The three‐dimensional nanofiber scaffold culture condition improves viability and function of islets

Abstract

Significant problems existing in the islet transplantation include a poor survival ability of the islet cells cultured under static conditions in vitro, decreased secretion function, and limited transplantation efficiency. In this study, we cocultured the three-dimensional (3D) self-assembling peptide nanofiber hydrogel scaffold with the islets from adult Wistar rats. The nanofiber scaffold constructed a 3D environment for the islets culture. The results of DTZ staining showed that the purity of the islets in the scaffold was >80%. The result of the fluorescent staining with AO-PI demonstrated that the viability of the islets in the 3D culture environment (within scaffold) was greater than those in the two-dimensional (2D) culture environment (without scaffold). The islets encapsulated in the 3D peptide nanofiber scaffold exhibited better secretion function. The insulin releasing index in the 3D group was remarkably higher than that in the 2D group. By scanning electron microscopy, it was observed that the 3D self-assembling peptide nanofiber hydrogel scaffold formed a nano scale fiber with a geometric form and the islets were encapsulated in this scaffold. Our research demonstrated that this nanofiber scaffold provided a favorable 3D environment for the islets to be cultured in vitro and then improve the secretion function and prolong the survival time of the islet in vitro.

Islet transplantation in rodents: do encapsulated islets really work?

“…This permits the imitation of moment-to-moment fine regulation of the missing therapeutic factors, avoids a lifetime of immunosuppressive therapy and allows the use of non-human cells, thus overcoming the limited supply of human donor cells (65) . Zhao et al (64) have demonstrated that encapsulated islets cultured in 3D peptide nanofiber provides a superior simulated microenvironment for improving the viability and the secretion function of the islets.…”

Prospects of Application of Tissue-Engineered Pancreatic Constructs in the Treatment of Type 1 Diabetes

“…Путем поддержания клеток в физиологически более подходящих структурах и сохранения клеточ-но-клеточных и клеточно-матриксных контактов Таблица Краткое описание основных типов тканеинженерных конструкций [11] Summary of types of tissue-engineering constructs [ Островки, культивированные в пористых каркасах, имеют более высокую жизнеспособность и инсулинпродуцирующую активность по сравнению с 2D-культурами [53][54][55]. Включение в каркас молекул ВКМ усиливает секрецию инсулина [53].…”

“…Включение в каркас молекул ВКМ усиливает секрецию инсулина [53]. Островки, имплантированные in vivo в скаффолды, васкуляризируются и снижают уровень гликемии [55] Инкубация с гидрогелем Добавление молекул ВКМ Островки человека и крыс [54,56]. Прогениторные клетки ПЖ крысы [57].…”

“…Совместное культивирование островков взрос-лых крыс Wistar и трехмерного тонковолокнистого гидрогелевого скаффолда показало, что выживае-мость и инсулинсекретирующая активность ОК, не-посредственно контактировавших со скаффолдом, были выше, чем при его отсутствии [54].…”

Tissue Engineering for Tissue and Organ Regeneration

“…The nanofiber scaffold simulated the microenvironment in vitro as in native condition which accounted for improved islet viability and function (Zhao et al, 2010). …”