The TAFII250 Subunit of TFIID Has Histone Acetyltransferase Activity

Abstract

The transcription initiation factor TFIID is a multimeric protein complex composed of TATA box-binding protein (TBP) and many TBP-associated factors (TAF(II)s). TAF(II)s are important cofactors that mediate activated transcription by providing interaction sites for distinct activators. Here, we present evidence that human TAF(II)250 and its homologs in Drosophila and yeast have histone acetyltransferase (HAT) activity in vitro. HAT activity maps to the central, most conserved portion of dTAF(II)230 and yTAF(II)130. The HAT activity of dTAF(II)230 resembles that of yeast and human GCN5 in that it is specific for histones H3 and H4 in vitro. Our findings suggest that targeted histone acetylation at specific promoters by TAF(II)250 may be involved in mechanisms by which TFIID gains access to transcriptionally repressed chromatin.

The cloning, mapping and expression of a novel gene, BRL, related to the AF10 leukaemia gene

“…The bromodomain appears to be highly conserved through evolution and is associated with many transcription factors having a role in histone acetylation including yeast and human GCN5 Candau et al, 1996;Georgakopoulos and Thireos, 1992;Wang et al, 1997), p300/CBP (Arany et al, 1994;Arias et al, 1994;Bannister and Kouzarides, 1996;Chrivia et al, 1993;Kwok et al, 1994;, pCAF and hTAF II 250 (Mizzen et al, 1996). It has, however, been shown by several groups that the bromodomain is dispensable for HAT activity Mizzen, 1996 #77;Ogryzko, 1996 #76).…”

The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment of N-CoR

“…Deacetylation of histone N-terminal tails can reverse this process and hypoacetylated regions of chromatin are transcriptionally less accessible. E1A interacts with various chromatin modifying proteins, (reviewed by Mymryk and Smith, 1997), some of which display histone acetyltransferase (HAT) activity, such as the transcriptional coactivators CBP/p300 (Ogryzko et al, 1996) and TAF II -250 (Geisberg et al, 1995;Mizzen et al, 1996). Other E1A binding proteins are directly associated with histone deacetylase proteins (HDAc's), like the Rb family of transcriptional repressors (Luo et al, 1998;Brehm et al, 1998;Magnaghi et al, 1998;Ferreira et al, 1998) and the transcriptional regulators YY-1 (Lee et al, 1995;Lewis et al, 1995;Yang et al, 1997) and CtBP (Schaeper et al, 1995;Sundqvist et al, 1998).…”

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAFII250 mutation

“…tsBN462 is in same complementation group as ts13, an independently isolated BHK cell mutant and both have an identical Gly690Asp point mutation in CCG1/TAF II 250 (Hayashida et al, 1994). CCG1/TAF II 250 is a key component of the TFIID complex, which is required for RNA polymerase II dependent transcription (Hisatake et al, 1993;Ruppert et al, 1993); CCG1/TAF II 250 has both protein kinase and acetyltransferase activities (Dikstein et al, 1996;Mizzen et al, 1996). At the restrictive temperature of 39.58C, tsBN462 cells arrest in G1 (Sekiguchi et al, 1987) and subsequently undergo apoptosis (Sekiguchi et al, 1995).…”