The therapeutic potential of cystathionine gamma-lyase in temporomandibular inflammation-induced orofacial hypernociception

Santos, Bruna M.; Garattini, Emanuela G.; Branco, Luiz G.S.; Leite–Panissi, Christie Ramos Andrade; Nascimento, Glauce Crivelaro do

doi:10.1016/j.physbeh.2018.02.007

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…In colon cancer cell lines, AOAA treatment inhibits H 2 S production, cell migration and cell growth, closely mimicking the effect of CBS silencing 9 19. In vivo, AOAA exhibited remarkable inhibition of HCT116 cell xenografts19 and the proliferation of patient-derived colon tumour xenografts 20. However, since H 2 S is very susceptible to pH changes and can easily be degraded by thiol methyltransferase and rhodanase, the traditional detection assays, such as the methylene blue method, the monobromobimane method and sulfide selective electrodes, cannot achieve a high repeatable conclusion in the diagnosis of CRC.…”

Section: Discussionmentioning

confidence: 85%

“… 9 19 In vivo, AOAA exhibited remarkable inhibition of HCT116 cell xenografts 19 and the proliferation of patient-derived colon tumour xenografts. 20 However, since H 2 S is very susceptible to pH changes and can easily be degraded by thiol methyltransferase and rhodanase, the traditional detection assays, such as the methylene blue method, the monobromobimane method and sulfide selective electrodes, cannot achieve a high repeatable conclusion in the diagnosis of CRC. Compared with detections of H 2 S in human peripheral blood and faeces, exhaled H 2 S is a more reliable, sensitive and accurate detection method.…”

Section: Discussionmentioning

confidence: 99%

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Role of exhaled hydrogen sulfide in the diagnosis of colorectal cancer

Du,

Tseng,

Liu

et al. 2024

BMJ Open Gastroenterol

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BackgroundColorectal cancer (CRC) is often accompanied by increased excretion of hydrogen sulfide (H2S). This study aimed to explore the value of exhaled H2S in the diagnosis of CRC.MethodsA total of 80 people with normal colonoscopy results and 57 patients with CRC were enrolled into the present observational cohort study. Exhaled oral and nasal H2S were detected by Nanocoulomb breath analyser. Results were compared between the two groups. Receiver operating characteristic (ROC) curves were analysed and area under the curves (AUCs) were calculated to assess the diagnostic value of exhaled H2S. Meanwhile, the clinicopathological features, including gender, lesion location and tumour staging of patients with CRC, were also collected and analysed.ResultsThe amount of exhaled H2S from patients with CRC was significantly higher than that of those with normal colonoscopy results. The ROC curve showed an AUC value of 0.73 and 0.71 based on oral and nasal H2S detection, respectively. The exhaled H2S in patients with CRC was correlated with gender, lesion location and tumour progression, including depth of invasion, lymphatic metastasis and TNM (Tumor, Lymph Nodes, Metastasis) staging.ConclusionExhaled H2S analysis is a convenient and non-invasive detection method for diagnosing CRC, suggesting a potential role in population screening for CRC.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Role of exhaled hydrogen sulfide in the diagnosis of colorectal cancer

Du,

Tseng,

Liu

et al. 2024

BMJ Open Gastroenterol

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“…The application of CFA into different sites has been widely used to induce inflammation and to characterize the mechanisms implicated in inflammatory pain [ 8 – 12 ]. However, only a few studies investigated the outcomes after the application of CFA into the rat dental pulp [13–16;22].…”

Section: Discussionmentioning

confidence: 99%

“…Complete Freund’s adjuvant (CFA) is a mixture of mineral oils containing heat-inactivated Mycobacterium tuberculosis , which has been widely used to induce chronic inflammatory pain when injected into the rat hind paw or knee joint [ 7 , 8 ]. This pro-inflammatory agent has also been used to study orofacial pain, after injection into the temporomandibular joint (TMJ) or perioral skin [ 9 – 12 ]. A few studies have also analyzed the effects of CFA application into the rat tooth pulp.…”

Section: Introductionmentioning

confidence: 99%

Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats

et al. 2018

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This study further investigated the mechanisms underlying the rat model of tooth pulp inflammatory pain elicited by complete Freund’s adjuvant (CFA), in comparison to other pulpitis models. Pulps of the left maxillary first molars were accessed. In the CFA group, the pulps were exposed, and CFA application was followed by dental sealing. In the open group, the pulps were left exposed to the oral cavity. For the closed group, the pulps were exposed, and the teeth were immediately sealed. Naïve rats were used as negative controls. Several parameters were evaluated at 1, 2, 3 and 8 days. There was no statistical significant difference among the groups when body weight variation, food or water consumption were compared. Analysis of serum cytokines (IL-1β, TNF or IL-6) or differential blood cell counts did not reveal any evidence of systemic inflammation. The CFA group displayed a significant reduction in the locomotor activity (at 1 and 3 days), associated with an increased activation of satellite glial cells in the ipsilateral trigeminal ganglion (TG; for up to 8 days). Amygdala astrocyte activation was unaffected in any experimental groups. We provide novel evidence indicating that CFA-induced pulp inflammation impaired the locomotor activity, with persistent activation of ipsilateral TG satellite cells surrounding sensory neurons, without any evidence of systemic inflammation or amygdala astrogliosis.

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“…No caso da dor crônica orofacial, foi observado que a expressão de CBS no gânglio trigeminal está aumentada, indicando seu papel pró-nociceptivo na dor orofacial crônica (MIAO et al, 2014). Dados de nosso laboratório ainda mostraram que o inibidor de H2S na região orofacial atenua a resposta nociceptiva na dor persistente (SANTOS et al, 2018), sendo esta atenuação dependente da diminuída interação neuroimune facilitando a resposta dolorosa na região orofacial (GARATTINI et al, 2019).…”

Section: Sulfeto De Hidrogênio (H2s)unclassified

Efeito do sulfeto de hidrogênio hipotalâmico modulando a tolerância ao LPS

Santos¹

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The therapeutic potential of cystathionine gamma-lyase in temporomandibular inflammation-induced orofacial hypernociception

Cited by 13 publications

References 32 publications

Role of exhaled hydrogen sulfide in the diagnosis of colorectal cancer

Role of exhaled hydrogen sulfide in the diagnosis of colorectal cancer

Activation of trigeminal ganglion satellite glial cells in CFA-induced tooth pulp pain in rats

Efeito do sulfeto de hidrogênio hipotalâmico modulando a tolerância ao LPS

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