The T2 Mycobacterium tuberculosis Genotype, Predominant in Kampala, Uganda, Shows Negative Correlation with Antituberculosis Drug Resistance

Abstract

e Surveillance of the circulating Mycobacterium tuberculosis complex (MTC) strains in a given locality is important for understanding tuberculosis (TB) epidemiology. We performed molecular epidemiological studies on sputum smear-positive isolates that were collected for anti-TB drug resistance surveillance to establish the variability of MTC lineages with anti-TB drug resistance and HIV infection. Spoligotyping was performed to determine MTC phylogenetic lineages. We compared patients' MTC lineages with drug susceptibility testing (DST) patterns and HIV serostatus. , 5.0; 95% CI, 2.0 to 11.9; P < 0.001) and CAS (OR adj , 2.9; 95% CI, 1.4.0 to 6.3; P ‫؍‬ 0.006) families were more likely to show any resistance than was T2. All other MTC lineages combined were more likely to be resistant to any of the anti-TB drugs than were the T2 strains (OR adj , 1.7; 95% CI, 1.0 to 2.9; P ‫؍‬ 0.040). There were no significant associations between multidrug resistance and MTC lineages, but numbers of multidrug-resistant TB strains were small. No association was established between MTC lineages and HIV status. In conclusion, the T2 MTC lineage negatively correlates with anti-TB drug resistance, which might partly explain the reported low levels of anti-TB drug resistance in Kampala, Uganda. Patients' HIV status plays no role with respect to the MTC lineage distribution.

Mycobacterium tuberculosis Uganda II is more susceptible to rifampicin and isoniazid compared to Beijing and Delhi/CAS families

“…Although response to first-line anti-TB drugs would offer a better explanation to predominance, in this study, we have found no significant differences in the susceptibility patterns between M. tuberculosis Uganda II vs. Beijing or Delhi/CAS families, except at high drug concentrations under oxygen-depleted conditions where the Uganda II family was instead more susceptible to Rifampicin and Isoniazid than the Beijing or Delhi/CAS families. Therefore, in line with recent studies [ 4 – 6 ], the predominance of M. tuberculosis Uganda family in Uganda may not be attributed to its susceptibility behavior once exposed to Isoniazid and Rifampicin.…”

“…Chromosomal DNA used as templates in PCRs was prepared as previously described [ 17 ]. Similarly, the M. tuberculosis spoligotypes were determined by Spoligotyping as recently described [ 6 ]. The isolates were re-genotyped using MIRU-VNTR [ 17 ] and SNP-based Real-Time PCR [ 8 ] to confirm that they belong to M. tuberculosis Beijing, Uganda II and Delhi/CAS families.…”

A First Insight into the Genetic Diversity and Drug Susceptibility Pattern of Mycobacterium tuberculosis Complex in Zhejiang, China

“…Different non-Beijing sublineages exhibit diverse correlation with drug susceptibility, even within the same sublineage. A recent study from Lukoye et al has revealed that the T2 MTB genotype is associated with anti-TB drug resistance [36], whereas T2 family strains isolated from Uganda has no statistical relation with anti-TB drug resistance [37]. We hypothesize that this difference is due to the poor discriminatory power of spoligotyping.…”

Molecular epidemiology and drug sensitivity pattern of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in and around Ambo Town, Central Ethiopia

“…Mihret et al [ 19 ] reported a high proportion of T and CAS family strains in Addis Ababa while, Derbew et al [ 20 ] reported a higher prevalence of T family strains in the southern region of Ethiopia. T1 and CAS families have similarly been reported to be frequent in other African countries such as Tanzania and Uganda [ 21 , 22 ]. This indicates a possible broad distribution of these M .…”