Introduction: Anatomical knowledge and understanding are key components of physiotherapy education and practice. Traditionally, anatomy has been taught as a foundation stream within the first year(s) of physiotherapy education. This curricular model is based on the assumption that further learning in subsequent years builds upon the knowledge gained in the early stages of the program. However, the retention rate in all basic sciences has often been called into question. In anatomy, several studies suggest that anatomy knowledge endures considerable attrition, highlighting the need for the evaluation of retention rates. This paper aimed at making a preliminary assessment of the knowledge and retention of anatomy among physiotherapy students.