The T-cell costimulatory molecules B7-1 (CD80) and B7-2 (CD86) when expressed on keratinocytes deliver different signals during contact hypersensitivity responses

Burns, Robert P.; Nasir, Adnan; Ferbel, Barbara; Ramirez, Diana; Barth, Richard K.; Gaspari, Anthony A.

doi:10.1016/s0923-1811(98)83028-4

Cited by 4 publications

(9 citation statements)

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“…These data indicate the importance of keratinocyte-derived costimulation in contact dermatitis in vivo. (Nasir et al, 1994;Burns et al, 1998;Gaspari et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo

Wakem

Ramı́rez

Zlotnick

et al. 2004

J of Applied Toxicology

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Using an in vitro assay for CD80 promoter activity, the transcriptional response of primary cultured human keratinocytes from different donors in response to allergens and irritants was studied. The CD80 promoter activity was increased reproducibly after exposure to certain chemicals. The epithelial cell lines HeLa and HaCaT also increased CD80 transcriptional activity in response to exposure to a panel of different allergens and irritants. Normal or immortalized keratinocytes responded reproducibly with a unique pattern to a panel of chemicals in which the culture increases CD80 transcription after exposure to certain chemicals and has no increase in CD80 transcription after exposure to other chemicals. Some keratinocyte cultures responded positively to certain chemicals whereas others did not respond to the same chemicals. Cultured keratinocytes from 16 of 20 adult donors (80%) responded to nitrochlorobenzene in vitro by increasing CD80 transcriptional activity, thus predicting subsequent sensitization to this same hapten (chi2= 7.2, P <0.0073). Thus, a keratinocyte CD80 gene expression assay exhibits good sensitivity in predicting allergic contact dermatitis for sensitizers such as dinitrochlorobenzene. These data suggest that there is a rationale for developing the use of gene-transcription-based predictive assays to identify individual susceptibility to cutaneous reactivity after exposure to allergens and irritants.

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“…These data indicate the importance of keratinocyte-derived costimulation in contact dermatitis in vivo. (Nasir et al, 1994;Burns et al, 1998;Gaspari et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo

Wakem

Ramı́rez

Zlotnick

et al. 2004

J of Applied Toxicology

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“…This study utilized Tg mice in which keratinocytes expressed either CD80 (B7-1) or CD86 (B7-2) and compared their immune responses to primary cutaneous C. albicans infections to those of non-Tg mice. These lines of Tg mice were selected because of their exaggerated and persistent cutaneous DTH to epicutaneous haptens (2,14,36). DTH to epicutaneous haptens is a result of hapten-specific, CD4 ϩ T-lymphocytes that usually have a Th1 lymphokine profile (9,10,47).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, epicutaneous hapten challenge in B7-1 and B7-2 Tg mice results in exaggerated and persistent cutaneous DTH. In these Tg mice, the duration of ear swelling is longer than 6 weeks after a single hapten challenge and is accompanied by persistent Th1 lymphokine transcripts (2,14,36). The kinetics of DTH in B7-1 and B7-2 Tg mice are therefore profoundly altered compared to those in normal mice, in which ear swelling and Th1 lymphokine transcripts resolve within 72 h of hapten challenge.…”

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confidence: 98%

“…When either of the costimulatory molecules B7-1 (CD80) and B7-2 (CD86) is overexpressed by keratinocytes in the skin by using a keratin promoter to target these second-signal molecules to epidermis in Tg mice, an amplification of cutaneous DTH to topical haptens is seen (2,14,36). The stable expression of B7-1 or B7-2 on the cell surfaces of epidermal keratinocytes induces it to act as a professional antigen presenting cell (APC) for CD4 ϩ Tlymphocyte activation (2,14,15,36), thus amplifying antigen presentation in the skin and resulting in exaggerated cutaneous DTH. This is in contrast to keratinocytes from non-Tg mice (16), which normally act as nonprofessional APCs for T-lymphocyte activation because they cannot deliver costimulatory signals to Th lymphocytes during antigen presentation.…”

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confidence: 99%

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CD86 (B7-2), but Not CD80 (B7-1), Expression in the Epidermis of Transgenic Mice Enhances the Immunogenicity of Primary CutaneousCandida albicans Infections

Gaspari

Burns²,

Nasir³

et al. 1998

Infect. Immun.

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Transgenic (Tg) mice whose epidermal keratinocytes constitutively overexpress either B7-1 (CD80) or B7-2 (CD86) exhibited exaggerated cutaneous delayed type hypersensitivity (DTH) to haptens compared to non-Tg mice. To determine whether enhanced DTH in these Tg mice is seen in response to cutaneous fungal infections, a primary infection with Candida albicans was established by inoculating this organism on the occluded skin of Tg and non-Tg mice. These infections resolved 7 days after removal of occlusive dressing in all three groups of mice, without evidence of exaggerated inflammation in either the Tg or non-Tg mice. Only B7-2 Tg mice developed enhanced Th1-lymphocyte-mediated immune responses to C. albicans antigens after resolving this infection: enhanced footpad swelling in response to intradermal C. albicans antigens, enhanced production of mRNA encoding Th1 lymphokines in draining lymph nodes, and increased gamma interferon secreted into culture supernatants by lymph node T lymphocytes stimulated with Candida antigens in vitro. Lastly, Western blotting of sera from mice that had resolved this fungal infection indicated that only B7-2 Tg mice recognized a wide range of Candida-associated antigens. These data suggest that these two costimulatory molecules, when expressed by keratinocytes, do not deliver identical signals to C. albicans antigen-reactive Th1 lymphocytes. The enhanced immune response in B7-2 Tg mice to a cutaneous C. albicans infection demonstrates the importance of antigen presentation and costimulation in immune reactivity to fungi. Furthermore, B7-2 Tg mice may be useful in identification of protective Candida antigens.

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“…Irritant dermatitis is often caused by soaps, detergents, bleach, acids, alkali, foods, saliva, genitourinary waste, and chemicals (1)(2)(3)(4)(222)(223)(224)(225)(226)(227)(228)(229)(230)(231)(232)269,(300)(301)(302)(303)(304)(305)(306)(307)(308)(309). The severity of the dermatitis depends on host resistant, concentration of irritant, and duration of exposure.…”

Section: Contact Dermatitidesmentioning

confidence: 99%

Toxicology of the Skin

Monteiro‐Riviere¹

2010

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The T-cell costimulatory molecules B7-1 (CD80) and B7-2 (CD86) when expressed on keratinocytes deliver different signals during contact hypersensitivity responses

Cited by 4 publications

References 0 publications

Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo

Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo

CD86 (B7-2), but Not CD80 (B7-1), Expression in the Epidermis of Transgenic Mice Enhances the Immunogenicity of Primary CutaneousCandida albicans Infections

Toxicology of the Skin

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