The tachykinin NH2-senktide, a selective neurokinin B receptor agonist, is a very potent inhibitor of salt appetite in the rat

Massi, Maurizio; Polidori, Carlo; Gentili, Luigi; Perfumi, Marina; de, G.; Maggi, Carlo Alberto

doi:10.1016/0304-3940(88)90614-3

Cited by 46 publications

(13 citation statements)

References 18 publications

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“…The present study revealed intense expression of NKR-HS and NKR-LI in many regions of the hypothalamus, nucleus of the solitary tract, and dorsal motor nucleus of the vagus nerve, suggesting the involvement of NKR in autonomic functions. It has been reported that intracerebroventricular injection of NHz-senktide, a synthetic peptide highly selective for NKR (Wormser et al, 1986;Laufer et al, 19881, potentially inhibits salt appetite (Massi et al, 1988) and that microinjection of senktide into the nucleus of the solitary tract causes long-lasting hypertension and tachycardia (Nagashima et al, 1989). It is also likely that intracerebroventricular injection of NK increases the blood pressure mainly via release of vasopressin from the hypothalamus through NKR (Polidori et al, 1989;Takano et al, 1990).…”

Section: Discussionmentioning

confidence: 97%

Localization of the neuromedin K receptor (NK3) in the central nervous system of the rat

et al. 1996

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The distribution of the neuromedin K receptor (NK3; NKR) in the central nervous system was investigated in the adult rat by using in situ hybridization and immunohistochemical techniques. The rabbit anti-NKR antibody was raised against a bacterial fusion protein containing a C-terminal portion of NKR and affinity purified with a Sepharose 4B column conjugated to the fusion protein. Immunoblot analysis was performed to test the reactivity and specificity of the antibody. Crude membrane was prepared from cDNA-transfected Chinese hamster ovary (CHO) cells expressing each of the rat NKR, substance P receptor (NK1; SPR), and substance K receptor (NK2; SKR) and from the hypothalamus, cerebral cortex, and cerebellum. Immunoreactive bands were observed specifically in the NKR-CHO cells, hypothalamus, and cerebral cortex but not in the SPR- or SKR-CHO cells, nor in the cerebellum. Molecular weights of the immunoreactive bands ranged from 73 to 89 kDa and from 59 to 83 kDa in the NKR-CHO cells and tissues, respectively. The distribution of NKR-like immunoreactivity coincided with that of NKR mRNA. The expression of NKR was indicated on neuronal cell bodies and dendrites. NKR was found to be expressed intensely or moderately in neurons in the glomerular and granule cell layers of the main olfactory bulb; glomerular and mitral cell layers of the accessory olfactory bulb; layers IV and V of the cerebral neocortex; medial septal nucleus; nucleus of the diagonal band; bed nucleus of the stria terminalis; globus pallidus; ventral pallidum; paraventricular nucleus; supraoptic nucleus; zona incerta; dorsal, lateral, and posterior hypothalamic areas; amygdaloid nuclei; medial habenular nucleus; ventral tegmental area; midbrain periaqueductal gray; interpeduncular nuclei; substantia nigra pars compacta; linear, median, dorsal, and pontine raphe nuclei; posteromedial tegmental nucleus; sphenoid nucleus; nucleus of the solitary tract; intermediate and rostroventrolateral reticular nuclei; and lamina II of the caudal spinal trigeminal nucleus and spinal dorsal horn. These findings are discussed in relation to the physiological functions associated with neuromedin K.

