The taste system of small fish species

Okada, Shinji

doi:10.1080/09168451.2015.1023251

Cited by 18 publications

(10 citation statements)

References 25 publications

(26 reference statements)

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“…It will be interesting in future studies to confirm whether all of the pkd expression in zebrafish pharyngeal regions corresponds to taste receptors and which pkd genes are co-expressed in particular subsets of these cells. If, indeed, pkd gene expression demarcates different types of receptor cells, these additional pkd expression domains in zebrafish might be consistent with suggestions that teleosts have relatively large numbers of different taste receptors (Okada, 2015 ).…”

Section: Discussionsupporting

confidence: 81%

Identification and Expression Analysis of the Complete Family of Zebrafish pkd Genes

England

Campbell

Banerjee

et al. 2017

Front. Cell Dev. Biol.

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Polycystic kidney disease (PKD) proteins are trans-membrane proteins that have crucial roles in many aspects of vertebrate development and physiology, including the development of many organs as well as left–right patterning and taste. They can be divided into structurally-distinct PKD1-like and PKD2-like proteins and usually one PKD1-like protein forms a heteromeric polycystin complex with a PKD2-like protein. For example, PKD1 forms a complex with PKD2 and mutations in either of these proteins cause Autosomal Dominant Polycystic Kidney Disease (ADPKD), which is the most frequent potentially-lethal single-gene disorder in humans. Here, we identify the complete family of pkd genes in zebrafish and other teleosts. We describe the genomic locations and sequences of all seven genes: pkd1, pkd1b, pkd1l1, pkd1l2a, pkd1l2b, pkd2, and pkd2l1. pkd1l2a/pkd1l2b are likely to be ohnologs of pkd1l2, preserved from the whole genome duplication that occurred at the base of the teleosts. However, in contrast to mammals and cartilaginous and holostei fish, teleosts lack pkd2l2, and pkdrej genes, suggesting that these have been lost in the teleost lineage. In addition, teleost, and holostei fish have only a partial pkd1l3 sequence, suggesting that this gene may be in the process of being lost in the ray-finned fish lineage. We also provide the first comprehensive description of the expression of zebrafish pkd genes during development. In most structures we detect expression of one pkd1-like gene and one pkd2-like gene, consistent with these genes encoding a heteromeric protein complex. For example, we found that pkd2 and pkd1l1 are expressed in Kupffer's vesicle and pkd1 and pkd2 are expressed in the developing pronephros. In the spinal cord, we show that pkd1l2a and pkd2l1 are co-expressed in KA cells. We also identify potential co-expression of pkd1b and pkd2 in the floor-plate. Interestingly, and in contrast to mouse, we observe expression of all seven pkd genes in regions that may correspond to taste receptors. Taken together, these results provide a crucial catalog of pkd genes in an important model system for elucidating cell and developmental processes and modeling human diseases and the most comprehensive analysis of embryonic pkd gene expression in any vertebrate.

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Section: Discussionsupporting

confidence: 81%

Identification and Expression Analysis of the Complete Family of Zebrafish pkd Genes

England

Campbell

Banerjee

et al. 2017

Front. Cell Dev. Biol.

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“…Consistent with the view that T1R proteins function as heterodimeric complexes, co-expression of T1R3 with either T1R1 or T1R2 has been largely reported in taste receptor cells of both mammals and fish [ 17 , 30 , 31 ]. Additionally, in vitro functional studies have been performed in several species, where it is generally assumed that the extent of T1R activation following L-AA and sugar stimuli is positively correlated to taste sensations [ 20 , 32 , 33 ].…”

Section: Resultsmentioning

confidence: 60%

Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)

Angotzi

Puchol

Cerdá‐Reverter

et al. 2020

IJMS

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A plethora of molecular and functional studies in tetrapods has led to the discovery of multiple taste 1 receptor (T1R) genes encoding G-protein coupled receptors (GPCRs) responsible for sweet (T1R2 + T1R3) and umami (T1R1 + T1R3) taste. In fish, the T1R gene family repertoires greatly expanded because of several T1R2 gene duplications, and recent studies have shown T1R2 functional divergence from canonical mammalian sweet taste perceptions, putatively as an adaptive mechanism to develop distinct feeding strategies in highly diverse aquatic habitats. We addressed this question in the carnivore fish gilthead seabream (Sparus aurata), a model species of aquaculture interest, and found that the saT1R gene repertoire consists of eight members including saT1R1, saT1R3 and six saT1R2a-f gene duplicates, adding further evidence to the evolutionary complexity of fishT1Rs families. To analyze saT1R taste functions, we first developed a stable gene reporter system based on Ca2+-dependent calcineurin/NFAT signaling to examine specifically in vitro the responses of a subset of saT1R heterodimers to L-amino acids (L-AAs) and sweet ligands. We show that although differentially tuned in sensitivity and magnitude of responses, saT1R1/R3, saT1R2a/R3 and saT1R2b/R3 may equally serve to transduce amino acid taste sensations. Furthermore, we present preliminary information on the potential involvement of the Gi protein alpha subunits saGαi1 and saGαi2 in taste signal transduction.

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“…Sensory system, as a process of which teleost fishes respond to diverse stimuli in aquatic milieu, largely is classified into mechano-and chemo-receptions by their differences in a route of innervation, a passage of signal transduction cascade, and a type of stimulation (Hara 1986;Okada 2015). Among them, the olfaction is one of chemoreception that senses only a mixed aquatic compound such as hazard molecules, hormones, bile acid and amino acids dissolved in animal's habitat, through the dendrites of olfactory receptor neurons (ORNs) to contact the surface of the epithelia (Hara 1994;Hanson et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Anatomy, ultrastructure and histology of the olfactory organ of the largemouth bass Micropterus salmoides, Centrarchidae

2019

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The detailed anatomy, ultrastructure and histology of the olfactory organ of Micropterus salmoides were investigated by a stereo microscope, a light microscope, and a scanning electron microscope. Its external structure shows a tube-like anterior nostril to stick out and a posterior nostril flat to the skin surface. Meanwhile, its internal structure, the olfactory chamber, contains a fan-shaped rosette structure with 9 to 11 lamellae in adult fish over 35 cm in standard length (SL) and two accessory nasal sacs (ethmoidal and lacrimal sacs) were found. Interestingly, the rosette in young fish under 15 cm in SL was a longitudinal structure in parallel with each of 4-5 lamellae. Histologically, the sensory epithelium (SE) on the olfactory chamber consists of 5 types of cells: olfactory receptor neurons, supporting cells, basal cells, lymphatic cells and mucous cells. In contrast, the non-sensory epithelium (NSE) has stratified epithelial cells, lymphatic cells and mucous cells. The mucous cells of the SE are abundant and distributed densely in one row on the outermost superficial surface, but the one of the NSE are less than the SE. From these results, the olfactory characters of M. salmoides may be related with its ecological habit spending in the middle layer of stagnant water contaminated, more or less.

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The taste system of small fish species

Cited by 18 publications

References 25 publications

Identification and Expression Analysis of the Complete Family of Zebrafish pkd Genes

Identification and Expression Analysis of the Complete Family of Zebrafish pkd Genes

Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)

Anatomy, ultrastructure and histology of the olfactory organ of the largemouth bass Micropterus salmoides, Centrarchidae

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