The ThioredoxinT and deadhead gene pair encode testis- and ovary-specific thioredoxins in Drosophila melanogaster

Svensson, Malin J.; Chen, J. Don; Pirrotta, Vincenzo; Larsson, Jonas

doi:10.1007/s00412-003-0253-5

Cited by 47 publications

(56 citation statements)

References 48 publications

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“…The primer pair 5Ј-CCACCATCTGCGACATA-AACAG-3Ј and 5Ј-CGACGAGTAATTTGGTACACTG-3Ј will amplify only the endogenous Pof transcript, 5Ј-CCACC-ATCTGCGACATAAACAG-3Ј and 5Ј-CGTCGCCGTCCA-GCTCGACCA-3Ј only the Pof.EYFP transcript, and 5Ј-AAA-GACGATGCCAAGGACTCAC-3Ј and 5Ј-GCTTGGCGGCC-GTGCCGACA-3Ј will amplify the neighboring gene CG4806. Poly(A) ϩ RNA was isolated as described (28). One microliter of poly(A) ϩ RNA, pd(T) [12][13][14][15][16][17][18] first-strand primer, primer pairs indicated above, and Ready-to-Go RT-PCR beads (Amersham Biosciences, Piscataway, NJ) was used in each RT-PCR.…”

Section: Methodsmentioning

confidence: 99%

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Larsson

Svensson

Stenberg

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Painting of fourth (POF) is a chromosome-specific protein in Drosophila and represents the first example of an autosome-specific protein. POF binds to chromosome 4 in Drosophila melanogaster, initiating at the proximal region, followed by a spreading dependent on chromosome 4-specific sequences or structures. Chromosome-specific gene regulation is known thus far only as a mechanism to equalize the transcriptional activity of the single male X chromosome with that of the two female X chromosomes. In Drosophila, a complex including the male-specific lethal proteins, ''paints'' the male X chromosome, mediating its hypertranscription, explained to some extent by the acetylation of lysine 16 on histone H4. Here, we show that Pof is essential for viability in both sexes and for female fertility. POF binding to an autosome, the F element, is conserved in genus Drosophila, indicating functional conservation of the autosome specificity. In three of nine studied species, POF binds to the male X chromosome. When bound to the male X, it also colocalizes with the dosage compensation protein male-specific lethal 3, suggesting a relationship to dosage compensation. The chromosome specificity is determined at the species level and not by the amino acid sequence. We argue that POF is involved in a chromosome-specific regulatory function.

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Section: Methodsmentioning

confidence: 99%

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Larsson

Svensson

Stenberg

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…The microarray analysis indicated that transcripts of TrxT, a testis specific isoform of thioredoxin (Svensson et al, 2003), (Fig. 7F).…”

Section: Differential Requirements For Topi and Achi/vis For Expressimentioning

confidence: 99%

“…Another feature these genes have in common is that they are members of clusters of related genes, with different expression patterns. TrxT is found adjacent in the Development 131 (8) Research article genome to the female specific thioredoxin, deadhead (Svensson et al, 2003). CG8349 is the 3′-most of a cluster of three predicted cytidine deaminase genes (the others being CG8360 and CG8353).…”

Section: Structure Of Target Promotersmentioning

confidence: 99%

Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger proteinmatotopetli

et al. 2004

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A robust developmentally regulated and cell type specific transcriptional programme is activated in primary spermatocytes in preparation for differentiation of the male gametes during spermatogenesis. Work in Drosophila is beginning to reveal the genetic networks that regulate this gene expression. The Drosophila aly-class meiotic arrest loci are essential for activation of transcription of many differentiation-specific genes, as well as several genes important for meiotic cell cycle progression,thus linking meiotic cell cycle progression to cellular differentiation during spermatogenesis. The three previously described aly-class proteins(aly, comr and achi/vis) form a complex and are associated with chromatin in primary spermatocytes. We identify, clone and characterize a new aly-class meiotic arrest gene, matotopetli (topi), which encodes a testis-specific Zn-finger protein that physically interacts with Comr. The topimutant phenotype is most like achi/vis in that topi function is not required for the nuclear localization of Aly or Comr, but is required for their accumulation on chromatin. Most target genes in the transcriptional programme depend on both topi and achi/vis; however, a small subset of target genes are differentially sensitive to loss of topior achi/vis, suggesting that these aly-class predicted DNA binding proteins can act independently in some contexts.

