The tail suspension test: Ethical considerations

Thierry, Bernard; Stéru, L.; Simon, Pierre; Porsolt, Roger D.

doi:10.1007/bf00181261

Cited by 110 publications

(56 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Behavioral despair illustrates that the animal has lost the motivation to perform escape-oriented behavior (Porsolt et al 1977). TST is predicted to give less stress in animals with more pharmacological sensitivity when compared to FST (Thierry et al 1986;Zeni et al 2011). In this test, animals show vigorous activity followed by progressive periods of immobility, aimed at rescuing themselves from the unpleasant situation termed helplessness.…”

Section: Discussionmentioning

confidence: 99%

Antidepressant and anxiolytic effects of Garcinia indica fruit rind via monoaminergic pathway

2017

View full text Add to dashboard Cite

Depression and anxiety are the most crippling neuropsychiatric disorders of this modern era. These mostly occur as anxiety followed by depression or in mixed state. Therefore, there is an urgent need of a safe and effective treatment, which proves its worth in this ailment. What else than a conventional food would be a better choice for a convenient therapy. Therefore, Garcinia indica, commonly known as Kokam, fruit rind has been used in the present study to investigate its antidepressant and anti-anxiety potential using forced swim test, tail suspension test, and reserpine-induced hypothermia; and elevated plus maze, hole-board test, and light dark model, respectively. Garcinia indica fruit rind given to mice with food for consecutive 14 days at 0.5, 1, and 2% w/w significantly (p \ 0.05) reduced despair behavior in forced swim test, immobility duration in tail suspension test, and also switched the hypothermia (reserpine induced) to normal temperature significantly (p \ 0.05). Garcinia indica significantly (p \ 0.05) raised the time elapsed and count of entries in open arms of elevated plus maze, enhanced incidence of head dipping in holes of hole board along with duration of expending time in lit compartment of light dark model, exhibiting its anti-anxiety effect. Garcinia indica significantly reduced monoamine oxidase and malondialdehyde levels providing support to neuroprotective potential of fruit rind. The mechanistic study showed the participation of G. indica at a 1 -adrenoceptor and D 2 -dopamine receptor, by attenuating prazosin and sulpirideinduced increase in immobility duration. Garcinia indica fruit rind showed a significant antidepressant and anxiolytic effect while no effect on locomotor activity, i.e., no psycho-stimulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Antidepressant and anxiolytic effects of Garcinia indica fruit rind via monoaminergic pathway

2017

View full text Add to dashboard Cite

show abstract

“…The total duration of immobility induced by tail suspension was measured according to the method described by steru [15] and Thierry [16]. Mice were suspended on the edge of a table 50 cm above the floor by the adhesive tape placed approximately 1 cm from the tip of the tail.…”

Section: Tstmentioning

confidence: 99%

Phytochemical and Pharmacological Evaluation of Callistemon Citrinus for Antidepressant Activity in Albino Mice

Pendyala¹,

Thaakur²

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The present study was carried out to find out the antidepressant activity of different fractions of stems and leaves of Callistemon citrinus (CC). The efficacy of the fractions was compared with the standard reference drug imipramine. Methods:All the studies were conducted according to the ethical guidelines of CPCSEA. Healthy adult albino mice weighing 20-30 g were used as experimental animals. The leaves of CC were extracted with alcohol and fractionated with chloroform and petroleum ether. All the fractions were subjected for preliminary phytochemical screening, using various qualitative tests. In the present investigation, tail suspension test (TST) and forced swim test (FST) are selected as animal models for evaluation of antidepressant activity in albino mice. Results:The preliminary phytochemical screening of CC has revealed the presence of carbohydrates, tannins, and flavonoids in a hydroalcoholic fraction. Chloroform fraction showed positive results toward flavonoids, alkaloids, tannins, glycosides, and steroids. Alcoholic and chloroform extract (100 and 200 mg/kg p.o.) of CC administered orally for 14 successive days had decreased the immobility periods significantly in a dose-dependent manner in both TST and FST, showing significant antidepressant-like activity. The activities of the extracts were found to be comparable to imipramine in both FST and TST.Conclusions: Although a number of synthetic drugs are being used as a standard treatment for clinically depressed patient, they have adverse effects that can compromise the therapeutic treatment. In the traditional systems of medicine, many plants and formulations have been used to treat depression for thousands of years. The results of the study indicate that CC can be used as an antidepressant herb.

show abstract

“…16 In this study, rats are suspended by their tail for defined period of time (usually 6min.) and duration of their immobility is assessed.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of L. siceraria against depression using behavioural despair animal models

Kota

Sharma

Ragavendra³

et al. 2018

Int J Basic Clin Pharmacol

View full text Add to dashboard Cite

Background: In recent years, the search for novel pharmacotherapy from medicinal plants for psychiatric illness was significantly progressed. The present study was performed to evaluate the antidepressant activity of ethanolic extract of Lagenaria siceraria in animal models.Methods: The antidepressant activity of ethanolic extract of the fruit of L. siceraria in rats was assessed using forced swim test and tail suspension test. Imipramine at 15 mg/kg was used as standard antidepressant drug.Results: The ethanolic extract of L. siceraria fruit (EELS) was significantly and dose-dependently reduced the duration of immobility after repeated treatment for 7 days in Forced swim test and Tail suspension Test. But combination of L. siceraria (200mg/kg) with Imipramine gave a highly significant result (p<0.001) in reduction of immobility duration and the effect of high dose (400mg/kg) with imipramine (15mg/kg) did not decrease the duration of immobility period in both animal models at end of the study. In this work the dose of 400mg/kg afforded more protection than the imipramine.Conclusions: The results obtained from this study was indicate that the antidepressant activity of L. siseraria.

show abstract

The tail suspension test: Ethical considerations

Cited by 110 publications

References 3 publications

Antidepressant and anxiolytic effects of Garcinia indica fruit rind via monoaminergic pathway

Antidepressant and anxiolytic effects of Garcinia indica fruit rind via monoaminergic pathway

Phytochemical and Pharmacological Evaluation of Callistemon Citrinus for Antidepressant Activity in Albino Mice

The protective effect of L. siceraria against depression using behavioural despair animal models

Contact Info

Product

Resources

About