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Section: Discussionmentioning

confidence: 97%

Localization of the neuromedin K receptor (NK3) in the central nervous system of the rat

et al. 1996

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“…The tachykinin family to which Lk belongs has been implicated in the regulation of satiety and sleep, however any physiological connections between these behavioral outputs has not been explored (Massi et al, 1988; Zielinski et al, 2015). The orexin system is regulated by a diversity of signaling molecules (Scammell and Winrow, 2011) and has already shown promise as a target for sleep therapeutics.…”

Section: Discussionmentioning

confidence: 99%

Postprandial sleep mechanics in Drosophila

Murphy

Deshpande

Yurgel

et al. 2016

eLife

119

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Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged from slightly arousing to strongly sleep inducing. Postprandial sleep was positively correlated with ingested volume, protein, and salt—but not sucrose—revealing meal property-specific regulation. Silencing of leucokinin receptor (Lkr) neurons specifically reduced sleep induced by protein consumption. Thermogenetic stimulation of leucokinin (Lk) neurons decreased whereas Lk downregulation by RNAi increased postprandial sleep, suggestive of an inhibitory connection in the Lk-Lkr circuit. We further identified a subset of non-leucokininergic cells proximal to Lkr neurons that rhythmically increased postprandial sleep when silenced, suggesting that these cells are cyclically gated inhibitory inputs to Lkr neurons. Together, these findings reveal the dynamic nature of postprandial sleep.DOI: http://dx.doi.org/10.7554/eLife.19334.001

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“…Activation of NK 3 receptors affects neural transmission in several sensory systems, cardiovascular function (e.g., blood pressure and heart rate), pituitary secretion, and fluid and electrolyte balance (Papir-Kircheli et al, 1987;Massi et al, 1988bMassi et al, , 1991aNagashima et al, 1989;Polidori et al, 1989;Takano et al, 1993;Smith and Flynn, 1994;Couture et al, 1995;Eguchi et al, 1996). Given the importance of NK 3 receptors and their ligand in many regulatory functions, it is not surprising that investigators have sought to identify particular sites that mediate the actions of NK 3 agonists.…”

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confidence: 98%

Distribution of fos-like immunoreactivity within the rat brain following intraventricular injection of the selective NK3 receptor agonist senktide

Smith

Flynn

2000

J. Comp. Neurol.

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Neurokinin B (NKB) is one member of an evolutionarily conserved family of neuropeptides, the tachykinins. Preferential binding of NKB to endogenous NK(3) receptors affects a variety of biological and physiological processes, including endocrine secretions, sensory transmission, and fluid and electrolyte homeostasis. In light of its widespread biological actions, immunohistochemical detection of the c-Fos protein product was used to study the distribution of neuronal activation in the rat brain caused by intraventricular (icv) injections of the selective NK(3) receptor agonist (succinyl-[Asp(6), N-Me-Phe(8)] substance P [6-11]), senktide. Quantitative analysis revealed that treatment with isotonic saline or 200 ng senktide resulted in the differential expression of Fos-like immunoreactivity (FLI) throughout the brain. Senktide induced the highest number of FLI neurons in the lateral septum, bed nucleus of the stria terminalis, amygdala, paraventricular nucleus of the hypothalamus, median preoptic nucleus, organum vasculosum of the lamina terminalis, supraoptic nucleus, periaqueductal gray, and medial nucleus of the solitary tract compared to isotonic saline controls. Additional regions that contained elevated FLI following icv injection of senktide, relative to saline injection, included the cerebral cortex, lateral hypothalamic nucleus, suprachiasmatic nucleus, ventral tegmental area, substantia nigra, inferior colliculus, locus coeruleus, zona incerta, and arcuate nucleus. Our data indicate that activation of NK(3) receptors induces the expression of FLI within circumscribed regions of the rat brain. This pattern of neuronal activation overlaps with nuclei known to regulate homeostatic processes, such as endocrine secretion, cardiovascular function, salt intake, and nociception.

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The tachykinin NH2-senktide, a selective neurokinin B receptor agonist, is a very potent inhibitor of salt appetite in the rat

Cited by 46 publications

References 18 publications

Localization of the neuromedin K receptor (NK3) in the central nervous system of the rat

Localization of the neuromedin K receptor (NK3) in the central nervous system of the rat

Postprandial sleep mechanics in Drosophila

Distribution of fos-like immunoreactivity within the rat brain following intraventricular injection of the selective NK3 receptor agonist senktide

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