The Tangential Nucleus Controls a Gravito-inertial Vestibulo-ocular Reflex

Bianco, Isaac H.; Ma, Leung-Hang; Schoppik, David; Robson, Drew N.; Orger, Michael B.; Beck, James C.; Li, Jennifer Mengbo; Schier, Alexander F.; Engert, Florian; Baker, R.

doi:10.1016/j.cub.2012.05.026

Cited by 113 publications

(191 citation statements)

References 47 publications

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“…Vertebrate eye movements occur in three dimensions (Fig 1A-C), which enables tracking of visual targets and stabilization of gaze with respect to world- or self-motion 11,12 . The connectivity and physiological function of individual nuclei comprising the hindbrain circuits generating these oculomotor behaviors have been extensively characterized 13-15 .…”

Section: Introductionmentioning

confidence: 99%

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Development of oculomotor circuitry independent of hox3 genes

Grove

Baker

2014

Nat Commun

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Hox genes have been shown to be essential in vertebrate neural circuit formation and their depletion has resulted in homeotic transformations with neuron loss and miswiring. Here we quantifiy four eye movements in the zebrafish mutant valentino and hox3 knockdowns and find that contrary to the classical model, oculomotor circuits in hindbrain rhombomeres 5-6 develop and function independently from hox3 genes. All subgroups of oculomotor neurons are present as well as their input and output connections. Ectopic connections are also established, targeting two specific subsets of horizontal neurons and the resultant novel eye movements coexist with baseline behaviors. We conclude that the high expression of hox3 genes in rhombomeres 5-6 serves to prevent an aberrant neuronal identity and behaviors, but does not appear to be necessary for a comprehensive assembly of functional oculomotor circuits.

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Section: Introductionmentioning

confidence: 99%

“…Horizontal and torsional/vertical eye movements emerge stereotypically in larval zebrafish (Fig. 1A-C) 11,12 . Quantitative measurements of these behaviors allow simultaneous assessment of multiple neuronal subgroups and connections in vivo and enable a comprehensive characterization of circuit development.…”

Section: Introductionmentioning

confidence: 99%

Development of oculomotor circuitry independent of hox3 genes

Grove

Baker

2014

Nat Commun

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“…Moreover, our observations of the eyes represent the vestibulo-ocular reflex (VOR), a threeneuron circuit that has been previously described in various vertebrates, including larval zebrafish [122]. In this study, changing a larva's pitch resulted in compensatory eye movements, while we see rolling eye movements in response to a perceived roll of the larva's body.…”

Section: Tail and Eyes Movements In The Literaturesupporting

confidence: 62%

“…Previous studies on acceleration sensing involve the development of a complex translational robot [121] or rotating platform [29,122], but such techniques are disruptive as the whole fish body is accelerated. This also implies that the behavioural response can be difficult to extract, as the fish body encounters changing forces due to changing water flow or gravity, which will affect the behavioural response too.…”

Section: Necessity Of a Non-invasive Systemmentioning

confidence: 99%

“…Also, to visualize the larva's behavioural responses, we need to image its whole body, because we are interested in the tail and eyes, since these are likely to be moved to compensate for perceived motion [29,122,136]. The need to apply two traps and image the body simultaneously led us to build a new set-up different from the set-ups presented previously, but with some similarities to the optogenetics system presented in section 3.1.2.…”

Section: Experimental Set-upmentioning

confidence: 99%

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Imaging, manipulation and optogenetics in zebrafish

Favre-bulle¹

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This thesis investigates the advantages and limits of using laser light for illumination and micromanipulation of the nervous system deep in-vivo in zebrafish. Further interest in the study neuronal pathways and functions in zebrafish led to employ laser micro-manipulation for the stimulation of the systems of interest and the development of optogenetics system and light sheet microscopy for analyses of the processes. First I present the investigation of the interaction of light and brain tissue as we aim to quantify light scattered in zebrafish brain tissue with depth. This is of great importance for optogenetics as it uses light to control (turn on or off) neurons and the unwanted/scattered light out of the aimed region could significantly affect results of experiments. The measurements and model developed to predict light propagation through brain tissue and how much a focussed beam broadens and alters with depth are presented in details. Results show that illumination of individual neurons is possible at depth in the zebrafish brain, despite scattering that results from shallower neural tissue. This means that the approach presented allows for optogenetics manipulation of single neurons to be performed without optical correction for scattering. Secondly, I construct an optogenetics system where we employ advanced optics and novel algorithm to deliver versatile two dimensional illumination that can be used at any depth in the fish. I present an example of a problem that I have solved, and resulting optogenetics results that were obtained, using this new technology. In chapter 5, I was responsible for the improvements implemented on the optogenetics system and the different tests to show its performances. Lucy Heap has performed multiple experiments on that system and I present one example she performed and the results she got.In chapter 7, I performed the manipulation of free otolith in water. I with Alex Stilgoe performed scanning experiments of otolith on an optical trapping system that Alex Stilgoe built.We both performed the analysis of the experimental results. Alex Stilgoe performed the force modelisation with Ray optics methods and we compared it with experimental results. Statement of parts of the thesis submitted to qualify for the award of another degreeNone. I have special thanks to Shu, Swaantje and Lucy for our philosophical discussions, the sharing of our experiences in the good and bad moments of our PhDs and their support.Finally I have some friends that I would like to thank for their great support without necessarily realising it: Mari-wenn, Estelle and Arnaud.8 ABSTRACT

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Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Greaney

Privorotskiy

D’Elia

et al. 2016

J of Comparative Neurology

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Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotemporal trajectory of maturation to circuit‐level functional organization. This study examined the development of two extraocular motor nuclei (nIII and nIV), structures in which a motoneuron's identity, or choice of muscle partner, defines its behavioral role. We used retro‐orbital dye fills, in combination with fluorescent markers for motoneuron location and birthdate, to probe spatial and temporal organization of the oculomotor (nIII) and trochlear (nIV) nuclei in the larval zebrafish. We describe a dorsoventral organization of the four nIII motoneuron pools, in which inferior and medial rectus motoneurons occupy dorsal nIII, while inferior oblique and superior rectus motoneurons occupy distinct divisions of ventral nIII. Dorsal nIII motoneurons are, moreover, born before motoneurons of ventral nIII and nIV. The order of neurogenesis can therefore account for the dorsoventral organization of nIII and may play a primary role in determining motoneuron identity. We propose that the temporal development of extraocular motoneurons plays a key role in assembling a functional oculomotor circuit. J. Comp. Neurol. 525:65–78, 2017. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

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The Tangential Nucleus Controls a Gravito-inertial Vestibulo-ocular Reflex

Cited by 113 publications

References 47 publications

Development of oculomotor circuitry independent of hox3 genes

Development of oculomotor circuitry independent of hox3 genes

Imaging, manipulation and optogenetics in zebrafish

Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

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