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“…In Drosophila, out of 11 predicted Trxs, 3 classical Trx genes have been identified (deadhead [dhd], Trx-2, and TrxT), and mutants for all 3 have been characterized. The dhd and trxT genes form a cluster on the X-chromosome, and both appear to be germ-line specific, dhd being associated with the nurse cell nuclei and karyosomes of the ovary, and trxT with the spermatocytes (111). While Trx-2 has been colocalized with Trx-T, it is also expressed at significant levels in somatic tissues, and thus represents an attractive target for the evaluation of longevity effects.…”

Section: Thioredoxinsmentioning

confidence: 99%

Involvement of Redox State in the Aging ofDrosophila melanogaster

Orr

Radyuk

Sohal

2013

Antioxidants & Redox Signaling

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Significance: The main objective of this review was to provide an exposition of investigations, conducted in Drosophila melanogaster, on the role of reactive oxygen species and redox state in the aging process. While early transgenic studies did not clearly support the validity of the oxidative stress hypothesis of aging, predicated on the accumulation of structural damage, they spawned a broader search for redox-related effects that might impact the aging process. Recent Advances: Initial evidence implicating the thiol redox state as a possible causative factor in aging has been obtained in Drosophila. Overexpression of genes, such as GCL, G6PD, Prx2, and Prx5, which are involved in the maintenance of thiol redox homeostasis, has strong positive effects on longevity. Further, the depletion of peroxiredoxin activity in the mitochondria through the double knockdown of Prx5 and Prx3 not only results in a redox crisis but also elicits a rapid aging phenotype. Critical Issues: Herein, we summarize the present status of knowledge about the main components of the machinery controlling thiol redox homeostasis and describe how age-related redox fluctuations might impact aging more acutely through disruption of the redox-sensitive signaling mechanisms rather than via the simple accumulation of structural damage. Future Directions: Based on these initial insights into the plausible impact of redox fluctuations on redox signaling, future studies should focus on the pathways that have been explicitly implicated in aging, such as insulin signaling, TOR, and JNK/FOXO, with particular attention to elements that are redox sensitive. Antioxid. Redox Signal. 19, 788-803. Historical BackgroundLinkage between energy utilization and life span S tudies in the fruit fly, Drosophila melanogaster, have historically made important contributions toward the development of basic concepts in many areas of biology, including the aging process, especially in defining the role of oxygen metabolism in the determination of life span. In 1917, Loeb and Northrop showed that the life span of D. melanogaster is inversely associated with the ambient temperature (61), which, in turn, was later demonstrated to be linked to the rate of oxygen consumption within the physiological range, whose upper limit for long-term survival and optimal fecundity is *27°C (67). A major advance in the field of aging that can be largely credited to the Drosophila model was Pearl's (77) interpretation of the effects of temperature on longevity, namely, shorter life spans at elevated temperatures are ascribable to a relatively higher physical activity or the rate of living (ROL). As originally proposed, the length of life of an individual organism is governed by two distinct factors: (i) a genetically determined metabolic potential, that is, the total amount of energy consumed during life, which varies in different genotypes, and (ii) the rate at which this metabolic potential is expended by the individual or the rate of its metabolism. It is a widely recognized phe...

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The ThioredoxinT and deadhead gene pair encode testis- and ovary-specific thioredoxins in Drosophila melanogaster

Cited by 47 publications

References 48 publications

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Painting of fourth in genus Drosophila suggests autosome-specific gene regulation

Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger proteinmatotopetli

Involvement of Redox State in the Aging ofDrosophila melanogaster

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