“…For consistency, Culex pipiens was used for studies reporting Culex pipiens complex and Culex pipiens sensu lato [ 37 , 38 ]. Likewise, Ochlerotatus caspius and Ochlerotatus scapularis were denoted as Aedes caspius and Aedes scapularis , respectively, as both genus names were used in the literature [ 39 ]. Virus detection methods were classified into four categories: 1) sequencing, for samples that were directly analyzed by next-generation sequencing, 2) PCR, for samples in which viruses of a particular virus taxon or species were detected by PCR, 3) culture-sequencing, when mosquito homogenate was cultured on mosquito cell lines, after which viruses were detected by next-generation sequencing, and 4) culture-PCR, when mosquito homogenate was cultured on cell lines and virus was detected by PCR using virus taxon or species specific PCR primers.…”