The Thyroid Cytotoxic Autoantibody

Forbes, Ian J.; Roitt, Ivan M.; Doniach, Deborah; Solomon, Irene L.

doi:10.1172/jci104579

Cited by 77 publications

(20 citation statements)

References 19 publications

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“…Non-organ-specific Antibodies in Pernicious Anaemia Further, the thyroid antibody detected by immunofluorescence is the one responsible for the cytotoxic action of Hashimoto serum on thyroid tissue cultures (Irvine, 1962;Forbes et al, 1962). It also bears a close relationship to the presence of lymphadenoid thyroid lesions in the mild subclinical forms of focal thyroiditis seen in thyrotoxic and other goitres and in middle-aged women without overt thyroid disease.…”

Section: Thyroid Antibodies In Pernicious Anaemiamentioning

confidence: 99%

Autoimmune Phenomena in Pernicious Anaemia

Doniach¹,

Roitt²,

Taylor³

1963

BMJ

Self Cite

157

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PARACERvICAL NERVE BLOCK MEDICAL JOURNAL was obvious that very substantial relief of pain was obtained in most cases. Each patient was also carefully questioned, but, in order that the results could be judged with the least possible bias, no comment was made to the patient at the time of the injection, save to explain that an examination was to be made. Ten minutes after the injection the result was assessed by asking the patient, " How are the pains ? " without giving any suggestion of 'expected relief. When recorded and analysed the answers indicated " complete" or " very good " relief of pain in 71%, "partial relief " in 25%, and "no relief " in 4%.These results may be compared with those of other workers, which for this purpose are expressed under similar headings, and on a percentage basis to the nearest unit.

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Section: Thyroid Antibodies In Pernicious Anaemiamentioning

confidence: 99%

Autoimmune Phenomena in Pernicious Anaemia

Doniach¹,

Roitt²,

Taylor³

1963

BMJ

Self Cite

157

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“…This antibody is also responsible for toxic effects on thyroid cells in tissue culture. 42 Koffler and Friedman43 found gamma globulin and complement ( C'3) bound to the colloid in this disease. Caspary and Ball44 have reported elevated immunoconglutinin titers in Hashimoto's disease.…”

Section: Circumstantial Evidence For Antibody-mediated Complement Actmentioning

confidence: 84%

Immunoconglutinin in various rheumatic diseases and certain diseases suspected of an autoimmune pathogenesis

Bienenstock

Bloch

1967

Arthritis & Rheumatism

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“…The method used was based on that of Forbes et al [18], Briefly, 5lCr-labelled eye muscle cells or fibroblasts (5 X I05) in 50 pi were incubated with 75 pi of various dilutions of previously heatinactivated patient or normal scrum, and 75 pi fresh normal AB serum (as source of complement) at 37 °C in 5% CO: and 95% air. After 2-20 h of incubation plates were centrifuged and 100 pi aliquots of the supernatant aspirated and their radioactivity counted.…”

Section: Target Cell Preparationmentioning

confidence: 99%

“…T cell activation, in target cell/lymphocyte co-cultures, was assessed as ^-t h y midine incorporation into DNA. HLA-DR antigen expression on OF, EM and thyroid (THY) cells was assessed using a standard immunofluorescence test and a mouse monoclonal antibody against human HLA-DR [17,18].…”

Section: Target Cell Preparationmentioning

confidence: 99%

Mechanisms of Immune Damage in Graves’ Ophthalmopathy

Hiromatsu¹,

Wang²,

Wosu³

et al. 1987

Horm Res

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We have studied the role of immunologically mediated cytotoxicity in the orbital tissue damage of Graves’ ophthalmopathy. Antibody-dependent cell-mediated cytotoxicity (ADCC) against eye muscle (EM) cells and orbital fibroblasts (OF) was demonstrated in a small proportion of patients, all of whom had severe, recent disease. Antibody-mediated (complement-dependent) cytotoxicity against OF was found in only a few patients. No patients showed lysis above background with EM targets. ADCC activity against OF was absorbed by preincubation of serum with thyroid cells, eye muscle cells, and orbital fibroblasts, as well as thyroid, eye muscle and orbital connective tissue membranes. Both EM and OF were able to express class II MHC HLA-DR antigens when stimulated by γ interferon, phytohemagglutinin or activated T lymphocytes. DR-positive target cells were much more susceptible to lysis, in both ADCC and lymphocyte-mediated cytotoxicity, than DR negative cells. When DR-positive OF and EM were used as targets in ADCC assays, the degree of lysis determined as ⁵¹Cr release given by serum from patients with Graves’ ophthalmopathy was enhanced, but only in those patients showing positive tests with DR-negative targets. Intrathyroidal T lymphocytes obtained from a patient with Graves’ ophthalmopathy were more cytotoxic against DR-positive OF and EM than equal numbers of her peripheral blood T lymphocytes. Antibody-dependent cell-mediated cytotoxicity and lymphocyte-mediated cytotoxicity against orbital fibroblasts and eye muscle cells are thus associated with target cell HLA-DR antigen expression and are likely to be mechanisms for in vivo tissue damage in Graves’ ophthalmopathy. The identity of the mononuclear cell subpopulation effecting cell-mediated cytotoxicity against orbital target cells, and the possible significance of reaction of cytotoxic antibodies against orbital, thyroid-shared antigens are unclear.

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The Thyroid Cytotoxic Autoantibody

Cited by 77 publications

References 19 publications

Autoimmune Phenomena in Pernicious Anaemia

Autoimmune Phenomena in Pernicious Anaemia

Immunoconglutinin in various rheumatic diseases and certain diseases suspected of an autoimmune pathogenesis

Mechanisms of Immune Damage in Graves’ Ophthalmopathy

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The Thyroid Cytotoxic Autoantibody

Cited by 77 publications

References 19 publications

Autoimmune Phenomena in Pernicious Anaemia

Autoimmune Phenomena in Pernicious Anaemia

Immunoconglutinin in various rheumatic diseases and certain diseases suspected of an autoimmune pathogenesis

Mechanisms of Immune Damage in Graves&rsquo; Ophthalmopathy

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Product

Resources

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Mechanisms of Immune Damage in Graves’ Ophthalmopathy