The Taxonomic Significance of Fermentative Versus Oxidative Metabolism of Carbohydrates by Various Gram Negative Bacteria

Hugh, Rudolph; Leifson, Einar

doi:10.1128/jb.66.1.24-26.1953

Cited by 1,528 publications

(443 citation statements)

References 2 publications

(2 reference statements)

Supporting

Mentioning

424

Contrasting

Unclassified

Order By: Relevance

“…For identifying the isolates, they were subjected to phenotypic characterization such as Gram's staining, motility, production of catalase, oxidase, gelatinase, indole, methyl red test, Voges Proskauer reaction, citrate utilization, arginine dihydrolase, b-galactosidase (ONPG) and reduction in nitrate to nitrite (Holding & Collee, 1971;Stanier, Palleroni & Doudoroff, 1966;Stolp & Gadkari, 1981), aesculin hydrolysis (Gemmell & Hodgkiss, 1964), phenyl alanine deaminase (Cowan & Steel, 1974), H 2 S production (Cowan & Steel, 1965), lipase production (Tween-20, Tween-40, Tween-60, Tween-80) (Sierra, 1957), 3-ketogluconate (Haynes, 1951) Hugh and Leifson(1953) for the production of acid and gas.…”

Section: Phenotypic Characterization Of the Isolatesmentioning

confidence: 99%

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

et al. 2017

View full text Add to dashboard Cite

Nitrate reducing potency of 88 bacterial isolates segregated from marine and brackish water nitrifying bacterial consortia (NBC), generated for activation of nitrifying bioreactors, was confirmed by determining the nitrate reducing capability under aerobic conditions as maintained in nitrifying bioreactors. All the isolates had the potential to be used as bio‐augmentors for activating nitrate dissimilation in recirculating aquaculture system. The existence of nitrate reducers with nitrifiers in NBC and in the reactor configuration negates the requirement of integrating anoxic denitrifying system for effective removal of NO3−‐N. Phylogenetic analyses of representative isolates from each cluster of the dendrograms generated based on phenotypic characterization and amplified ribosomal DNA restriction analysis revealed profound diversity of nitrate reducing bacterial flora in the NBC. They were composed of Streptomyces enissocaesilis, Marinobacter sp., Pseudomonas sp., Microbacterium oxydans, Pelagibacterium halotolerans and Alcanivorax dieselolei from marine NBC and Streptomyces tendae, Nesterenkonia sp., Bacillus cereus, Microbacterium oxydans and Brevibacterium sp. from brackish water NBC. The diversity indices of the consortia were calculated using Mega 5.0, primer 7 and VITCOMIC softwares. Marine NBC exhibited higher Shannon wiener diversity and mean population diversity than brackish water NBC. The study delineated higher species richness and diversity in marine NBC than in its brackish water counterpart, a possible reflection of the higher biodiversity of marine systems, and hence, the former is more promising to be used as start‐up cultures for the activation of nitrifying bioreactors after appropriate acclimatization to the desired salinity.

show abstract

Section: Phenotypic Characterization Of the Isolatesmentioning

confidence: 99%

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In these and subsequent tests inoculations were made from nutnent broth cultures grown for 24 h at 25" C. Unless stated otherwise, tests were performed using the multipoint inoculation technique and plates incubated at 25" C for 3 days. Hugh and Leifson's (1953) medium was dispensed to a depth of about P were taken Fi rom half-way down the A, horizon and from a corres o n i n g tubes was added to one of two flasks containing $00 ml of steri P e one-…”

