The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells

Ludwig, Antje; Lorenz, Mario; Grimbo, Nicole; Steinle, Frederike; Meiners, Silke; Bartsch, Cornelia; Stangl, Karl; Baumann, Gert; Stangl, Verena

doi:10.1016/j.bbrc.2004.02.099

Cited by 183 publications

(125 citation statements)

References 33 publications

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“…[41][42][43] In addition, several types of natural antioxidants, including catechins, flavonoids, and related polyphenolic compounds, have been shown to inhibit adhesion molecule expression and the adhesion of monocytes to endothelial cells. 44,45) Previous studies have shown that CA is effective in protecting U937 against tert-butyl hydroperoxide-induced oxidative damage both in vivo and in vitro. 46) Therefore, the inhibitory effect of CA on the TNF-a-induced activation of NF-kB might be due to its antioxidant properties.…”

Section: Discussionmentioning

confidence: 99%

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Moon

Lee

Kim

et al. 2009

Biological & Pharmaceutical Bulletin

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Recruitment of specific leukocyte subpopulations at the site of inflammation requires a series of cell adhesion molecules (CAMs)-mediated interactions. The major CAMs, viz., intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin are expressed on endothelium in response to various cytokines. Caffeic acid (CA), a natural phenolic compound from herbs and other sources, has been shown to prevent cardiovascular diseases. We investigated the effect of CA on the expression of CAMs by human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor (TNF-a a). Adhesion of monocytes to CAtreated HUVECs was evaluated by co-culture experiments using 2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethylester (BCECF-AM) labeling of U937 cells. The expression of adhesion and chemoattractant molecules was evaluated by Western blot and reverse transcription-polymerase chain reaction (RT-PCR), respectively. CA significantly inhibited the TNF-a a-induced increase in U937 monocyte adhesion to HUVECs as well as decreased the protein and mRNA expression levels of CAMs on HUVECs. CA also inhibited the mRNA expression of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). The involvement of nuclear factor (NF)-k kB in the transcriptional control of CAMs protein was assessed by degradation of inhibitory (I)k kB and nuclear translocation of NF-k kB using Western blotting and immunofluorescence staining. CA attenuated TNF-a ainduced Ik kB degradation and NF-k kB translocation from cytosol to the nucleus. In conclusion, TNF-a a-induced NF-k kB-DNA complex formation was inhibited by CA. CA reduced TNF-a a-induced endothelial adhesiveness to HUVECs by inhibiting transcription factor activation, and CAMs expression suggesting its potential role in atherosclerosis diseases.

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Section: Discussionmentioning

confidence: 99%

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Moon

Lee

Kim

et al. 2009

Biological & Pharmaceutical Bulletin

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“…EGCG, a polyphenolic compound from green tea, has antioxidative, antithrombogenic, antiallergic, and anti-inflammatory properties. Its inhibitory action on cell adhesion has previously been studied in the prevention of atherosclerosis and cancer metastasis (Hung et al, 2005;Ludwig et al, 2004;Zhang et al, 2000). The interaction of EGCG with fibronectin and laminin was also suggested as a mechanism (Sazuka et al, 1998;Suzuki and Isemura, 2001).…”

Section: Discussionmentioning

confidence: 99%

Albumin and Antioxidants Inhibit Serum-deprivation-induced Cell Adhesion in Hematopoietic Cells

Han¹,

Lim²,

Kim³

et al. 2008

Biomolecules and Therapeutics

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− Previously, we identified albumin as an inhibitory factor in serum for cell adhesion of T cells such as human Jurkat T and primary cultured human T cells. In the present study, we found that other hematopoietic cell lines including U-937 human monocytes, THP-1 human monocytes, K-562 promyelocytic leukemia cells, and HL-60 human leukemia cells, also adhere to tissue culture flasks when serum is withdrawn, and albumin exerts an inhibitory effect on cell adhesion by those cells, implying that this inhibition is a common phenomenon in hematopoietic cells. Furthermore, we found that cell adhesion is inhibited by antioxidants such as (-)-epigallocatechin-3-gallate (EGCG), morin, and a-tocopherol. Our results suggest that albumin may inhibit basal cell adhesion of hematopoietic cells and that the oxidative balance in the plasma may be important for cell adhesion of hematopoietic cells in vivo.

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“…Thus under same (exercise, training) conditions with increased oxidative stress, supplementation of green tea polyphenols may attenuated ROS mediated tissue damage. In addition, EGCG inhibits proinflammatory cytokine and chemokine expression (Dona et al, 2003), decreases the expression of integrins and chemokines (monocyte chemoattractant protein-1) (Majkova, Smart, Toborek, Hennig, 2009), vascular cell adhesion proteins, (Ludwig et al, 2004), (Han et al, 2012), helps lower adhesion and migration of mast cells and reduces monocyte recruitment (Hofbauer et al, 1999). EGCG-mediated inhibition of chemokine Agata .…”

Section: Discussionmentioning

confidence: 99%

Effect of Long-Term Green Tea Extract Supplementation on Peripheral Blood Leukocytes in CrossFit-Trained and Untrained Men

Żak

Pokora

2017

Central European Journal of Sport Sciences and Medicine

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The aim of the study was to evaluate the effects of long-term green tea extract (GTE) supplementation on white blood cells (WBC) in CrossFit-trained and untrained men. Twenty-eight men were recruited for this study including CrossFit-trained (CF-TR) and untrained (CF-UNT) men. The study was carried out in two phases. Phase 1 involved non-supplemented untrained and trained men. Phase 2 involved the same groups but after 6-week supplementation with 2 GTE capsules once daily. Body weight, body composition and the number and percentage of peripheral blood leukocyte populations were assessed in each phase. The results revealed that the long-term green tea supplementation did not have a significant effect on body weight, body composition and the total white blood cell count of the study participants. However, the percentage of peripheral blood eosinophils increased while monocyte and neutrophil counts decreased. The changes in white blood cells were less pronounced in CrossFit-trained compared to control participants. Summing up, it should be noted that long-term GTE supplementation in CrossFit-trained men did not result in significant changes in body weight and composition and the total white blood cell count. However, the changes in leukocyte subsets were less pronounced in CrossFit-trained compared to untrained participants.

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The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells

Cited by 183 publications

References 33 publications

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Albumin and Antioxidants Inhibit Serum-deprivation-induced Cell Adhesion in Hematopoietic Cells

Effect of Long-Term Green Tea Extract Supplementation on Peripheral Blood Leukocytes in CrossFit-Trained and Untrained Men

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