The theory of phase separation in an non-concentrated solution of several polydispersed polymers

Shishov, A.K.; Frenkel, S.Ya.

doi:10.1016/0032-3950(78)90481-1

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…With these parameters the UNIQUAC model led to good phase behavior predictions for Dx/ PEG/water systems of various molecular weights. 16 Thus the UNIQUAC model with parameters listed in Table II was used in the LLE calculations here.…”

Section: Quadrature Methodsmentioning

confidence: 99%

Effects of polydispersivity on the phase behavior of the aqueous two-phase polymer systems

Kang¹,

Sandler²

1988

Macromolecules

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Section: Quadrature Methodsmentioning

confidence: 99%

Effects of polydispersivity on the phase behavior of the aqueous two-phase polymer systems

Kang¹,

Sandler²

1988

Macromolecules

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“…Vink (1976), Shiskov and Frenkel (1979), and Shiskov et al (1981) have suggested that truncation of the osmotic virial equation after the third virial coefficient may be more accurate. This suggestion has been verified by Haynes et al (1988).…”

Section: October 1988mentioning

confidence: 98%

Molecular thermodynamics of aqueous two‐phase systems for bioseparations

1988

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Aqueous polymer-polymer two-phase systems provide a powerful method for separating biomolecules by extraction. When a complex mixture of biomolecules (e.g., a fermentation broth or a solution of lysed cells) is added to such a system, biomolecules partition uniquely between the two phases, achieving separation.A thermodynamic framework is presented for optimizing extraction performance in biological separations. First, a rnolecular-thermodynamic model, based on the osmotic virial equation, is proposed to describe phase equilibria for dilute aqueous mixtures containing polymers and protein. Second, experimental phase-equilibrium data (protein partition coefficients) are reported for a number of model proteins including albumin, lysozyme, and a-chymotrypsin. To interpret and correlate the experimental data, Low-Angle Laser-Light Scattering (LALLS) measurements were made to determine osmotic second virial coefficients for aqueous mixtures containing polymers, proteins, salts (KCI, KH,PO, and K,S04 at concentrations of 50 and 100 mM) and several combinations of polymer-polymer and polymer-protein pairs. Combined with electrochemical measurements (differences in potential between the two phases and protein net charge), these data provide parameters for the model to calculate the desired phase equilibria. A comparison of calculated and experimental results indicates that the virial-equation model provides good prediction of binodals and a reliable basis for estimating infinite-dilution protein partition coefficients for biotechnical process design.

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