Section: Introductionmentioning

confidence: 99%

Properties and Composition of the Bacterial Flora of a Pine Forest Soil

Goodfellow

1968

Journal of Soil Science

View full text Add to dashboard Cite

A study was made of the qualitative and quantitative distribution of aerobic heterotrophic bacteria in the two mineral horizons of a developing podsol soil under pimcs. There was no significant difference in the total numbers or in the kinds of bacteria between the two horizons. In the acidic A, horizon (pH, 3.6) Bacillus spp. comprised 58 per cent of the population, the remainder being composed of Gram-negative rods (15 per cent), pleomorphic rods (14 per cent), Grampositive cocci (10 per cent) and streptomycetes (3 per cent). The corresponding figures for the C horizon (pH, 8.6) were Bacillus (23 per cent), Gram-negative rods (37 per cent), pleomorphic rods (10 per cent), Gram-positive cocci (17 per cent) and streptomycetes (13 per cent). These differences in distribution could not be related to any one environmental factor. The data from this investigation lend support to the view that different soil types can be distinguished by their bacterial floras.Bacillus subtilis, B. cereus and the Gram-positive cocci were biochemically and fennentatively active and many of the streptomycetes were able to degrade complex organic molecules. The Gram-negative and pleomorphic forms were apparently metabolically inactive.

show abstract

“…sensitivity to 3% KOH (Suslow, Schroth, & Isaka, 1982), oxidative/ fermentative metabolism (Hugh & Leifson, 1953), indole production (Fahy & Hayward, 1983) and oxidase reaction (Kovacs, 1956).…”

Section: Phenotypic Testsmentioning

confidence: 99%

Brenneria spp. and Rahnella victoriana associated with acute oak decline symptoms on oak and hornbeam in Iran

et al. 2019

View full text Add to dashboard Cite

Decline diseases of forest trees are complex syndromes not attributable to single causal factors. In Iran, symptoms of decline disease have been observed in a number of native forest species including Quercus castaneifolia (chestnut‐leaved oak), Q. brantii (Persian oak) and Carpinus betulus (hornbeam). The symptoms are prevalent in the northern forests and the Zagros mountain forests. There are parallels between the disease in Iran and acute oak decline (AOD) reported in the UK, specifically the presence of weeping cankers, which have been attributed to a polybacterial complex wherein Brenneria goodwinii is considered a key necrogen. Based on the AOD symptomatology, and as a first step towards discovering potential causal agents of the stem weeping symptoms of affected trees in Iran, necrotic tissues were tested primarily for the presence of B. goodwinii. Symptomatic Q. castaneifolia and C. betulus from the Mazandaran Province and symptomatic Q. brantii from Kohgiluyeh and Boyerahmad Province were sampled. Isolation and culture on a selective medium yielded uniform bacterial colonies. Isolates were characterized using phenotypic and genotypic (DNA sequencing) tests. The isolates were phenotypically identical to members of Pectobacteriaceae and Yersiniaceae, specifically Brenneria and Rahnella spp. The nucleotide sequences of the 16S rRNA and housekeeping genes gyrB, infB and atpD (MLSA) amplified via PCR demonstrated that the isolates from the trees in Iran were indeed B. goodwinii, B. roseae subsp. roseae, Rahnella victoriana and an unknown species of Brenneria. Most bacteria isolated from non‐symptomatic trees were Gram‐positive, and Pseudomonas spp. were dominant, but bacterial species isolated from the diseased trees were not detected in healthy trees. Hypersensitivity response tests were positive, but inoculation on saplings was more variable with internal necrosis developing only once in the test period. Therefore, further testing is required. This is the first report of the incidence of B. goodwinii, B. roseae subsp. roseae, R. victoriana and Brenneria sp. associated with acute oak decline‐like symptoms on Q. castaneifolia, Q. brantii and C. betulus across the western forests of Iran and in the world.

show abstract

The Taxonomic Significance of Fermentative Versus Oxidative Metabolism of Carbohydrates by Various Gram Negative Bacteria

Cited by 1,528 publications

References 2 publications

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems

Properties and Composition of the Bacterial Flora of a Pine Forest Soil

Brenneria spp. and Rahnella victoriana associated with acute oak decline symptoms on oak and hornbeam in Iran

Contact Info

Product

Resources

About