The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System

Rowe, Cassie J.; Crowley-Perry, Mikayla; McCarthy, Elizabeth; Davidson, Terry L.; Connaughton, Victoria P.

doi:10.3791/61934

Cited by 4 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fish were tested in the three-chamber choice task every 4 weeks ( Figure 12 ), after the onset of treatment, to assess their cognitive abilities [ 26 ]. At the 8-week and 12-week timepoints, visual discrimination was also tested using an optomotor response (OMR).…”

Section: Methodsmentioning

confidence: 99%

“…The behavioral chamber and experimental set up can be found in [ 26 ] and are only briefly described here. The chamber was made from a 40 L aquarium (50 × 30 × 30 cm 3 ).…”

Section: Methodsmentioning

confidence: 99%

“…During group acclimation, up to six zebrafish were tested simultaneously. For acclimation, both side chambers had neutral (beige-colored) backgrounds and a clear container holding four conspecific zebrafish to act as the shoal reward [ 26 ]. The walls of the center chamber were not colored.…”

Section: Methodsmentioning

confidence: 99%

“…Discrimination ratios [ 26 ] were calculated by dividing the number of correct trials by the total number of trials (8) per fish, which was then averaged out over the group. While similar to the percent correct in that both are fractions with 1.00 = 100% and 0.5 = 50%, the discrimination ratios provided a better analysis of individual fish responses.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio

McCarthy

Dunn

Augustine

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The present study induced prolonged hyperglycemia (a hallmark symptom of Type 2 diabetes [T2DM]) in Danio rerio (zebrafish) for eight or twelve weeks. The goal of this research was to study cognitive decline as well as vision loss in hyperglycemic zebrafish. Fish were submerged in glucose for eight or twelve weeks, after which they were assessed with both a cognitive assay (three-chamber choice) and a visual assay (optomotor response (OMR)). Zebrafish were also studied during recovery from hyperglycemia. Here, fish were removed from the hyperglycemic environment for 4 weeks after either 4 or 8 weeks in glucose, and cognition and vision was again assessed. The 8- and 12-week cognitive results revealed that water-treated fish showed evidence of learning while glucose- and mannitol-treated fish did not within the three-day testing period. OMR results identified an osmotic effect with glucose-treated fish having significantly fewer positive rotations than water-treated fish but comparable rotations to mannitol-treated fish. The 8- and 12-week recovery results showed that 4 weeks was not enough time to fully recovery from the hyperglycemic insult sustained.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The behavioral chamber and experimental set up can be found in [ 26 ] and are only briefly described here. The chamber was made from a 40 L aquarium (50 × 30 × 30 cm 3 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio

McCarthy

Dunn

Augustine

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The genetic tractability, high fecundity, and optical transparency of zebrafish also make them excellent tools for investigating human disorders. Thus, zebrafish larvae have recently become an emerging behavior model in determining inner ear relay sensory transduction of hearing and balance [ 6 ]. Accumulating studies have indicated that the ototoxic action of aminoglycosides is mainly due to reactive oxygen species (ROS) generation, which exacerbates oxidative stress and is strongly implicated in the apoptosis and necrosis of hair cells [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Lai

Cheng

et al. 2022

IJMS

View full text Add to dashboard Cite

Ototoxic hearing loss due to antibiotic medication including aminoglycosides and excess free radical production causes irreversible hair cell injury. Cichoric acid, a naturally occurring phenolic acid, has recently been found to exert anti-oxidative and anti-inflammatory properties through its free radical scavenging capacity. The present study aimed to investigate the protective effects of cichoric acid against neomycin-induced ototoxicity using transgenic zebrafish (pvalb3b: TagGFP). Our results indicated that cichoric acid in concentrations up to 5 μM did not affect zebrafish viability during the 2 h treatment period. Therefore, the otoprotective concentration of cichoric acid was identified as 5 μM under 2 h treatment by counting viable hair cells within the neuromasts of the anterior- and posterior-lateral lines in the study. Pretreatment of transgenic zebrafish with 5 μM of cichoric acid for 2 h significantly protected against neomycin-induced hair cell death. Protection mediated by cichoric acid was, however, lost over time. A terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and FM4-64 staining, respectively, provided in situ evidence that cichoric acid ameliorated apoptotic signals and mechanotransduction machinery impairment caused by neomycin. A fish locomotor test (distance move, velocity, and rotation frequency) assessing behavioral alteration after ototoxic damage revealed rescue due to cichoric acid pretreatment before neomycin exposure. These findings suggest that cichoric acid in 5 μM under 2 h treatment has antioxidant effects and can attenuate neomycin-induced hair cell death in neuromasts. Although cichoric acid offered otoprotection, there is only a small difference between pharmacological and toxic concentrations, and hence cichoric acid can be considered a rather prototypical compound for the development of safer otoprotective compounds.

show abstract

Time dependent effects of prolonged hyperglycemia in zebrafish brain and retina

Rowe¹,

Delbridge‐Perry²,

Bonan³

et al. 2022

Front. Ophthalmol.

Self Cite

View full text Add to dashboard Cite

Prolonged hyperglycemia causes long-term vision complications and an increased risk of cognitive deficits. High blood sugar also confers an osmotic load/stress to cells. We assessed behavioral and neurochemical changes in zebrafish brain and retina following prolonged hyperglycemia for 4-weeks or 8-weeks. At each time point, behavior was assessed using 3-chamber choice task and optomotor response; tissue was then collected and levels of inflammatory markers, tight junction proteins, and neurotransmitters determined using Western Blots. After 4-weeks, brain levels of v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RelA; NF-kB subunit), IkB kinase (IKK), and glial fibrillary acidic protein (GFAP) were significantly elevated; differences in zonula occludens-1 (ZO-1), claudin-5, glutamic acid decarboxylase (GAD), and tyrosine hydroxylase (TH) were not significant. In retina, significant differences were observed only for TH (decreased), Rel A (increased), and GFAP (increased) levels. Glucose-specific differences in initial choice latency and discrimination ratios were also observed. After 8-weeks, RelA, GAD, and TH were significantly elevated in both tissues; IKK and GFAP levels were also elevated, though not significantly. ZO-1 and claudin-5 levels osmotically decreased in retina but displayed an increasing trend in glucose-treated brains. Differences in discrimination ratio were driven by osmotic load. OMRs increased in glucose-treated fish at both ages. In vivo analysis of retinal vasculature suggested thicker vessels after 4-weeks, but thinner vessels at 8-weeks. In vitro, glucose treatment reduced formation of nodes and meshes in 3B-11 endothelial cells, suggesting a reduced ability to form a vascular network. Overall, hyperglycemia triggered a strong inflammatory response causing initial trending changes in tight junction and neuronal markers. Most differences after 4-weeks of exposure were observed in glucose-treated fish suggesting effects on glucose metabolism independent of osmotic load. After 8-weeks, the inflammatory response remained and glucose-specific effects on neurotransmitter markers were observed. Osmotic differences impacted cognitive behavior and retinal protein levels; protein levels in brain displayed glucose-driven changes. Thus, we not only observed differential sensitivities of retina and brain to glucose-insult, but also different cellular responses, suggesting hyperglycemia causes complex effects at the cellular level and/or that zebrafish are able to compensate for the continued high blood glucose levels.

show abstract

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System

Cited by 4 publications

References 0 publications

Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio

Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Time dependent effects of prolonged hyperglycemia in zebrafish brain and retina

Contact Info

Product

Resources

About