“…Therefore, we tested several other PKMTs to investigate if an alternative enzyme could catalyze H4K12 monomethylation. A panel of different enzymes, NSD1 (Kudithipudi et al, 2014a ), NSD2 (Khella et al, 2023 ), SMYD2 (Weirich et al, 2020 ), SET7/9 (Dhayalan et al, 2011 ), SETD6 (Kublanovsky et al, 2023 ), SET8 (Kudithipudi et al, 2012 ), SUV39H1 (Kudithipudi et al, 2017 ) and SUV39H2 (Schuhmacher et al, 2015 ) which are all well characterized in our lab, were overexpressed and purified by affinity chromatography. SDS‐PAGE analysis indicated good yield and quality for all of them (Figure S4a ).…”