functions (31,32).…”

Section: Discussionmentioning

confidence: 99%

Establishment and evaluation of an experimental rat model for high-altitude intestinal barrier injury

Luo

Zhou

Chen

et al. 2016

Experimental and Therapeutic Medicine

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In the present study an experimental high-altitude intestinal barrier injury rat model was established by simulating an acute hypoxia environment, to provide an experimental basis to assess the pathogenesis, prevention and treatment of altitude sickness. A total of 70 healthy male Sprague-Dawley rats were divided into two groups: Control group (group C) and a high-altitude hypoxia group (group H). Following 2 days adaptation, the rats in group H were exposed to a simulated 4,000-m, high-altitude hypoxia environment for 3 days to establish the experimental model. To evaluate the model, bacterial translocation, serum lipopolysaccharide level, pathomorphology, ultrastructure and protein expression in rats were assessed. The results indicate that, compared with group C, the rate of bacterial translocation and the apoptotic index of intestinal epithelial cells were significantly higher in group H (P<0.01). Using a light microscope it was determined that the intestinal mucosa was thinner in group H, there were fewer epithelial cells present and the morphology was irregular. Observations with an electron microscope indicated that the intestinal epithelial cells in group H were injured, the spaces among intestinal villi were wider, the tight junctions among cells were open and lanthanum nitrate granules (from the fixing solution) had diffused into the intestinal mesenchyme. The expression of the tight junction protein occludin was also decreased in group H. Therefore, the methods applied in the present study enabled the establishment of a stable, high-altitude intestinal barrier injury model in rats.

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Section: Discussionmentioning

confidence: 99%

Establishment and evaluation of an experimental rat model for high-altitude intestinal barrier injury

Luo

Zhou

Chen

et al. 2016

Experimental and Therapeutic Medicine

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“…Other proposed receptors for rotavirus cell entry include integrins 210 , heat shock protein 70 (REFS 211,212) and junctional proteins such as junctional adhesion molecule A, occludin and tight junction protein ZO-1 (REF. 213). After initial binding, VP7 and the VP5* domain of VP4 can interact with several of these co-receptors, which are concentrated at lipid rafts to mediate viral entry.…”

Section: Figurementioning

confidence: 99%

Rotavirus infection

Crawford

Ramani

Tate

et al. 2017

Nat Rev Dis Primers

524

558

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Rotavirus infections are a leading cause of severe, dehydrating gastroenteritis in children <5 years of age. Despite the global introduction of vaccinations for rotavirus over a decade ago, rotavirus infections still result in >200,000 deaths annually, mostly in low-income countries. Rotavirus primarily infects enterocytes and induces diarrhoea through the destruction of absorptive enterocytes (leading to malabsorption), intestinal secretion stimulated by rotavirus non-structural protein 4 and activation of the enteric nervous system. In addition, rotavirus infections can lead to antigenaemia (which is associated with more severe manifestations of acute gastroenteritis) and viraemia, and rotavirus can replicate in systemic sites, although this is limited. Reinfections with rotavirus are common throughout life, although the disease severity is reduced with repeat infections. The immune correlates of protection against rotavirus reinfection and recovery from infection are poorly understood, although rotavirus-specific immunoglobulin A has a role in both aspects. The management of rotavirus infection focuses on the prevention and treatment of dehydration, although the use of antiviral and anti-emetic drugs can be indicated in some cases.

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“…Second, TJ components can have a direct role in viral entrance. For example, CLDN1 is used as a co-receptor during viral infection to mediate entry in the liver [124][125][126]. During rotavirus infection, JAM-A was shown to function as a co-receptor for virus entrance in epithelial kidney cells [126].…”

Section: Therapeutic Approaches and Caveatsmentioning

confidence: 99%

“…For example, CLDN1 is used as a co-receptor during viral infection to mediate entry in the liver [124][125][126]. During rotavirus infection, JAM-A was shown to function as a co-receptor for virus entrance in epithelial kidney cells [126]. This points toward a possible, yet unstudied, therapeutic approach in which TJ proteins could be targeted to prevent viral entry at the BCSFB during viral infection in the brain (e.g., viral meningitis).…”

Section: Therapeutic Approaches and Caveatsmentioning

confidence: 99%

Clinical implications of leukocyte infiltration at the choroid plexus in (neuro)inflammatory disorders

Demeestere

Libert

Vandenbroucke

2015

Drug Discovery Today

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The tight junction protein JAM-A functions as coreceptor for rotavirus entry into MA104 cells

Cited by 47 publications

References 38 publications

Establishment and evaluation of an experimental rat model for high-altitude intestinal barrier injury

Establishment and evaluation of an experimental rat model for high-altitude intestinal barrier injury

Rotavirus infection

Clinical implications of leukocyte infiltration at the choroid plexus in (neuro)inflammatory disorders

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