The theory of turbines

Thurston, R. H.

doi:10.1016/0016-0032(84)90361-2

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“…Consequently, elimination of the POFl allele from Dex+ strains, by conventional methods of yeast breeding, produced Pof" strains which were found to be suitable for the construction of hybrids with brewers' yeast. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] Since the Pof" phenotype is a distinguishing characteristic of brewers' yeast, it is of some interest to extend the analysis to the molecular genetic level. This study was greatly facilitated by the finding that the decarboxylasc produced by Pof* strains of Sacch.…”

Section: Introductionmentioning

confidence: 99%

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Cloning of a Yeast Gene Which Causes Phenolic Off-Flavours in Beer

Meaden

Taylor²

1991

Journal of the Institute of Brewing

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A gene {POFl) has been cloned, which confers upon yeast {Saccharomyces cerevisiae) the ability to decarboxylate phenolic acids such as ferulic and trans-cinnamic acid. This property was previously shown to be a cause of phenolic off-flavour production in wort fermentations. The identity of the cloned gene was confirmed as POF1 by gene disruption techniques. Southern blotting of total genomic DNA revealed that sequences homologous to POF1 are conserved in Pof" brewing strains of Sacch. cerevisiae. The transformation of a Pof" lager strain with the cloned POF1 gene led to the production of an aroma characteristic of a phenolic off-flavour, when the transformed strain was used in wort fermentations. This latter observation suggests that the Pof~phenotype of brewers' yeast is specifically due to the absence of a functional POF1 gene.

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Section: Introductionmentioning

confidence: 99%

“…cerevisiae can convert frans-cinnamic acid to styrene. 19 Consequently, Pof+ strains grown in wort or other complex media supplemented with /ra/is-cinnamic acid will produce a distinctive odour, which is lacking from cultures of Pof" strains (e.g. brewers' yeast).…”

Section: Introductionmentioning

confidence: 99%

Cloning of a Yeast Gene Which Causes Phenolic Off-Flavours in Beer

Meaden

Taylor²

1991

Journal of the Institute of Brewing

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show abstract

The theory of turbines

Cited by 1 publication

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Cloning of a Yeast Gene Which Causes Phenolic Off-Flavours in Beer

Cloning of a Yeast Gene Which Causes Phenolic Off-Flavours in Beer

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